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[172571_x_at]

Description H12I19.5A /REP_DB=WormBase Gene ID /WP=CE16177 /TR=O45621 /GB=CAB11542.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 23.3
(-3)
A 		29.3
(-2.1)
A 		46.8
(0.7)
A 		61.2
(3)
A 		38
B 42.9
(2.4)
A 		42.6
(2.3)
A 		44.9
(3)
A 		24.1
(-3)
A 		21
C 73
(-0.1)
P 		36.8
(-2.7)
A 		32
(-3)
A 		116.1
(3)
P 		84
avr 46.4
(-1) 		36.2
(-3) 		41.2
(-2) 		67.1
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 21.6
(-1.7)
A 		12.3
(-3)
A 		43.2
(1.2)
P 		56.9
(3)
A 		30.3
(-0.6)
A 		34.6
(0)
A 		45
B 14.3
(-3)
A 		25
(-1)
A 		30.8
(0)
A 		47.1
(3)
P 		38.4
(1.4)
P 		38.1
(1.4)
A 		33
C 29.3
(-0.7)
A 		67.2
(3)
P 		6.4
(-3)
A 		52.7
(1.6)
P 		49.5
(1.3)
A 		28.7
(-0.8)
A 		61
avr 21.7
(-3) 		34.8
(-0.4) 		26.8
(-2) 		52.2
(3) 		39.4
(0.5) 		33.8
(-0.6) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 8.5
(-0.8)
A 		6.4
(-3)
A 		12.2
(3)
A 		9
(-0.3)
A 		6
B 13.7
(3)
A 		10.5
(0.8)
A 		10.4
(0.7)
A 		5.1
(-3)
A 		9
C 24.2
(3)
P 		10.6
(-3)
A 		11.2
(-2.7)
A 		19.6
(1)
P 		14
avr 15.5
(3) 		9.2
(-3) 		11.3
(-1) 		11.2
(-1.1) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 4
(-3)
A 		4.4
(-2.8)
A 		9.4
(0.1)
P 		14.4
(3)
A 		9.1
(-0.1)
A 		8.4
(-0.5)
A 		10
B 5
(-3)
A 		8.3
(-1.1)
A 		7.5
(-1.6)
A 		12.5
(1.2)
P 		15.6
(3)
P 		14.2
(2.2)
A 		11
C 10.9
(-0)
A 		20.5
(3)
P 		1.6
(-3)
A 		10.6
(-0.1)
P 		16
(1.6)
A 		9.6
(-0.5)
A 		19
avr 6.6
(-2.7) 		11.1
(1) 		6.2
(-3) 		12.5
(2.1) 		13.6
(3) 		10.7
(0.6) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172571_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9347
Sequence Type Exemplar sequence
Representative Public ID H12I19.5A NCBI
Target Description H12I19.5A /REP_DB=WormBase Gene ID /WP=CE16177 /TR=O45621 /GB=CAB11542.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070311(10), NM_070312(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070311 NCBI Caenorhabditis elegans H12I19.5b (H12I19.5) mRNA, complete cds. 10/11 A 
NM_070312 NCBI Caenorhabditis elegans H12I19.5a (H12I19.5) mRNA, complete cds. 11/11 A 
GENEFINDER00000013881 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:14148898:14153924:-1 11/11 None
H12I19.5c ENSEMBL cdna:known chromosome:CEL140:IV:14150261:14153296:-1 gene:H12I19.5 11/11 A 
H12I19.5b ENSEMBL cdna:known chromosome:CEL140:IV:14150290:14153296:-1 gene:H12I19.5 10/11 A 
H12I19.5a ENSEMBL cdna:known chromosome:CEL140:IV:14150300:14153330:-1 gene:H12I19.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14150503-14153302(-) 100.0 100.0
Public Domain and Genome References
Ensembl H12I19.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB11541 Hypothetical protein H12I19.5b [Caenorhabditis elegans] ref|NP_502712.1| H12I19.5b [Caenorhabditis elegans] 0.0
blast CAB11542 Hypothetical protein H12I19.5a [Caenorhabditis elegans] ref|NP_502713.1| H12I19.5a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.0E-126
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 3.6E-108
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502712.1 7 28-50,77-96,139-161,168-187,244-266,287-309,319-341
NP_502713.1 7 28-50,77-96,139-161,168-187,244-266,287-309,319-341
H12I19.5c 8 28-50,77-96,139-161,168-187,244-266,287-309,319-341,353-370
H12I19.5b 7 28-50,77-96,139-161,168-187,244-266,287-309,319-341
H12I19.5a 7 28-50,77-96,139-161,168-187,244-266,287-309,319-341
GENEFINDER00000013881 7 39-61,97-116,159-181,188-207,264-286,307-329,339-361
Sequence
Target Sequence 
>C. ELEGANS:172571_X_AT
gagagccttcatcggaaatggtccatttatgattatcgaaacttttatgcgccatcatgt
gattatggggatggtgcaggaccacttggttggtgtgagcacactggtctcgataagaga
aacggtaaatttaaatttcttctcattggaaattgttgggcagctaatcatgctagaata
ttttacgatgaatgtaaagtgcaggcaaaacgtatcgtacaattctcggaggcaggtgag
gcacagcctagaattttgaaccatatttttttggaatttatattattttgctgttgtgac
ccactggtttctcatagatataatgttgaatactgtgttcccgctctagatcggtacgcg
gaacaggttgaaaaagaaaaaccagattacctatttttaatgtcaagaatgtttgacatt
ggagacccgattcctcaaaatgcaacagttctaggttatgatcctatatctcagtcaatg
aaaaatcaattaaacaaaatggttaaacatgtcaaaaaaaagatttatattctgacgtca
ttgccggcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGCCTTCATCGGAAATGGTCCA 268 395 1213 Antisense
TATCGAAACTTTTATGCGCCATCAT 200 653 1246 Antisense
GGTGCAGGACCACTTGGTTGGTGTG 614 499 1285 Antisense
GGTGTGAGCACACTGGTCTCGATAA 592 499 1304 Antisense
GTATCGTACAATTCTCGGAGGCAGG 48 453 1424 Antisense
TTATTTTGCTGTTGTGACCCACTGG 60 679 1495 Antisense
GTGACCCACTGGTTTCTCATAGATA 383 483 1508 Antisense
AATGTTGAATACTGTGTTCCCGCTC 138 165 1534 Antisense
TTCCCGCTCTAGATCGGTACGCGGA 58 697 1550 Antisense
TGACATTGGAGACCCGATTCCTCAA 295 571 1626 Antisense
ATATTCTGACGTCATTGCCGGCAAT 462 19 1739 Antisense
Sequence Source Affymetrix Proprietary Database