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[172579_x_at]

Description F59E12.6 /REP_DB=WormBase Gene ID /WP=CE11528 /TR=O01895 /GB=AAB54256.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 35      4grp_highest_8_8 > 53      4grp_high_8_8 > 08      6grp_highest_nonorm > 61      6grp_total > 35      4grp_total > 31      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 418
1 2 3 4 range
A 501.5
(3)
P 		282
(-2.3)
P 		251.5
(-3)
P 		396
(0.5)
P 		250
B 607.7
(3)
P 		189.3
(-3)
P 		232.7
(-2.4)
P 		409.4
(0.2)
P 		418
C 485.6
(3)
P 		192.2
(-3)
P 		239.3
(-2)
P 		399.9
(1.2)
P 		293
avr 531.6
(3) 		221.2
(-3) 		241.2
(-2.6) 		401.8
(0.5) 		310

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 386
1 2 3 4 5 6 range
A 617.4
(2.6)
P 		642.5
(3)
P 		302.8
(-2.3)
P 		458.1
(0.1)
P 		257.3
(-3)
P 		361.8
(-1.4)
P 		385
B 405.8
(-0.3)
P 		536.7
(3)
P 		480
(1.6)
P 		358.1
(-1.5)
P 		296.8
(-3)
P 		366.3
(-1.3)
P 		240
C 487.6
(1.1)
P 		593.2
(3)
P 		478.9
(1)
P 		398.3
(-0.5)
P 		256.6
(-3)
P 		420.7
(-0.1)
P 		337
avr 503.6
(1.4) 		590.8
(3) 		420.6
(-0.2) 		404.8
(-0.5) 		270.2
(-3) 		382.9
(-0.9) 		321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 182.6
(3)
P 		61.2
(-2.8)
P 		65.7
(-2.6)
P 		58
(-3)
P 		125
B 193.8
(3)
P 		46.7
(-3)
P 		53.9
(-2.7)
P 		87.2
(-1.3)
P 		147
C 160.7
(3)
P 		55.3
(-3)
P 		83.4
(-1.4)
P 		67.4
(-2.3)
P 		105
avr 179
(3) 		54.4
(-3) 		67.7
(-2.4) 		70.9
(-2.2) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 113.8
(-1.2)
P 		228.5
(3)
P 		65.9
(-3)
P 		116.2
(-1.1)
P 		77.6
(-2.6)
P 		88.1
(-2.2)
P 		163
B 141.2
(0.3)
P 		178.7
(3)
P 		116.6
(-1.4)
P 		94.8
(-3)
P 		120.6
(-1.2)
P 		136.6
(-0)
P 		84
C 180.7
(3)
P 		181
(3)
P 		121.9
(-0.5)
P 		80.1
(-3)
P 		83
(-2.8)
P 		140.8
(0.6)
P 		101
avr 145.2
(0) 		196.1
(3) 		101.5
(-2.5) 		97
(-2.8) 		93.7
(-3) 		121.8
(-1.4) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172579_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5198
Sequence Type Exemplar sequence
Representative Public ID F59E12.6 NCBI
Target Description F59E12.6 /REP_DB=WormBase Gene ID /WP=CE11528 /TR=O01895 /GB=AAB54256.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062694(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062694 NCBI Caenorhabditis elegans F59E12.6a (F59E12.6) mRNA, complete cds. 11/11 None
SNAP00000018060 ENSEMBL cdna:SNAP chromosome:CEL140:II:5626402:5629413:-1 11/11 None
GENEFINDER00000018089 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5626402:5629413:-1 11/11 None
F59E12.6a ENSEMBL cdna:known chromosome:CEL140:II:5626278:5629672:-1 gene:F59E12.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182129 3/11 Cross Hyb Matching Probes None
NM_062646 3/11 Cross Hyb Matching Probes A 
NM_062657 7/11 Cross Hyb Matching Probes A 
SNAP00000012888 7/11 Cross Hyb Matching Probes None
GENEFINDER00000012892 7/11 Cross Hyb Matching Probes None
SNAP00000033522 2/11 Cross Hyb Matching Probes None
SNAP00000033523 2/11 Cross Hyb Matching Probes None
GENEFINDER00000033534 3/11 Cross Hyb Matching Probes None
F12A10.2 3/11 Cross Hyb Matching Probes A 
ZK177.2 7/11 Cross Hyb Matching Probes A 
F59E12.6b 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173951 2 1 NM_062694 172579_x_at (11), 187567_at (11), 187724_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5626399-5629129(-) 100.0 100.0
Public Domain and Genome References
Ensembl F59E12.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO38630 Hypothetical protein F59E12.6a [Caenorhabditis elegans] ref|NP_495095.2| F59E12.6a [Caenorhabditis elegans] 0.0
blast AAO38631 Hypothetical protein F59E12.6b [Caenorhabditis elegans] ref|NP_871929.1| F59E12.6b [Caenorhabditis elegans] 0.0
blast T15259 hypothetical protein F59E12.6 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.0E-8
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.6E-16
Sequence
Target Sequence 
>C. ELEGANS:172579_X_AT
tttggatcccaatttcggcatcggatgccctcccaagtgtgatgtagtgggaaacgaaaa
tgataccatctccacgcactccttgccacgcttccaacggttccgacctaaaatgatgcg
gaattccgaatgtgtcaaccatcttgcgatttttatgggttatggtggaacagttttggc
cgattggagaaccagcgactacagacggtgtatcacaattatggctcaattggttctggc
tatgaggattgccaacgacaagatgaaatttgttcatggtgacatctatatgatgaatat
tttaattgctccaacaaccaaacgatggatcgaatacaacattgatggcaaaacaattac
gattcaaacatttggcattatcccgcaattgattgacttttcgaaaagttggtgtggagc
cgatccggagcgtcacgacatcagtcgtttgcacacagtcgcgaaacgcgtcgggcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGGATCCCAATTTCGGCATCGGA 427 665 1164 Antisense
ATGCCCTCCCAAGTGTGATGTAGTG 583 39 1188 Antisense
ACGAAAATGATACCATCTCCACGCA 201 93 1217 Antisense
GAATGTGTCAACCATCTTGCGATTT 226 333 1291 Antisense
GGTGGAACAGTTTTGGCCGATTGGA 441 497 1327 Antisense
GAGAACCAGCGACTACAGACGGTGT 598 391 1350 Antisense
TATGGCTCAATTGGTTCTGGCTATG 9 655 1383 Antisense
AACATTTGGCATTATCCCGCAATTG 136 139 1530 Antisense
AAAGTTGGTGTGGAGCCGATCCGGA 251 123 1568 Antisense
ACATCAGTCGTTTGCACACAGTCGC 637 105 1601 Antisense
CACAGTCGCGAAACGCGTCGGGCAT 637 183 1617 Antisense
Sequence Source Affymetrix Proprietary Database