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[172578_x_at]

Description Y43F8B.2A /REP_DB=WormBase Gene ID /WP=CE21886 /TR=Q9XWX7 /GB=CAA21509.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 33      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 57      6grp_total > 11      4grp_total > 24      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2172
1 2 3 4 range
A 2477.4
(-3)
P 		2704
(-2.4)
P 		3389.4
(-0.5)
P 		4649.8
(3)
P 		2172
B 2502.4
(-3)
P 		3070.6
(-0.7)
P 		3974.3
(3)
P 		3880.3
(2.6)
P 		1472
C 2911
(-1.9)
P 		2730.4
(-3)
P 		3246
(0.1)
P 		3723.5
(3)
P 		993
avr 2630.3
(-3) 		2835
(-2.2) 		3536.6
(0.7) 		4084.5
(3) 		1454

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 951
1 2 3 4 5 6 range
A 2277.1
(-0.2)
P 		2781.9
(3)
P 		2764.9
(2.9)
P 		1831.1
(-3)
P 		2360
(0.3)
P 		2195.5
(-0.7)
P 		951
B 2252.2
(-1.1)
P 		2326.2
(-0.3)
P 		2164.8
(-2.1)
P 		2626.5
(3)
P 		2181.9
(-1.9)
P 		2079.7
(-3)
P 		547
C 2502.7
(3)
P 		2371.5
(1.6)
P 		2152
(-0.7)
P 		1939.4
(-3)
P 		2466.1
(2.6)
P 		2096.5
(-1.3)
P 		563
avr 2344
(0.6) 		2493.2
(3) 		2360.6
(0.8) 		2132.3
(-2.9) 		2336
(0.4) 		2123.9
(-3) 		369
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 544
1 2 3 4 range
A 902
(3)
P 		587
(-3)
P 		886
(2.7)
P 		680.6
(-1.2)
P 		315
B 798
(-1.5)
P 		758.4
(-3)
P 		920.9
(3)
P 		826.9
(-0.5)
P 		163
C 963.6
(1)
P 		785
(-1.1)
P 		1131.3
(3)
P 		627.8
(-3)
P 		504
avr 887.9
(1) 		710.1
(-3) 		979.4
(3) 		711.8
(-3) 		269

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 599
1 2 3 4 5 6 range
A 419.8
(-3)
P 		989.4
(3)
P 		602.1
(-1.1)
P 		464.4
(-2.5)
P 		712.2
(0.1)
P 		534.5
(-1.8)
P 		570
B 783.7
(1.3)
P 		774.3
(1.1)
P 		525.7
(-3)
P 		695.7
(-0.2)
P 		886.5
(3)
P 		775.5
(1.2)
P 		361
C 927.7
(3)
P 		723.6
(0.7)
P 		547.7
(-1.2)
P 		390
(-3)
P 		798
(1.6)
P 		701.8
(0.5)
P 		538
avr 710.4
(0.7) 		829.1
(3) 		558.5
(-2.2) 		516.7
(-3) 		798.9
(2.4) 		670.6
(-0) 		312
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172578_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14718
Sequence Type Exemplar sequence
Representative Public ID Y43F8B.2A NCBI
Target Description Y43F8B.2A /REP_DB=WormBase Gene ID /WP=CE21886 /TR=Q9XWX7 /GB=CAA21509.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075396(10), NM_075395(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075396 NCBI Caenorhabditis elegans Y43F8B.2b (Y43F8B.2) mRNA, complete cds. 10/11 A 
NM_075395 NCBI Caenorhabditis elegans Y43F8B.2a (Y43F8B.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000027196 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19522740:19524408:-1 10/11 None
Y43F8B.2b ENSEMBL cdna:known chromosome:CEL140:V:19522557:19525359:-1 gene:Y43F8B.2 10/11 A 
Y43F8B.2a ENSEMBL cdna:known chromosome:CEL140:V:19522740:19535682:-1 gene:Y43F8B.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19522739-19535682(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y43F8B.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21509 Hypothetical protein Y43F8B.2a [Caenorhabditis elegans] ref|NP_507796.1| Y43F8B.2a [Caenorhabditis elegans] 1.0E-169
blast CAA21510 Hypothetical protein Y43F8B.2b [Caenorhabditis elegans] ref|NP_507797.1| Y43F8B.2b [Caenorhabditis elegans] 1.0E-114
blast CAE61718 Hypothetical protein CBG05668 [Caenorhabditis briggsae] 1.0E-104
blast CAA21509 Hypothetical protein Y43F8B.2a [Caenorhabditis elegans] ref|NP_507796.1| Y43F8B.2a [Caenorhabditis elegans] 2.0E-88
blast CAA21510 Hypothetical protein Y43F8B.2b [Caenorhabditis elegans] ref|NP_507797.1| Y43F8B.2b [Caenorhabditis elegans] 3.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR007967 EMBL-EBI Protein of unknown function DUF727 5.2E-77
Pfam IPR007967 EMBL-EBI Protein of unknown function DUF727 5.2E-77
Pfam IPR007967 EMBL-EBI Protein of unknown function DUF727 5.2E-77
Sequence
Target Sequence 
>C. ELEGANS:172578_X_AT
acagagttctgtcccaatgtctcttcacaaggtatcatcaatcaaccggtcagcaggcac
caacccagcatcgagaggtggtgaatcgtcattggaactggaagcaattgctgccgtcca
cgaactttcgttcgccgtccaatccatctcagtgtcggagatgctcccacgcacgcctga
cctcatttttgtcaatgtcaccaccttagaagctcaaccatattgcctggagctcacatt
gaagggatggagaatcacaagtctcagatcggactgtatggtcggagatttcacgagatt
ggagcttttcacaaagtactacgatagcttgtaccttcttatggacgatatctcgccggg
atacagagagcggttcagtgagaagcttgtgcagaggttgaagcttattgaggccggcga
ggaagaccaggtggctccatgtgcatccctccagtctccatctctttccactgattcgtc
atcaaaagaatctcaatactcttcaactgaatcccttccaattgtgacaccagtcagcac
accagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGAGTTCTGTCCCAATGTCTCTT 145 109 387 Antisense
GTATCATCAATCAACCGGTCAGCAG 440 453 418 Antisense
ACTGGAAGCAATTGCTGCCGTCCAC 376 97 483 Antisense
CGTCCAATCCATCTCAGTGTCGGAG 469 245 522 Antisense
CACGCCTGACCTCATTTTTGTCAAT 47 201 558 Antisense
GTCAATGTCACCACCTTAGAAGCTC 272 459 577 Antisense
ATAGCTTGTACCTTCTTATGGACGA 293 21 710 Antisense
GAGGAAGACCAGGTGGCTCCATGTG 604 403 805 Antisense
TCTCTTTCCACTGATTCGTCATCAA 539 611 847 Antisense
AACTGAATCCCTTCCAATTGTGACA 202 143 891 Antisense
TTGTGACACCAGTCAGCACACCAGC 176 707 908 Antisense
Sequence Source Affymetrix Proprietary Database