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[172589_x_at]

Description T12B5.11 /REP_DB=WormBase Gene ID /WP=CE20068 /TR=Q9TZ24 /GB=AAC68938.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_highest_8_8 > 44      4grp_high_8_8 > 16      6grp_highest_nonorm > 31      6grp_total > 23      4grp_total > 31      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 989
1 2 3 4 range
A 1418.7
(3)
P 		573
(-3)
P 		698
(-2.1)
P 		984.8
(-0.1)
P 		846
B 1446.3
(3)
P 		681.6
(-2.3)
P 		577.7
(-3)
P 		1179.2
(1.2)
P 		869
C 1562.4
(3)
P 		702.1
(-3)
P 		764.9
(-2.6)
P 		1095
(-0.3)
P 		860
avr 1475.8
(3) 		652.2
(-3) 		680.2
(-2.8) 		1086.3
(0.2) 		824

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 995
1 2 3 4 5 6 range
A 1120.7
(1.9)
P 		1186.7
(2.4)
P 		1277.9
(3)
P 		1141.7
(2.1)
P 		659.5
(-1.3)
P 		411.4
(-3)
P 		867
B 1405.9
(3)
P 		1149.1
(1.2)
P 		1198.6
(1.6)
P 		734.7
(-1.7)
P 		777.6
(-1.4)
P 		547.3
(-3)
P 		859
C 1389.9
(3)
P 		1236.9
(1.9)
P 		1073.4
(0.8)
P 		838
(-0.9)
P 		678.9
(-2)
P 		531.8
(-3)
P 		858
avr 1305.5
(3) 		1190.9
(2.1) 		1183.3
(2.1) 		904.8
(0) 		705.3
(-1.5) 		496.8
(-3) 		809
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 393
1 2 3 4 range
A 516.6
(3)
P 		124.4
(-3)
P 		182.5
(-2.1)
P 		144.2
(-2.7)
P 		392
B 461.2
(3)
P 		168.3
(-2.4)
P 		133.9
(-3)
P 		251.3
(-0.8)
P 		327
C 517.2
(3)
P 		201.9
(-2.7)
P 		266.6
(-1.5)
P 		184.6
(-3)
P 		333
avr 498.3
(3) 		164.9
(-3) 		194.3
(-2.5) 		193.4
(-2.5) 		333

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 415
1 2 3 4 5 6 range
A 206.6
(-1)
P 		422.1
(3)
P 		278.3
(0.3)
P 		289.6
(0.5)
P 		199
(-1.2)
P 		100.2
(-3)
P 		322
B 489.2
(3)
P 		382.5
(0.8)
P 		291.1
(-1)
P 		194.6
(-3)
P 		315.9
(-0.5)
P 		204.1
(-2.8)
P 		295
C 515.2
(3)
P 		377.4
(0.6)
P 		273.2
(-1.2)
P 		168.5
(-3)
P 		219.7
(-2.1)
P 		178
(-2.8)
P 		347
avr 403.7
(3) 		394
(2.8) 		280.9
(-0) 		217.6
(-1.6) 		244.9
(-0.9) 		160.8
(-3) 		243
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172589_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8269
Sequence Type Exemplar sequence
Representative Public ID T12B5.11 NCBI
Target Description T12B5.11 /REP_DB=WormBase Gene ID /WP=CE20068 /TR=Q9TZ24 /GB=AAC68938.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064901(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064901 NCBI Caenorhabditis elegans T12B5.11 (T12B5.11) mRNA, complete cds. 11/11 None
SNAP00000007701 ENSEMBL cdna:SNAP chromosome:CEL140:III:949350:954608:-1 11/11 None
GENEFINDER00000007716 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:949350:950485:-1 11/11 None
T12B5.11 ENSEMBL cdna:known chromosome:CEL140:III:949314:950485:-1 gene:T12B5.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062259 3/11 Cross Hyb Matching Probes A 
F54D10.2 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:949349-950485(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.11
Entrez Gene 175258 Entrez gene
SwissProt Q9TZ24 EMBL-EBI
WormBase CE20068 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 0.0
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 1.0E-126
blast AAC68783 Hypothetical protein F54D10.2 [Caenorhabditis elegans] ref|NP_494660.2| F54D10.2 [Caenorhabditis elegans] 3.0E-89
blast AAC68783 Hypothetical protein F54D10.2 [Caenorhabditis elegans] ref|NP_494660.2| F54D10.2 [Caenorhabditis elegans] 8.0E-84
blast CAB03494 Hypothetical protein ZC47.6 [Caenorhabditis elegans] ref|NP_497363.1| ZC47.6 [Caenorhabditis elegans] 7.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.8E-66
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.8E-66
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 7.1E-13
Sequence
Target Sequence 
>C. ELEGANS:172589_X_AT
aaaacttgtcaatttctcattcaatgatgtcatcactattctccagtattgtaacaccaa
agttctggaaagcattgcattgtgggatactcacataaacaggcagtttgaaaatattgc
tcatctggatcaatggaaatgtgcgaaaaaatgcagtctgtttggtgataagcttgaaaa
taaactaattgaccatctgttccactttcaatggttccagatccaactggacgactttcc
aacatatattgcaatcaaaattagagatgatctaatgagaagaagtacatttcaaggttg
ccagatatattgtgacacatcaaagtcaaatccaattgaaattgcaaaagtgttcaaacc
ggactatgatggcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAACTTGTCAATTTCTCATTCAAT 212 131 501 Antisense
AATGATGTCATCACTATTCTCCAGT 441 165 523 Antisense
CTATTCTCCAGTATTGTAACACCAA 564 223 536 Antisense
GAAAGCATTGCATTGTGGGATACTC 463 359 568 Antisense
GCAGTCTGTTTGGTGATAAGCTTGA 95 313 653 Antisense
ACTAATTGACCATCTGTTCCACTTT 351 101 684 Antisense
GTTCCACTTTCAATGGTTCCAGATC 50 441 699 Antisense
GATCCAACTGGACGACTTTCCAACA 576 415 720 Antisense
GAAGTACATTTCAAGGTTGCCAGAT 272 335 782 Antisense
GGTTGCCAGATATATTGTGACACAT 438 507 796 Antisense
GTGTTCAAACCGGACTATGATGGCG 332 483 850 Antisense
Sequence Source Affymetrix Proprietary Database