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[172577_x_at]

Description F59E12.5 /REP_DB=WormBase Gene ID /WP=CE11526 /TR=O01894 /GB=AAB54255.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 83      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 45      6grp_total > 74      4grp_total > 32      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1169
1 2 3 4 range
A 1778
(-3)
P 		2123.5
(-0.5)
P 		2615.1
(3)
P 		2588.2
(2.8)
P 		837
B 2287.5
(-3)
P 		2585.3
(-0.3)
P 		2947.3
(3)
P 		2455.6
(-1.5)
P 		660
C 1828.6
(-3)
P 		1887
(-2.5)
P 		2531.9
(3)
P 		2491.7
(2.7)
P 		703
avr 1964.7
(-3) 		2198.6
(-1.1) 		2698.1
(3) 		2511.8
(1.5) 		733

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1668
1 2 3 4 5 6 range
A 1819.9
(-3)
P 		2167.5
(-0.2)
P 		2477.8
(2.2)
P 		2575
(3)
P 		2229.6
(0.3)
P 		2520.8
(2.6)
P 		755
B 1486.8
(-3)
P 		1875
(-1.6)
P 		2213.2
(-0.4)
P 		3155
(3)
P 		2257.4
(-0.2)
P 		2121.8
(-0.7)
P 		1668
C 1764.7
(-3)
P 		1958.5
(-1.5)
P 		2548.2
(3)
P 		2242.2
(0.7)
P 		2493.5
(2.6)
P 		2373.3
(1.7)
P 		784
avr 1690.5
(-3) 		2000.3
(-1.1) 		2413.1
(1.5) 		2657.4
(3) 		2326.8
(0.9) 		2338.6
(1) 		967
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 504
1 2 3 4 range
A 647.4
(2.3)
P 		461
(-1.4)
P 		683.6
(3)
P 		378.9
(-3)
P 		305
B 729.5
(3)
P 		638.5
(0.4)
P 		683
(1.6)
P 		523.3
(-3)
P 		206
C 605.3
(-0.6)
P 		542.5
(-1.4)
P 		882.4
(3)
P 		420.1
(-3)
P 		462
avr 660.7
(1.3) 		547.3
(-0.9) 		749.7
(3) 		440.8
(-3) 		309

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 582
1 2 3 4 5 6 range
A 335.5
(-3)
P 		770.9
(3)
P 		539.6
(-0.2)
P 		653.1
(1.4)
P 		672.8
(1.6)
P 		613.7
(0.8)
P 		435
B 517.3
(-3)
P 		624.1
(-1.4)
P 		537.4
(-2.7)
P 		835.7
(1.8)
P 		917.2
(3)
P 		791.2
(1.1)
P 		400
C 654.1
(0.4)
P 		597.6
(-0.5)
P 		648.6
(0.3)
P 		450.9
(-3)
P 		806.9
(3)
P 		794.5
(2.8)
P 		356
avr 502.3
(-3) 		664.2
(0.3) 		575.2
(-1.5) 		646.6
(-0.1) 		799
(3) 		733.1
(1.7) 		297
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172577_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5010
Sequence Type Exemplar sequence
Representative Public ID F59E12.5 NCBI
Target Description F59E12.5 /REP_DB=WormBase Gene ID /WP=CE11526 /TR=O01894 /GB=AAB54255.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062693(11), NM_062692(10), NM_062696(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062693 NCBI Caenorhabditis elegans F59E12.5a (F59E12.5) mRNA, complete cds. 11/11 A 
NM_062692 NCBI Caenorhabditis elegans F59E12.5b (F59E12.5) mRNA, complete cds. 10/11 A 
NM_062696 NCBI Caenorhabditis elegans F59E12.4a (F59E12.4) mRNA, complete cds. 9/11 A 
SNAP00000018059 ENSEMBL cdna:SNAP chromosome:CEL140:II:5622866:5624686:1 11/11 None
SNAP00000018062 ENSEMBL cdna:SNAP chromosome:CEL140:II:5631328:5633167:1 9/11 None
GENEFINDER00000018079 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5631328:5633167:1 9/11 None
GENEFINDER00000018081 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5622866:5624686:1 11/11 None
F59E12.5a.2 ENSEMBL cdna:known chromosome:CEL140:II:5622858:5624815:1 gene:F59E12.5 11/11 A 
F59E12.5a.1 ENSEMBL cdna:known chromosome:CEL140:II:5622862:5624815:1 gene:F59E12.5 11/11 A 
F59E12.5b ENSEMBL cdna:known chromosome:CEL140:II:5622866:5624771:1 gene:F59E12.5 10/11 A 
F59E12.4a ENSEMBL cdna:known chromosome:CEL140:II:5631328:5633233:1 gene:F59E12.4 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_077570 3/11 Cross Hyb Matching Probes A 
NM_062695 8/11 Cross Hyb Matching Probes A 
GENEFINDER00000010982 3/11 Cross Hyb Matching Probes None
SNAP00000010975 3/11 Cross Hyb Matching Probes None
F59E12.4b 8/11 Cross Hyb Matching Probes A 
C40H5.6 3/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173952 2 1 NM_062696 172577_x_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5622863-5624684(+) 100.0 100.0
chrII:5631325-5633165(+) 99.49 99.5
Public Domain and Genome References
Ensembl F59E12.5
F59E12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54255 Hypothetical protein F59E12.5a [Caenorhabditis elegans] ref|NP_495094.1| F59E12.5a [Caenorhabditis elegans] 0.0
blast AAK82899 Hypothetical protein F59E12.5b [Caenorhabditis elegans] ref|NP_495093.1| F59E12.5b [Caenorhabditis elegans] 0.0
blast AAK82897 Hypothetical protein F59E12.4a [Caenorhabditis elegans] ref|NP_495097.1| F59E12.4a [Caenorhabditis elegans] 0.0
blast AAK82898 Hypothetical protein F59E12.4b [Caenorhabditis elegans] ref|NP_495096.1| F59E12.4b [Caenorhabditis elegans] 0.0
blast T15260 hypothetical protein F59E12.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007716 EMBL-EBI NPL4, putative zinc binding 3.5E-54
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 2.7E-4
Pfam IPR007717 EMBL-EBI NPL4 1.0E-126
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.0E-4
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 2.7E-4
Sequence
Target Sequence 
>C. ELEGANS:172577_X_AT
acggacgaccacttccagttgaatatttgctggtggatgttcctgctggaatgccaaaag
agcctcactacacgttccacgttggcacatctaataaatcgaagagtgccaaatttaatg
ttgaaaatcgacaagcaattggacaacttcaaggaggtgcaaatttgattcaatattcat
ccgaattctctacaaatcaatttttggaacaggctacaaacttccactttttgctctatt
tagtaaccaatgaccaagtgcagatttctgatgaatggatgaaacgattgtgtgatgcag
ttaaagctcaggacagaggaactgcaatggaatgggctcaggaatgtgaagattggcatc
agttgatggctttagcgcatgctaatggaggaagcggcaatgacgatattcctgtcattc
caaatggtgatcctttctccggatcaagcagtggtggatcaagaggagccgtttggagct
gtggacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGACGACCACTTCCAGTTGAATA 12 93 1043 Antisense
TTTGCTGGTGGATGTTCCTGCTGGA 387 667 1068 Antisense
GCCAAAAGAGCCTCACTACACGTTC 212 279 1095 Antisense
CACGTTCCACGTTGGCACATCTAAT 645 199 1113 Antisense
TATTCATCCGAATTCTCTACAAATC 671 663 1216 Antisense
GAACAGGCTACAAACTTCCACTTTT 335 337 1249 Antisense
AACTTCCACTTTTTGCTCTATTTAG 43 139 1261 Antisense
GACGATATTCCTGTCATTCCAAATG 36 379 1444 Antisense
ATTCCAAATGGTGATCCTTTCTCCG 618 7 1459 Antisense
TCCTTTCTCCGGATCAAGCAGTGGT 55 591 1473 Antisense
AGGAGCCGTTTGGAGCTGTGGACAC 27 57 1506 Antisense
Sequence Source Affymetrix Proprietary Database