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[172721_x_at]

Description C09G12.8B /REP_DB=WormBase Gene ID /WP=CE16833 /GEN=rac-1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_high1_10_10 > 20      6grp_total > 35      4grp_total > 10      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 110.4
(-1.9)
A 		90.2
(-3)
P 		90.1
(-3)
A 		196.8
(3)
P 		107
B 89.1
(-3)
A 		170.7
(0.9)
P 		137.6
(-0.7)
M 		213.1
(3)
P 		124
C 133.3
(-1.7)
P 		118.6
(-2.3)
A 		99.9
(-3)
A 		250.5
(3)
P 		151
avr 110.9
(-2.9) 		126.5
(-2.1) 		109.2
(-3) 		220.1
(3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 126.6
(2.3)
P 		106.8
(0.2)
P 		133.3
(3)
P 		81.7
(-2.5)
A 		77.4
(-3)
A 		103.9
(-0.2)
A 		56
B 128.4
(3)
P 		116.2
(1.5)
P 		117.3
(1.6)
M 		79.4
(-3)
A 		83.4
(-2.5)
P 		124.2
(2.5)
P 		49
C 134.8
(2.9)
M 		135.5
(3)
P 		104.4
(0.1)
A 		132.1
(2.7)
P 		70.1
(-3)
A 		111.4
(0.8)
P 		65
avr 129.9
(3) 		119.5
(1.8) 		118.3
(1.7) 		97.7
(-0.7) 		77
(-3) 		113.2
(1.1) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 40.2
(3)
A 		19.6
(-3)
P 		23.6
(-1.8)
A 		28.8
(-0.3)
P 		21
B 28.4
(-3)
A 		42.2
(1.9)
P 		31.9
(-1.8)
M 		45.4
(3)
P 		17
C 44.1
(3)
P 		34.1
(-3)
A 		34.8
(-2.6)
A 		42.2
(1.9)
P 		10
avr 37.6
(2.2) 		32
(-1.7) 		30.1
(-3) 		38.8
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 23.3
(-2.1)
P 		38
(3)
P 		29
(-0.1)
P 		20.7
(-3)
A 		23.4
(-2.1)
A 		25.3
(-1.4)
A 		17
B 44.7
(2.6)
P 		38.7
(1.2)
P 		28.5
(-1.2)
M 		21
(-3)
A 		33.9
(0.1)
P 		46.3
(3)
P 		25
C 49.9
(3)
M 		41.3
(1.1)
P 		26.6
(-2.1)
A 		26.6
(-2.1)
P 		22.7
(-3)
A 		37.3
(0.2)
P 		27
avr 39.3
(3) 		39.3
(3) 		28
(-1.1) 		22.8
(-3) 		26.7
(-1.6) 		36.3
(1.9) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172721_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11255
Sequence Type Exemplar sequence
Representative Public ID C09G12.8B NCBI
Target Description C09G12.8B /REP_DB=WormBase Gene ID /WP=CE16833 /GEN=rac-1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067962(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067962 NCBI Caenorhabditis elegans CEll Death abnormality family member (ced-10) (ced-10) mRNA, complete cds. 11/11 None
GENEFINDER00000001989 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3507048:3509490:-1 11/11 None
C09G12.8b ENSEMBL cdna:known chromosome:CEL140:IV:3506397:3509524:-1 gene:C09G12.8 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067961 5/11 Cross Hyb Matching Probes A 
C09G12.8a 5/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177111 2 1 NM_067962 172721_x_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3507381-3509496(-) 100.0 100.0
Public Domain and Genome References
Ensembl C09G12.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25821 Cell death abnormality protein 10, isoform b [Caenorhabditis elegans] ref|NP_500363.1| CEll Death abnormality family member (ced-10) [Caenorhabditis elegans] gb|AAF33846.1| cell-corpse engulfment protein CED-10 [Caenorhabditis elegans] sp|Q03206|RAC1_CAEEL RAS-related protein rac-1 (Cell-corpse engulfment protein ced-10) (CErac1) 1.0E-108
blast CAA48506 small ras-related protein [Caenorhabditis elegans] gb|AAA28141.1| rac1 protein gb|AAA28140.1| rac1 protein 1.0E-107
blast AAC25821 Cell death abnormality protein 10, isoform b [Caenorhabditis elegans] ref|NP_500363.1| CEll Death abnormality family member (ced-10) [Caenorhabditis elegans] gb|AAF33846.1| cell-corpse engulfment protein CED-10 [Caenorhabditis elegans] sp|Q03206|RAC1_CAEEL RAS-related protein rac-1 (Cell-corpse engulfment protein ced-10) (CErac1) 1.0E-105
blast CAA48506 small ras-related protein [Caenorhabditis elegans] gb|AAA28141.1| rac1 protein gb|AAA28140.1| rac1 protein 1.0E-105
blast CAB05247 Hypothetical protein K03D3.10a [Caenorhabditis elegans] ref|NP_502936.1| RAC related family member (rac-2) [Caenorhabditis elegans] sp|Q94124|RAC2_CAEEL RAS-related protein rac-2 gb|AAB40386.1| CeRac2 9.0E-97
blast CAB05247 Hypothetical protein K03D3.10a [Caenorhabditis elegans] ref|NP_502936.1| RAC related family member (rac-2) [Caenorhabditis elegans] sp|Q94124|RAC2_CAEEL RAS-related protein rac-2 gb|AAB40386.1| CeRac2 4.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.8E-90
Sequence
Target Sequence 
>C. ELEGANS:172721_X_AT
taaaacgtgtctcctgatatcctacaccacaaacgcatttcccggagaatatattccgac
ggtattcgacaactactcagcaaatgtgatggtcgacggtcggccgataaatctcgggct
ctgggatacagctggacaggaagattacgatcgactccgaccactgtcatatccacaaac
agacgtgtttctcgtatgctttgccctgaacaatccggcgagttttgagaatgttcgtgc
gaaatggtatccagaagtgtcacatcattgcccgaatacgccgattattttggttggaac
gaaagctgatctgcgtgaggatcgagatactgttgaacggctccgcgaacgccggctcca
accagtgagccaaacccagggctacgtgatggcaaaggaaatcaaggctgtcaagtatct
ggagtgctcggcgctcacgcaacgtggtctgaaacaagttttcgatgaggcgatccgagc
cgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAACGTGTCTCCTGATATCCTAC 41 639 57 Antisense
GACGGTATTCGACAACTACTCAGCA 190 377 114 Antisense
GGTCGGCCGATAAATCTCGGGCTCT 282 501 154 Antisense
GGAAGATTACGATCGACTCCGACCA 394 525 195 Antisense
TCCGACCACTGTCATATCCACAAAC 483 593 212 Antisense
AGACGTGTTTCTCGTATGCTTTGCC 185 71 237 Antisense
GCCCTGAACAATCCGGCGAGTTTTG 545 281 259 Antisense
AGAAGTGTCACATCATTGCCCGAAT 128 75 309 Antisense
ATACTGTTGAACGGCTCCGCGAACG 484 23 383 Antisense
GGCTGTCAAGTATCTGGAGTGCTCG 246 539 462 Antisense
TTCGATGAGGCGATCCGAGCCGTGC 387 695 517 Antisense
Sequence Source Affymetrix Proprietary Database