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[172576_x_at]

Description F36H12.5 /REP_DB=WormBase Gene ID /WP=CE09977 /TR=O76715 /GB=AAC26931.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 48      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 13.7
(-3)
A 		24.5
(-1.6)
A 		60.9
(3)
A 		22
(-1.9)
A 		47
B 11.5
(-1.6)
A 		42.7
(3)
A 		36.4
(2.1)
A 		1.7
(-3)
A 		41
C 8.5
(-3)
A 		33.8
(2.4)
A 		19.8
(-0.6)
A 		36.4
(3)
A 		28
avr 11.2
(-3) 		33.7
(1.9) 		39
(3) 		20
(-1.1) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 5.4
(-3)
A 		49.7
(-0.5)
A 		41.2
(-1)
A 		41.2
(-1)
A 		89.7
(1.8)
A 		110.5
(3)
P 		105
B 34.7
(-1.9)
A 		10.5
(-3)
A 		19.2
(-2.6)
A 		9.5
(-3)
A 		104.6
(1.1)
A 		149.4
(3)
P 		140
C 22
(-3)
A 		36.9
(-2.3)
A 		37.7
(-2.3)
A 		58.3
(-1.4)
A 		89.5
(0)
M 		156.9
(3)
P 		135
avr 20.7
(-3) 		32.4
(-2.4) 		32.7
(-2.4) 		36.3
(-2.2) 		94.6
(0.8) 		138.9
(3) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 5
(-2.1)
A 		5.3
(-2)
A 		15.9
(3)
A 		3.2
(-3)
A 		13
B 3.7
(-1)
A 		10.5
(3)
A 		8.4
(1.8)
A 		0.4
(-3)
A 		10
C 2.8
(-3)
A 		9.7
(3)
A 		6.9
(0.6)
A 		6.1
(-0.1)
A 		7
avr 3.8
(-2.5) 		8.5
(1.4) 		10.4
(3) 		3.2
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 1
(-3)
A 		17.7
(0.8)
A 		9
(-1.2)
A 		10.5
(-0.8)
A 		27.1
(3)
A 		26.9
(3)
P 		26
B 12.1
(-1.9)
A 		3.5
(-2.9)
A 		4.7
(-2.8)
A 		2.5
(-3)
A 		42.5
(1.5)
A 		55.7
(3)
P 		53
C 8.1
(-3)
A 		11.3
(-2.6)
A 		9.6
(-2.8)
A 		11.7
(-2.5)
A 		29
(-0.2)
M 		52.5
(3)
P 		44
avr 7.1
(-3) 		10.8
(-2.4) 		7.8
(-2.9) 		8.2
(-2.8) 		32.9
(1.1) 		45
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172576_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11092
Sequence Type Exemplar sequence
Representative Public ID F36H12.5 NCBI
Target Description F36H12.5 /REP_DB=WormBase Gene ID /WP=CE09977 /TR=O76715 /GB=AAC26931.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068362(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068362 NCBI Caenorhabditis elegans F36H12.5 (F36H12.5) mRNA, complete cds. 11/11 None
SNAP00000002642 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5266855:5267557:1 11/11 None
GENEFINDER00000002655 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5266855:5267557:1 11/11 None
F36H12.5 ENSEMBL cdna:known chromosome:CEL140:IV:5266855:5267557:1 gene:F36H12.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068552 1/11 Cross Hyb Matching Probes A 
SNAP00000039095 1/11 Cross Hyb Matching Probes None
GENEFINDER00000039106 1/11 Cross Hyb Matching Probes None
T13A10.1 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5266860-5267563(+) 79.46 100.0
chrIV:6276383-6277088(+) 50.17 92.09
Public Domain and Genome References
Gene Title
Ensembl F36H12.5
Entrez Gene 185395 Entrez gene
SwissProt O76715 EMBL-EBI
WormBase CE09977 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26931 Hypothetical protein F36H12.5 [Caenorhabditis elegans] ref|NP_500763.1| F36H12.5 [Caenorhabditis elegans] 7.0E-29
blast NP_500953 T13A10.1 [Caenorhabditis elegans] gb|AAB38121.1| Hypothetical protein T13A10.1 [Caenorhabditis elegans] 2.0E-20
blast CAE65005 Hypothetical protein CBG09843 [Caenorhabditis briggsae] 5.0E-17
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500763.1 1 5-24
F36H12.5 1 5-24
SNAP00000002642 1 5-24
GENEFINDER00000002655 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:172576_X_AT
gattcatttggtttgtgcttatcacattttcgcgataatactaatgtcgatgactgtctc
tgttggatgcaaatcgaaggctaaaaaaccaaaagcagctgctccgaacccgataagctc
gatctcgtcaaactcgaaagtcgcccctccagcttctccaccaccaccaccagaagctga
aaagaagccggaaacgggagaagagccgaagaaagagaaatcgaagatgaaaagtgaagt
gaaggaaccagatgctccaaaaaaggatgacaaaacatcagatgaaaagaaagagaaaca
ggaggatacatttgatgctgtcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCATTTGGTTTGTGCTTATCAC 472 431 15 Antisense
ATCACATTTTCGCGATAATACTAAT 212 27 35 Antisense
ATACTAATGTCGATGACTGTCTCTG 126 25 52 Antisense
GACTGTCTCTGTTGGATGCAAATCG 683 375 66 Antisense
AAACCAAAAGCAGCTGCTCCGAACC 477 125 100 Antisense
CGAACCCGATAAGCTCGATCTCGTC 546 251 119 Antisense
TCTCGTCAAACTCGAAAGTCGCCCC 629 609 137 Antisense
ACCACCACCACCAGAAGCTGAAAAG 596 85 174 Antisense
GAAGCCGGAAACGGGAGAAGAGCCG 477 343 198 Antisense
GAAGTGAAGGAACCAGATGCTCCAA 209 335 250 Antisense
AGGAGGATACATTTGATGCTGTCAA 583 55 314 Antisense
Sequence Source Affymetrix Proprietary Database