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[172653_at]

Description C28H8.2 /REP_DB=WormBase Gene ID /WP=CE01824 /TR=SW:Q09243 /GB=AAA62290.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 1.6
(-3)
A 		30.2
(-0.7)
A 		17.6
(-1.7)
A 		76.6
(3)
P 		75
B 1.9
(-3)
A 		16.2
(-1.6)
A 		3.2
(-2.9)
A 		63.4
(3)
P 		62
C 21.6
(-1.4)
A 		28
(-0.6)
A 		6.9
(-3)
A 		60.4
(3)
P 		54
avr 8.4
(-3) 		24.8
(-1.3) 		9.2
(-2.9) 		66.8
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 5.3
(-3)
A 		19.8
(-1.4)
A 		54.7
(2.6)
A 		58.1
(3)
P 		26.8
(-0.6)
A 		37.4
(0.6)
P 		53
B 2.6
(-3)
A 		20.2
(-0.8)
A 		44.1
(2.2)
A 		50.2
(3)
P 		38.3
(1.5)
P 		46.1
(2.5)
P 		48
C 8
(-3)
A 		33.6
(1.3)
P 		22.7
(-0.5)
A 		43
(2.8)
P 		37.4
(1.9)
P 		43.9
(3)
P 		36
avr 5.3
(-3) 		24.5
(-0.4) 		40.5
(1.7) 		50.4
(3) 		34.2
(0.8) 		42.5
(1.9) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 0.6
(-3)
A 		6.6
(0.4)
A 		4.6
(-0.7)
A 		11.2
(3)
P 		11
B 0.6
(-3)
A 		4
(-1.4)
A 		0.7
(-3)
A 		13.5
(3)
P 		13
C 7.1
(0.6)
A 		8
(1.3)
A 		2.4
(-3)
A 		10.2
(3)
P 		8
avr 2.8
(-2.9) 		6.2
(-0.6) 		2.6
(-3) 		11.6
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 1
(-3)
A 		7
(-0.4)
A 		11.9
(1.8)
A 		14.7
(3)
P 		8.1
(0.1)
A 		9.1
(0.5)
P 		14
B 0.9
(-3)
A 		6.7
(-0.9)
A 		10.7
(0.6)
A 		13.3
(1.6)
P 		15.6
(2.4)
P 		17.2
(3)
P 		16
C 3
(-3)
A 		10.2
(0.7)
P 		5.8
(-1.6)
A 		8.7
(-0.1)
P 		12.1
(1.7)
P 		14.7
(3)
P 		12
avr 1.6
(-3) 		8
(0.2) 		9.5
(0.9) 		12.2
(2.3) 		11.9
(2.1) 		13.7
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172653_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7928
Sequence Type Exemplar sequence
Representative Public ID C28H8.2 NCBI
Target Description C28H8.2 /REP_DB=WormBase Gene ID /WP=CE01824 /TR=SW:Q09243 /GB=AAA62290.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065884(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065884 NCBI Caenorhabditis elegans C28H8.2 (C28H8.2) mRNA, complete cds. 11/11 None
SNAP00000005437 ENSEMBL cdna:SNAP chromosome:CEL140:III:5923299:5924346:1 11/11 None
C28H8.2 ENSEMBL cdna:known chromosome:CEL140:III:5923136:5924380:1 gene:C28H8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5924144-5924345(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C28H8.2
Entrez Gene 182995 Entrez gene
SwissProt Q09243 EMBL-EBI
WormBase CE32812 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498285 C28H8.2 [Caenorhabditis elegans] gb|AAA62290.2| Hypothetical protein C28H8.2 [Caenorhabditis elegans] sp|Q09243|YP92_CAEEL Hypothetical protein C28H8.2 in chromosome III precursor 8.0E-94
blast CAE64360 Hypothetical protein CBG09047 [Caenorhabditis briggsae] 1.0E-52
Sequence
Target Sequence 
>C. ELEGANS:172653_AT
atgactaaagattcttctcatatgacggttgaattccatgttacatcacaatcttggttt
ggattcgaagatcattttcatatgattatcagagcggttaatgacgagaatgtcggatgg
ggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACTAAAGATTCTTCTCATATGA 449 45 13 Antisense
AAGATTCTTCTCATATGACGGTTGA 31 157 20 Antisense
TGACGGTTGAATTCCATGTTACATC 8 571 35 Antisense
CGGTTGAATTCCATGTTACATCACA 516 243 38 Antisense
GAATTCCATGTTACATCACAATCTT 665 323 43 Antisense
GTTACATCACAATCTTGGTTTGGAT 168 447 52 Antisense
ATCACAATCTTGGTTTGGATTCGAA 595 27 57 Antisense
TGGATTCGAAGATCATTTTCATATG 338 551 72 Antisense
TTTTCATATGATTATCAGAGCGGTT 255 675 87 Antisense
CAGAGCGGTTAATGACGAGAATGTC 634 203 102 Antisense
ATGACGAGAATGTCGGATGGGGAGT 142 43 113 Antisense
Sequence Source Affymetrix Proprietary Database