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[172692_x_at] histone H2B /// ---

Description C50F4.6 /REP_DB=WormBase Gene ID /WP=CE05471 /GEN=his-36 /TR=Q18742 /GB=CAA94741.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 47      4grp_highest_8_8 > 55      4grp_high_8_8 > 10      6grp_highest_nonorm > 92      6grp_total > 47      4grp_total > 21      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 221
1 2 3 4 range
A 380.8
(3)
P 		310.9
(-0)
P 		243.1
(-3)
P 		376.5
(2.8)
P 		138
B 258.7
(0.7)
P 		202.8
(-2)
P 		180.9
(-3)
P 		307.3
(3)
P 		126
C 310.3
(2.5)
P 		206.9
(-1.3)
P 		160.2
(-3)
P 		324.5
(3)
P 		164
avr 316.6
(2.2) 		240.2
(-1.1) 		194.7
(-3) 		336.1
(3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 427
1 2 3 4 5 6 range
A 364.9
(2.1)
P 		320.5
(1.2)
P 		244
(-0.3)
P 		109.1
(-3)
P 		157
(-2)
P 		408.4
(3)
P 		299
B 463.1
(2.2)
P 		358.6
(0.4)
P 		291.1
(-0.8)
P 		167.2
(-3)
P 		213.2
(-2.2)
P 		508
(3)
P 		341
C 398.5
(0.9)
P 		424.4
(1.3)
P 		158.5
(-2.6)
P 		132.8
(-3)
P 		173.4
(-2.4)
P 		536.4
(3)
P 		404
avr 408.8
(1.7) 		367.8
(1) 		231.2
(-1.4) 		136.4
(-3) 		181.2
(-2.2) 		484.3
(3) 		348
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 138.6
(3)
P 		67.5
(-2.1)
P 		63.6
(-2.4)
P 		55.1
(-3)
P 		84
B 82.5
(3)
P 		50.1
(-1.8)
P 		41.9
(-3)
P 		65.5
(0.5)
P 		41
C 102.7
(3)
P 		59.5
(-2.4)
P 		55.8
(-2.9)
P 		54.7
(-3)
P 		48
avr 107.9
(3) 		59
(-2.4) 		53.8
(-3) 		58.4
(-2.5) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 67.3
(-0.2)
P 		114
(3)
P 		53.1
(-1.2)
P 		27.7
(-3)
P 		47.4
(-1.6)
P 		99.4
(2)
P 		86
B 161.1
(1.8)
P 		119.4
(0.1)
P 		70.7
(-1.9)
P 		44.3
(-3)
P 		86.6
(-1.3)
P 		189.4
(3)
P 		145
C 147.7
(1.7)
P 		129.5
(1)
P 		40.3
(-2.5)
P 		26.7
(-3)
P 		56.1
(-1.8)
P 		179.6
(3)
P 		153
avr 125.4
(1.5) 		121
(1.3) 		54.7
(-1.9) 		32.9
(-3) 		63.4
(-1.5) 		156.1
(3) 		123
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172692_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11354
Sequence Type Exemplar sequence
Representative Public ID C50F4.6 NCBI
Target Description C50F4.6 /REP_DB=WormBase Gene ID /WP=CE05471 /GEN=his-36 /TR=Q18742 /GB=CAA94741.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073064(11), NM_073063(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073064 NCBI Caenorhabditis elegans HIStone family member (his-36) (his-36) mRNA, complete cds. 11/11 None
NM_073063 NCBI Caenorhabditis elegans HIStone family member (his-41) (his-41) mRNA, complete cds. 11/11 None
SNAP00000009318 ENSEMBL cdna:SNAP chromosome:CEL140:V:9549000:9549330:1 11/11 None
GENEFINDER00000009328 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9549000:9549330:1 11/11 None
C50F4.5 ENSEMBL cdna:known chromosome:CEL140:V:9548106:9549413:1 gene:C50F4.5 11/11 A 
C50F4.6 ENSEMBL cdna:known chromosome:CEL140:V:9548981:9549412:1 gene:C50F4.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9548999-9549330(+) 100.0 100.0
Public Domain and Genome References
Gene Title histone H2B

Gene Symbol his-41
his-36
Ensembl C50F4.5
C50F4.6
Entrez Gene 179340 Entrez gene
3565041 Entrez gene
SwissProt Q27484 EMBL-EBI
Q18742 EMBL-EBI
WormBase CE05470 Wormbase
CE05471 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261411_AT histone H2B, putative at
HG-U133_PLUS_2:207611_AT histone 1, H2bl hs
HG-U133A:207611_AT histone 1, H2bl hs
HG-FOCUS:207611_AT histone 1, H2bl hs
HG-U133A_2:207611_AT histone 1, H2bl hs
U133_X3P:G5579460_3P_AT histone 1, H2bl hs
HG-U95AV2:35576_F_AT histone 1, H2bl hs
MU11KSUBB:MSA.2872.0_AT histone 2, H2bb mm
MG-U74AV2:94638_AT histone 2, H2bb mm
MU11KSUBA:T25626_RC_AT Histone 2, H2be (Hist2h2be), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
7001 chromosome organization and biogenesis (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94740 Hypothetical protein C50F4.5 [Caenorhabditis elegans] ref|NP_505464.1| HIStone family member (his-41) [Caenorhabditis elegans] sp|Q27484|H2B3_CAEEL Probable histone H2B 2.0E-53
blast CAB05832 Hypothetical protein ZK131.9 [Caenorhabditis elegans] emb|CAB05830.1| Hypothetical protein ZK131.5 [Caenorhabditis elegans] emb|CAB04061.1| Hypothetical protein F08G2.1 [Caenorhabditis elegans] gb|AAC05103.1| Histone protein 34 [Caenorhabditis elegans] emb|CAA33642.1| histone protein [Caenorhabditis elegans] gb|AAK84525.1| Histone protein 29 [Caenorhabditis elegans] ref|NP_501409.1| HIStone family member (his-34) [Caenorhabditis elegans] ref|NP_501403.1| HIStone family member (his-29) [Caenorhabditis elegans] ref|NP_496897.1| HIStone family member (his-44) [Caenorhabditis elegans] ref|NP_496892.1| HIStone family member (his-11) [Caenorhabditis elegans] ref|NP_496888.1| HIStone family member (his-15) [Caenorhabditis elegans] sp|P04255|H2B1_CAEEL Histone H2B 3.0E-52
blast AAF98225 Histone protein 20 [Caenorhabditis elegans] gb|AAF98230.1| Histone protein 22 [Caenorhabditis elegans] gb|AAC48023.1| Histone protein 8 [Caenorhabditis elegans] ref|NP_505295.1| HIStone family member (his-20) [Caenorhabditis elegans] ref|NP_505197.1| HIStone family member (his-8) [Caenorhabditis elegans] ref|NP_505294.1| HIStone family member (his-22) [Caenorhabditis elegans] sp|Q27894|H2B2_CAEEL Histone H2B 3.0E-52
blast CAA94741 Hypothetical protein C50F4.6 [Caenorhabditis elegans] ref|NP_505465.1| HIStone family member (his-36) [Caenorhabditis elegans] 1.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR007125 EMBL-EBI Histone core 1.3E-54
Sequence
Target Sequence 
>C. ELEGANS:172692_X_AT
ggagatgaccaataagcaattcgatgatttgctgaaggagatgggaggtctcccagaggg
aatgaatctcgatccagagcttctcgcagttcttcgtcaagcaacaaatgccgagttcaa
caaggtggccaaggagaagtatggagatgttccagaaggttcagatgatgacgaagagca
gcaaacttcggagcagaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGATGACCAATAAGCAATTCGAT 67 519 54 Antisense
GCAATTCGATGATTTGCTGAAGGAG 204 323 69 Antisense
GCTGAAGGAGATGGGAGGTCTCCCA 703 293 84 Antisense
GGAGGTCTCCCAGAGGGAATGAATC 49 517 97 Antisense
ATGAATCTCGATCCAGAGCTTCTCG 20 45 115 Antisense
TCTCGCAGTTCTTCGTCAAGCAACA 510 611 135 Antisense
CTTCGTCAAGCAACAAATGCCGAGT 406 217 145 Antisense
AATGCCGAGTTCAACAAGGTGGCCA 400 167 160 Antisense
GAGTTCAACAAGGTGGCCAAGGAGA 700 395 166 Antisense
GTTCCAGAAGGTTCAGATGATGACG 108 441 202 Antisense
AGAGCAGCAAACTTCGGAGCAGAAT 210 69 228 Antisense
Sequence Source Affymetrix Proprietary Database