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[172594_x_at]

Description T12B5.10 /REP_DB=WormBase Gene ID /WP=CE20067 /TR=Q9TZ26 /GB=AAC68934.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 72      4grp_high_8_8 > 05      6grp_highest_nonorm > 72      6grp_total > 72      4grp_total > 04      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 210
1 2 3 4 range
A 178.6
(-2.3)
P 		175.3
(-2.4)
A 		160.1
(-3)
P 		308.8
(3)
P 		149
B 171.3
(-0.8)
P 		99.3
(-3)
A 		115.3
(-2.5)
P 		292.1
(3)
P 		193
C 193.1
(1.3)
P 		157
(-3)
P 		207.5
(3)
P 		198.1
(1.9)
P 		51
avr 181
(-1.2) 		143.9
(-3) 		161
(-2.2) 		266.3
(3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 609
1 2 3 4 5 6 range
A 272.2
(-1.8)
P 		173.5
(-3)
P 		679.6
(3)
P 		546.4
(1.4)
P 		454
(0.3)
P 		197.5
(-2.7)
P 		506
B 222.7
(-2.6)
P 		250.8
(-2.3)
P 		732
(3)
P 		584.8
(1.4)
P 		564
(1.1)
P 		189
(-3)
P 		543
C 123.4
(-3)
P 		266.2
(-1.5)
P 		307
(-1.1)
P 		710.2
(3)
P 		299.8
(-1.2)
P 		223.6
(-2)
P 		587
avr 206.1
(-3) 		230.2
(-2.6) 		572.9
(2.4) 		613.8
(3) 		439.3
(0.4) 		203.4
(-3) 		410
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 65
(3)
P 		38.1
(-3)
A 		41.8
(-2.2)
P 		45.2
(-1.4)
P 		27
B 54.6
(1.8)
P 		24.5
(-3)
A 		26.7
(-2.6)
P 		62.2
(3)
P 		38
C 63.9
(1.7)
P 		45.1
(-1.2)
P 		72.3
(3)
P 		33.4
(-3)
P 		39
avr 61.2
(3) 		35.9
(-3) 		46.9
(-0.4) 		46.9
(-0.4) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 50.2
(-2.9)
P 		61.7
(-2.2)
P 		148
(3)
P 		138.6
(2.4)
P 		137
(2.3)
P 		48.1
(-3)
P 		100
B 77.5
(-2.7)
P 		83.5
(-2.5)
P 		177.7
(1.1)
P 		154.9
(0.2)
P 		229.2
(3)
P 		70.5
(-3)
P 		159
C 45.7
(-3)
P 		81.2
(-0.8)
P 		78.1
(-1)
P 		142.8
(3)
P 		97
(0.2)
P 		74.9
(-1.2)
P 		97
avr 57.8
(-3) 		75.5
(-1.9) 		134.6
(1.8) 		145.4
(2.4) 		154.4
(3) 		64.5
(-2.6) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172594_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8329
Sequence Type Exemplar sequence
Representative Public ID T12B5.10 NCBI
Target Description T12B5.10 /REP_DB=WormBase Gene ID /WP=CE20067 /TR=Q9TZ26 /GB=AAC68934.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064899(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064899 NCBI Caenorhabditis elegans T12B5.10 (T12B5.10) mRNA, complete cds. 11/11 None
SNAP00000007700 ENSEMBL cdna:SNAP chromosome:CEL140:III:944860:946091:-1 11/11 None
GENEFINDER00000007711 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:944860:946265:-1 11/11 None
T12B5.10 ENSEMBL cdna:known chromosome:CEL140:III:944860:946265:-1 gene:T12B5.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064895 1/11 Cross Hyb Matching Probes A 
NM_064898 2/11 Cross Hyb Matching Probes B 
NM_064742 1/11 Cross Hyb Matching Probes A 
SNAP00000007696 1/11 Cross Hyb Matching Probes None
GENEFINDER00000007715 2/11 Cross Hyb Matching Probes None
GENEFINDER00000042038 1/11 Cross Hyb Matching Probes None
C29F9.11 1/11 Cross Hyb Matching Probes A 
T12B5.6 1/11 Cross Hyb Matching Probes A 
T12B5.8 2/11 Cross Hyb Matching Probes B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:944859-946265(-) 96.05 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.10
Entrez Gene 188448 Entrez gene
SwissProt Q9TZ26 EMBL-EBI
WormBase CE20067 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68934 Hypothetical protein T12B5.10 [Caenorhabditis elegans] ref|NP_497300.1| T12B5.10 [Caenorhabditis elegans] 0.0
blast AAC68934 Hypothetical protein T12B5.10 [Caenorhabditis elegans] ref|NP_497300.1| T12B5.10 [Caenorhabditis elegans] 1.0E-169
blast AAC68933 Hypothetical protein T12B5.8 [Caenorhabditis elegans] ref|NP_497299.2| T12B5.8 [Caenorhabditis elegans] 1.0E-124
blast AAC68933 Hypothetical protein T12B5.8 [Caenorhabditis elegans] ref|NP_497299.2| T12B5.8 [Caenorhabditis elegans] 1.0E-116
blast AAC68927 Hypothetical protein T12B5.1 [Caenorhabditis elegans] ref|NP_497307.1| T12B5.1 [Caenorhabditis elegans] 2.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 8.6E-59
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 8.5E-13
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 8.6E-59
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 5.2E-10
Sequence
Target Sequence 
>C. ELEGANS:172594_X_AT
gatattatcgaagcacgcacggaaaattgacattcggaactttacggaaaatagacgcga
cattattaaaactttcaatgagatttggaatactgaaaaatgtattctcgtaaaggaaat
aagacttcggcacttctcatttgatgaggttcttacaaatcttccatggtttaatgctaa
aaagctcaaaactattgaattaaattggatgaagtcaatagaccgttttgaacgtataac
ccaattggagcaatggaaaaatgcagaagtagttaaaattggttctaagattgccagtat
aatgattgttcatttttttcatttcaaatgcttcactattaaccatattgacgaatttcc
agcacaggctgcaatacaaatgagagatgatcttttaagaagaagtacattccaaagttg
ctatataattttttacaaatcaaagacaaatccgattgaaattgcaaaagtattcaaacc
ggactacactggcgaaaacgtatttaatatcgaatattcaaatggtaacgacaaattcga
tatttcgcttcaagagttttcatactat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATTATCGAAGCACGCACGGAAA 297 427 471 Antisense
ACTTTACGGAAAATAGACGCGACAT 602 101 509 Antisense
GACGCGACATTATTAAAACTTTCAA 273 381 524 Antisense
AAATAAGACTTCGGCACTTCTCATT 274 117 587 Antisense
GAGGTTCTTACAAATCTTCCATGGT 517 403 616 Antisense
CAAATGCTTCACTATTAACCATATT 369 193 795 Antisense
TTAACCATATTGACGAATTTCCAGC 45 683 809 Antisense
GAATTTCCAGCACAGGCTGCAATAC 590 325 823 Antisense
ATTCAAACCGGACTACACTGGCGAA 159 11 942 Antisense
CGACAAATTCGATATTTCGCTTCAA 274 253 999 Antisense
TTCGCTTCAAGAGTTTTCATACTAT 671 695 1014 Antisense
Sequence Source Affymetrix Proprietary Database