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[172592_x_at]

Description T20D4.16 /REP_DB=WormBase Gene ID /WP=CE13808 /TR=P91461 /GB=AAB37583.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 73      4grp_total > 02      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 6.2
(-3)
A 		15.9
(-2.5)
A 		24
(-2.1)
A 		122.2
(3)
A 		116
B 21.8
(-2.3)
A 		7
(-3)
A 		7.5
(-3)
A 		137.2
(3)
P 		130
C 6.6
(-2.9)
A 		4.5
(-3)
A 		7.7
(-2.9)
A 		211.3
(3)
P 		207
avr 11.5
(-2.9) 		9.1
(-3) 		13.1
(-2.8) 		156.9
(3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 21.8
(-0.9)
A 		5.5
(-3)
A 		51.6
(3)
A 		8.6
(-2.6)
A 		28.5
(-0)
A 		8
(-2.7)
A 		46
B 6.6
(-3)
A 		22
(-0.4)
A 		36.2
(1.9)
A 		43.1
(3)
A 		6.3
(-3)
A 		14.6
(-1.6)
A 		37
C 4
(-2.9)
A 		37.4
(2.5)
A 		3.1
(-3)
A 		38.8
(2.7)
A 		40.6
(3)
A 		40.1
(2.9)
A 		38
avr 10.8
(-3) 		21.6
(0.3) 		30.3
(3) 		30.2
(3) 		25.1
(1.4) 		20.9
(0.1) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 2.3
(-3)
A 		3.5
(-2.5)
A 		6.3
(-1.5)
A 		17.9
(3)
A 		16
B 6.9
(-1.9)
A 		1.7
(-3)
A 		1.7
(-3)
A 		29.2
(3)
P 		28
C 2.2
(-2.8)
A 		1.3
(-3)
A 		2.7
(-2.8)
A 		35.6
(3)
P 		34
avr 3.8
(-2.6) 		2.2
(-3) 		3.6
(-2.7) 		27.6
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 4
(-1.6)
A 		2
(-2.9)
A 		11.2
(3)
A 		2.2
(-2.8)
A 		8.6
(1.3)
A 		1.9
(-3)
A 		9
B 2.3
(-3)
A 		7.3
(0.3)
A 		8.8
(1.3)
A 		11.4
(3)
A 		2.6
(-2.8)
A 		5.4
(-1)
A 		9
C 1.5
(-2.7)
A 		11.4
(2)
A 		0.8
(-3)
A 		7.8
(0.3)
A 		13.1
(2.9)
A 		13.4
(3)
A 		13
avr 2.6
(-3) 		6.9
(1.7) 		6.9
(1.7) 		7.1
(1.9) 		8.1
(3) 		6.9
(1.7) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172592_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15650
Sequence Type Exemplar sequence
Representative Public ID T20D4.16 NCBI
Target Description T20D4.16 /REP_DB=WormBase Gene ID /WP=CE13808 /TR=P91461 /GB=AAB37583.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071546(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071546 NCBI Caenorhabditis elegans T20D4.16 (T20D4.16) mRNA, complete cds. 11/11 None
GENEFINDER00000008856 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3393325:3394514:-1 9/11 None
T20D4.16 ENSEMBL cdna:known chromosome:CEL140:V:3394215:3394514:-1 gene:T20D4.16 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071518 1/11 Cross Hyb Matching Probes A 
SNAP00000011250 1/11 Cross Hyb Matching Probes None
F35F10.13 1/11 Cross Hyb Matching Probes A 
SNAP00000008841 9/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3394214-3394514(-) 88.3 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.16
Entrez Gene 188651 Entrez gene
SwissProt P91461 EMBL-EBI
WormBase CE13808 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99928 Hypothetical protein F35F10.13 [Caenorhabditis elegans] ref|NP_503919.1| F35F10.13 [Caenorhabditis elegans] 3.0E-95
blast AAB37582 Hypothetical protein T20D4.15 [Caenorhabditis elegans] ref|NP_503946.1| T20D4.15 [Caenorhabditis elegans] 3.0E-78
blast NP_503904 T28A11.3 [Caenorhabditis elegans] gb|AAC48115.1| Hypothetical protein T28A11.3 [Caenorhabditis elegans] 1.0E-36
blast AAB37583 Hypothetical protein T20D4.16 [Caenorhabditis elegans] ref|NP_503947.1| T20D4.16 [Caenorhabditis elegans] 1.0E-24
blast AAF99928 Hypothetical protein F35F10.13 [Caenorhabditis elegans] ref|NP_503919.1| F35F10.13 [Caenorhabditis elegans] 4.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 3.8E-39
Sequence
Target Sequence 
>C. ELEGANS:172592_X_AT
tcctccaacttgccgtcattggcaccatgctgctcggattagtccatggatcttctgatg
ctgggaacggcactaaatgtagtgatggagtggttggcattgtgtgtggaaaggaagcca
cgttta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCCAACTTGCCGTCATTGGCAC 107 593 20 Antisense
CAACTTGCCGTCATTGGCACCATGC 389 187 25 Antisense
ACCATGCTGCTCGGATTAGTCCATG 46 87 43 Antisense
GCTGCTCGGATTAGTCCATGGATCT 338 297 48 Antisense
AGTCCATGGATCTTCTGATGCTGGG 402 61 60 Antisense
ATCTTCTGATGCTGGGAACGGCACT 241 31 69 Antisense
GATGCTGGGAACGGCACTAAATGTA 101 413 76 Antisense
GTAGTGATGGAGTGGTTGGCATTGT 479 453 98 Antisense
GGAGTGGTTGGCATTGTGTGTGGAA 307 517 106 Antisense
GTTGGCATTGTGTGTGGAAAGGAAG 175 425 112 Antisense
GTGTGTGGAAAGGAAGCCACGTTTA 612 483 121 Antisense
Sequence Source Affymetrix Proprietary Database