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[172631_x_at]

Description Y25C1A.7A /REP_DB=WormBase Gene ID /WP=CE21474 /TR=Q9TYM2 /GB=AAD12838.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 97      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 82      6grp_total > 86      4grp_total > 60      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 450
1 2 3 4 range
A 567.5
(3)
P 		487.8
(1)
P 		466.1
(0.4)
P 		333.1
(-3)
P 		234
B 487
(2.7)
P 		495.1
(3)
P 		444.7
(1)
P 		342.6
(-3)
P 		153
C 547.9
(-1.2)
P 		564.9
(-0.9)
P 		782.6
(3)
P 		448.3
(-3)
P 		334
avr 534.1
(2) 		515.9
(1.5) 		564.5
(3) 		374.7
(-3) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 205.9
(-3)
P 		405.5
(0.7)
P 		250.4
(-2.2)
P 		425.2
(1.1)
P 		297.7
(-1.3)
P 		525.6
(3)
P 		320
B 180.8
(-3)
P 		279.6
(0.3)
P 		197.9
(-2.4)
P 		292.5
(0.7)
P 		360.4
(3)
P 		332.1
(2.1)
P 		180
C 459.4
(2.5)
P 		189.2
(-3)
P 		425
(1.8)
P 		327.6
(-0.2)
P 		438.3
(2.1)
P 		483.3
(3)
P 		294
avr 282
(-3) 		291.4
(-2.7) 		291.1
(-2.7) 		348.4
(-0.6) 		365.5
(0) 		447
(3) 		165
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 224
1 2 3 4 range
A 206.6
(3)
P 		105.9
(-0.8)
P 		121.8
(-0.2)
P 		48.8
(-3)
P 		158
B 155.3
(3)
P 		122.3
(0.6)
P 		103
(-0.8)
P 		73
(-3)
P 		82
C 181.4
(0.2)
P 		162.4
(-0.4)
P 		272.8
(3)
P 		75.6
(-3)
P 		197
avr 181.1
(3) 		130.2
(0.4) 		165.9
(2.2) 		65.8
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 38
(-3)
P 		144.2
(3)
P 		54.5
(-2.1)
P 		107.8
(0.9)
P 		89.8
(-0.1)
P 		128
(2.1)
P 		106
B 62.9
(-2.1)
P 		93.1
(-0.3)
P 		48.1
(-3)
P 		77.5
(-1.2)
P 		146.4
(3)
P 		123.9
(1.6)
P 		98
C 170.3
(3)
P 		57.7
(-3)
P 		108.2
(-0.3)
P 		65.9
(-2.6)
P 		141.8
(1.5)
P 		161.8
(2.5)
P 		113
avr 90.4
(-1.2) 		98.3
(-0.5) 		70.3
(-3) 		83.7
(-1.8) 		126
(1.9) 		137.9
(3) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172631_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5046
Sequence Type Exemplar sequence
Representative Public ID Y25C1A.7A NCBI
Target Description Y25C1A.7A /REP_DB=WormBase Gene ID /WP=CE21474 /TR=Q9TYM2 /GB=AAD12838.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027249(10), NM_062037(10), NM_062038(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027249 NCBI Caenorhabditis elegans Y25C1A.7c (Y25C1A.7) mRNA, complete cds. 10/11 None
NM_062037 NCBI Caenorhabditis elegans Y25C1A.7a (Y25C1A.7) mRNA, complete cds. 10/11 A 
NM_062038 NCBI Caenorhabditis elegans Y25C1A.7b (Y25C1A.7) mRNA, complete cds. 10/11 A 
GENEFINDER00000041892 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3065229:3070937:-1 10/11 None
Y25C1A.7a.1 ENSEMBL cdna:known chromosome:CEL140:II:3062544:3071057:-1 gene:Y25C1A.7 11/11 None
Y25C1A.7a.2 ENSEMBL cdna:known chromosome:CEL140:II:3062544:3071059:-1 gene:Y25C1A.7 11/11 None
Y25C1A.7b ENSEMBL cdna:known chromosome:CEL140:II:3065229:3070937:-1 gene:Y25C1A.7 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041877 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3065227-3071031(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y25C1A.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022420 Y25C1A.7c [Caenorhabditis elegans] gb|AAX88818.1| Hypothetical protein Y25C1A.7c [Caenorhabditis elegans] 0.0
blast T33904 hypothetical protein Y25C1A.7a - Caenorhabditis elegans 0.0
blast NP_494439 Y25C1A.7b [Caenorhabditis elegans] gb|AAD12839.1| Hypothetical protein Y25C1A.7b [Caenorhabditis elegans] 1.0E-165
blast NP_494439 Y25C1A.7b [Caenorhabditis elegans] gb|AAD12839.1| Hypothetical protein Y25C1A.7b [Caenorhabditis elegans] 1.0E-160
blast NP_494438 Y25C1A.7a [Caenorhabditis elegans] gb|AAX88817.1| Hypothetical protein Y25C1A.7a [Caenorhabditis elegans] 1.0E-158
blast NP_494438 Y25C1A.7a [Caenorhabditis elegans] gb|AAX88817.1| Hypothetical protein Y25C1A.7a [Caenorhabditis elegans] 1.0E-154
blast NP_001022420 Y25C1A.7c [Caenorhabditis elegans] gb|AAX88818.1| Hypothetical protein Y25C1A.7c [Caenorhabditis elegans] 1.0E-154
blast NP_494439 Y25C1A.7b [Caenorhabditis elegans] gb|AAD12839.1| Hypothetical protein Y25C1A.7b [Caenorhabditis elegans] 1.0E-154
Protein Domains
Database ID Description E-Value
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 6.0E-65
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 1.7E-72
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 1.7E-72
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 6.2E-31
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 2.1E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022420.1 5 108-130,145-167,180-202,206-228,241-263
NP_494438.2 5 108-130,145-167,180-202,206-228,241-263
NP_494439.1 5 118-140,155-177,190-212,216-238,251-273
Y25C1A.7a.1 5 107-129,232-254,264-286,291-313,323-345
Y25C1A.7a.2 5 107-129,232-254,264-286,291-313,323-345
Y25C1A.7b 5 118-140,155-177,190-212,216-238,251-273
GENEFINDER00000041892 5 150-172,187-209,222-244,248-270,283-305
Sequence
Target Sequence 
>C. ELEGANS:172631_X_AT
atagccccagtttttgcaatttgtcacacggttttttgaccaacggaaacttgaaaactg
gcagtttttcagagcaaaaaatgaatttttatacaagtttcataacctctgcatctacat
taatcttcctgtacgtcgtcattgttccacttcttctctacggacttttatggaatcgcc
gtagtgagcttcttcatccatacgttgatcttgtctgtctctatggatactcgttggcta
tttttattcctgtttcagttctctggattgttgacattggatggttccgttgggctctga
tcttcgcgtcggttggactttcgggaactgtgctcgccagagcaatttggccagccgtac
agaatgataacaacaaaatgatcgcattcggtaccgtaatcagtgtcgtcgtgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAGCCCCAGTTTTTGCAATTTGTC 265 21 602 Antisense
ACCTCTGCATCTACATTAATCTTCC 60 89 706 Antisense
TTCCTGTACGTCGTCATTGTTCCAC 48 699 727 Antisense
CACTTCTTCTCTACGGACTTTTATG 132 197 749 Antisense
TGGAATCGCCGTAGTGAGCTTCTTC 170 547 772 Antisense
CATACGTTGATCTTGTCTGTCTCTA 650 213 800 Antisense
CTGTCTCTATGGATACTCGTTGGCT 51 239 816 Antisense
CATTGGATGGTTCCGTTGGGCTCTG 62 215 876 Antisense
GGTTGGACTTTCGGGAACTGTGCTC 194 507 912 Antisense
AAATGATCGCATTCGGTACCGTAAT 131 117 977 Antisense
TACCGTAATCAGTGTCGTCGTGCTC 414 649 993 Antisense
Sequence Source Affymetrix Proprietary Database