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[172719_x_at]

Description ZK1225.6 /REP_DB=WormBase Gene ID /WP=CE19345 /GEN=ssp-31 /TR=Q9XXL3 /GB=CAA18373.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 33      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 8.2
(-3)
A 		39.3
(-0.7)
A 		34.5
(-1)
A 		87.9
(3)
A 		80
B 12.5
(-3)
A 		62
(2.8)
A 		63.7
(3)
A 		48.2
(1.2)
A 		51
C 37.1
(-3)
A 		82.4
(3)
A 		41.6
(-2.4)
A 		43.2
(-2.2)
A 		45
avr 19.3
(-3) 		61.2
(3) 		46.6
(0.9) 		59.8
(2.8) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 357
1 2 3 4 5 6 range
A 65.4
(-2.2)
A 		45.4
(-2.7)
A 		31.1
(-3)
A 		57.7
(-2.4)
A 		204.1
(1.1)
P 		286.5
(3)
P 		255
B 37.3
(-2.9)
A 		53
(-2.6)
A 		33
(-3)
A 		40.7
(-2.8)
A 		313.1
(3)
P 		280.3
(2.3)
P 		280
C 46.9
(-2.3)
A 		44.9
(-2.3)
A 		3.9
(-3)
A 		12.3
(-2.9)
A 		199.3
(0.3)
P 		361.2
(3)
P 		357
avr 49.9
(-2.4) 		47.8
(-2.5) 		22.7
(-3) 		36.9
(-2.7) 		238.8
(1.5) 		309.3
(3) 		287
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 3
(-3)
A 		8.5
(0.3)
A 		9
(0.6)
A 		12.9
(3)
A 		10
B 4
(-3)
A 		15.3
(3)
A 		14.8
(2.7)
A 		10.3
(0.3)
A 		11
C 12.3
(-1.2)
A 		23.7
(3)
A 		14.5
(-0.4)
A 		7.3
(-3)
A 		16
avr 6.4
(-3) 		15.8
(3) 		12.8
(1.1) 		10.2
(-0.6) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 12.1
(-2.5)
A 		16.1
(-2.1)
A 		6.8
(-3)
A 		14.6
(-2.3)
A 		61.6
(2.2)
P 		69.8
(3)
P 		63
B 13
(-2.7)
A 		17.6
(-2.5)
A 		8
(-3)
A 		10.8
(-2.9)
A 		127.2
(3)
P 		104.5
(1.9)
P 		119
C 17.4
(-2.2)
A 		13.7
(-2.4)
A 		1
(-3)
A 		2.5
(-2.9)
A 		64.5
(0.2)
P 		120.9
(3)
P 		120
avr 14.2
(-2.4) 		15.8
(-2.3) 		5.3
(-3) 		9.3
(-2.7) 		84.4
(2.1) 		98.4
(3) 		93
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172719_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1065
Sequence Type Exemplar sequence
Representative Public ID ZK1225.6 NCBI
Target Description ZK1225.6 /REP_DB=WormBase Gene ID /WP=CE19345 /GEN=ssp-31 /TR=Q9XXL3 /GB=CAA18373.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060917(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060917 NCBI Caenorhabditis elegans Sperm-Specific family, class P family member (ssp-31) (ssp-31) mRNA, complete cds. 11/11 None
SNAP00000029393 ENSEMBL cdna:SNAP chromosome:CEL140:I:13227260:13227583:1 11/11 None
GENEFINDER00000029397 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13227260:13227583:1 11/11 None
ZK1225.6 ENSEMBL cdna:known chromosome:CEL140:I:13227260:13227583:1 gene:ZK1225.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_074229 1/11 Cross Hyb Matching Probes A 
SNAP00000001284 1/11 Cross Hyb Matching Probes None
GENEFINDER00000001293 1/11 Cross Hyb Matching Probes None
R08A2.3 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14713965-14714284(+) 92.28 92.28
chrI:13227190-13227514(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ssp-31
Ensembl ZK1225.6
Entrez Gene 173187 Entrez gene
SwissProt Q9XXL3 EMBL-EBI
WormBase CE19345 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA18373 Hypothetical protein ZK1225.6 [Caenorhabditis elegans] ref|NP_493318.1| Sperm-Specific family, class P family member (ssp-31) [Caenorhabditis elegans] 1.0E-54
blast CAB57904 Hypothetical protein R08A2.3 [Caenorhabditis elegans] ref|NP_506630.1| R08A2.3 [Caenorhabditis elegans] 1.0E-50
blast CAE60778 Hypothetical protein CBG04467 [Caenorhabditis briggsae] 2.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 7.7E-39
Sequence
Target Sequence 
>C. ELEGANS:172719_X_AT
cgactacccagcttctggtggttcatcaactcactacattgtctccgaatcggaatctcg
tttggcattcaaagtcaagtcgtccaacaatgaatcgtatcgtgttcgcccagtctatgg
attcgttgatgcgaagggaaaggctaagctcgaagtgaatcgtttggctggaccagcgaa
ggaggacaagcttgtcattcaatacgccgaagttccagctgatgagaccgatccgaaggc
tccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACTACCCAGCTTCTGGTGGTTCA 516 247 39 Antisense
CTGGTGGTTCATCAACTCACTACAT 181 241 53 Antisense
CTCACTACATTGTCTCCGAATCGGA 380 227 68 Antisense
CCGAATCGGAATCTCGTTTGGCATT 203 261 83 Antisense
CAATGAATCGTATCGTGTTCGCCCA 335 183 126 Antisense
TGTTCGCCCAGTCTATGGATTCGTT 595 561 141 Antisense
GAAAGGCTAAGCTCGAAGTGAATCG 254 359 176 Antisense
GTGAATCGTTTGGCTGGACCAGCGA 496 481 193 Antisense
GCTTGTCATTCAATACGCCGAAGTT 195 307 228 Antisense
TCAATACGCCGAAGTTCCAGCTGAT 333 633 237 Antisense
TGATGAGACCGATCCGAAGGCTCCG 541 565 258 Antisense
Sequence Source Affymetrix Proprietary Database