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[172683_x_at]

Description F22D6.13 /REP_DB=WormBase Gene ID /WP=CE05694 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) TR:Q19732 protein_id:CAA95819.1
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 90      6grp_total > 55      4grp_total > 07      6grp_cgc > 45      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 171.3
(3)
P 		99.9
(-3)
P 		133
(-0.2)
P 		159.8
(2)
P 		71
B 135.9
(2.8)
P 		116.8
(-3)
P 		136.5
(3)
P 		121
(-1.7)
P 		20
C 147.6
(0.7)
P 		98.4
(-3)
P 		156.1
(1.3)
P 		178.2
(3)
P 		80
avr 151.6
(2.8) 		105
(-3) 		141.9
(1.6) 		153
(3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 129.4
(-1.2)
P 		125.1
(-1.7)
P 		169.8
(3)
P 		119.3
(-2.3)
P 		150.5
(1)
P 		112.7
(-3)
P 		57
B 158.6
(1.3)
P 		128.6
(-0.8)
P 		182.6
(3)
P 		97.1
(-3)
P 		124.5
(-1.1)
P 		160.2
(1.4)
P 		86
C 111.2
(-2)
P 		188.6
(3)
P 		96.2
(-3)
P 		127.8
(-0.9)
P 		98.5
(-2.9)
P 		170.5
(1.8)
P 		92
avr 133.1
(0.2) 		147.4
(2.6) 		149.5
(3) 		114.7
(-3) 		124.5
(-1.3) 		147.8
(2.7) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 62.4
(3)
P 		21.7
(-3)
P 		34.8
(-1.1)
P 		23.4
(-2.7)
P 		41
B 43.3
(3)
P 		28.8
(-2)
P 		31.6
(-1)
P 		25.8
(-3)
P 		18
C 48.9
(1.7)
P 		28.3
(-3)
P 		54.4
(3)
P 		30
(-2.6)
P 		26
avr 51.5
(3) 		26.3
(-3) 		40.3
(0.3) 		26.4
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 23.9
(-3)
P 		44.5
(2.7)
P 		37
(0.7)
P 		30.3
(-1.2)
P 		45.4
(3)
P 		27.5
(-2)
P 		22
B 55.2
(2.2)
P 		42.8
(0)
P 		44.3
(0.3)
P 		25.7
(-3)
P 		50.6
(1.4)
P 		59.7
(3)
P 		34
C 41.2
(0)
P 		57.6
(3)
P 		24.5
(-3)
P 		25.7
(-2.8)
P 		31.9
(-1.7)
P 		57.1
(2.9)
P 		33
avr 40.1
(0.7) 		48.3
(3) 		35.3
(-0.7) 		27.2
(-3) 		42.6
(1.4) 		48.1
(2.9) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172683_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1621
Sequence Type Exemplar sequence
Representative Public ID F22D6.13 NCBI
Target Description F22D6.13 /REP_DB=WormBase Gene ID /WP=CE05694 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) TR:Q19732 protein_id:CAA95819.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059608(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059608 NCBI Caenorhabditis elegans F22D6.6 (F22D6.6) mRNA, complete cds. 11/11 None
F22D6.6 ENSEMBL cdna:known chromosome:CEL140:I:7103620:7106474:1 gene:F22D6.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_059610 2/11 Cross Hyb Matching Probes A B 
GENEFINDER00000013229 1/11 Cross Hyb Matching Probes None
F22D6.7 2/11 Cross Hyb Matching Probes A B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7106244-7106430(+) 100.0 100.0
chrIII:6982035-6982221(+) 98.39 98.39
chrIII:4749292-4749477(+) 97.85 97.85
chrIII:11939224-11939410(+) 97.85 97.85
chrI:7107767-7107964(-) 85.48 96.77
chrIII:11940041-11940227(-) 97.85 97.85
chrIII:6985164-6985361(-) 87.1 98.39
chrIII:4752610-4752785(-) 93.01 93.01
Public Domain and Genome References
Gene Title
Gene Symbol ekl-1
Ensembl F22D6.6
Entrez Gene 172444 Entrez gene
SwissProt Q19731 EMBL-EBI
WormBase CE05687 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA95818 Hypothetical protein F22D6.6 [Caenorhabditis elegans] ref|NP_492009.1| F22D6.6 [Caenorhabditis elegans] 0.0
blast CAE73096 Hypothetical protein CBG20475 [Caenorhabditis briggsae] 0.0
blast CAE70293 Hypothetical protein CBG16814 [Caenorhabditis briggsae] 5.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR008191 EMBL-EBI Maternal tudor protein 4.3E-21
Pfam IPR008191 EMBL-EBI Maternal tudor protein 2.9E-16
Sequence
Target Sequence 
>C. ELEGANS:172683_X_AT
gcaagtgaatttctcgacatttctcgacaatttttttgcaatgcaagtgtgcggagaaat
gacgagaattgacgtgaaattcgcaatttctccgcatttctcgacatccacacataccgt
agttggggacgttgttggcggcaaatcgcggcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAGTGAATTTCTCGACATTTCTC 334 321 15 Antisense
GAATTTCTCGACATTTCTCGACAAT 637 325 21 Antisense
CGACATTTCTCGACAATTTTTTTGC 188 251 29 Antisense
TTCTCGACAATTTTTTTGCAATGCA 670 691 35 Antisense
TTTTGCAATGCAAGTGTGCGGAGAA 576 677 48 Antisense
GCAAGTGTGCGGAGAAATGACGAGA 419 321 57 Antisense
GACGAGAATTGACGTGAAATTCGCA 568 377 75 Antisense
GAAATTCGCAATTTCTCCGCATTTC 542 363 90 Antisense
GACATCCACACATACCGTAGTTGGG 78 371 117 Antisense
GTAGTTGGGGACGTTGTTGGCGGCA 520 257 133 Antisense
CGTTGTTGGCGGCAAATCGCGGCGA 267 247 144 Antisense
Sequence Source Affymetrix Proprietary Database