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[172547_x_at]

Description R12E2.14 /REP_DB=WormBase Gene ID /WP=CE18146 /TR=O61782 /GB=AAC17035.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 33      4grp_highest_8_8 > 02      4grp_high_8_8 > 54      6grp_highest_nonorm > 24      6grp_total > 10      4grp_total > 57      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8811
1 2 3 4 range
A 126.4
(-3)
P 		6075.9
(1.1)
P 		8761
(3)
P 		319.3
(-2.9)
P 		8635
B 148.3
(-3)
P 		7464.2
(2)
P 		8937.8
(3)
P 		214.5
(-3)
P 		8790
C 157.3
(-3)
P 		8838.1
(3)
P 		7865.9
(2.3)
P 		193.7
(-3)
P 		8681
avr 144
(-3) 		7459.4
(2.2) 		8521.6
(3) 		242.5
(-2.9) 		8378

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3558
1 2 3 4 5 6 range
A 2441.6
(-1.2)
P 		4358
(3)
P 		4059.5
(2.4)
P 		1940.7
(-2.2)
P 		2434.6
(-1.2)
P 		1591.5
(-3)
P 		2767
B 2556.2
(-1.7)
P 		4889.2
(3)
P 		3909.1
(1)
P 		2236.9
(-2.3)
P 		2534.1
(-1.7)
P 		1898.6
(-3)
P 		2991
C 2183.1
(-2.5)
P 		5149.2
(3)
P 		2390.5
(-2.1)
P 		2833
(-1.3)
P 		2310.8
(-2.2)
P 		1904.1
(-3)
P 		3245
avr 2393.6
(-1.8) 		4798.8
(3) 		3453
(0.3) 		2336.9
(-1.9) 		2426.5
(-1.7) 		1798.1
(-3) 		3001
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2709
1 2 3 4 range
A 46
(-3)
P 		1318.9
(0.4)
P 		2290.3
(3)
P 		46.7
(-3)
P 		2244
B 47.3
(-3)
P 		1843.5
(2.3)
P 		2071.1
(3)
P 		45.7
(-3)
P 		2025
C 52.1
(-3)
P 		2541
(2.6)
P 		2741.5
(3)
P 		32.7
(-3)
P 		2709
avr 48.5
(-3) 		1901.1
(1.8) 		2367.6
(3) 		41.7
(-3) 		2326

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1240
1 2 3 4 5 6 range
A 450.1
(-2.7)
P 		1549.9
(3)
P 		884.1
(-0.4)
P 		492.2
(-2.5)
P 		734.7
(-1.2)
P 		387.5
(-3)
P 		1162
B 889.4
(-1.3)
P 		1627.4
(3)
P 		949.3
(-0.9)
P 		592.5
(-3)
P 		1029.6
(-0.5)
P 		708
(-2.3)
P 		1035
C 809.2
(-1.6)
P 		1571.2
(3)
P 		608.4
(-2.8)
P 		569.7
(-3)
P 		747.7
(-1.9)
P 		637.4
(-2.6)
P 		1002
avr 716.2
(-2) 		1582.8
(3) 		813.9
(-1.5) 		551.5
(-3) 		837.3
(-1.3) 		577.6
(-2.8) 		1031
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172547_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2521
Sequence Type Exemplar sequence
Representative Public ID R12E2.14 NCBI
Target Description R12E2.14 /REP_DB=WormBase Gene ID /WP=CE18146 /TR=O61782 /GB=AAC17035.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058908(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058908 NCBI Caenorhabditis elegans R12E2.14 (R12E2.14) mRNA, complete cds. 11/11 A 
GENEFINDER00000012699 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4149878:4150563:1 11/11 None
SNAP00000012674 ENSEMBL cdna:SNAP chromosome:CEL140:I:4150001:4150563:1 11/11 None
R12E2.14 ENSEMBL cdna:known chromosome:CEL140:I:4150001:4150750:1 gene:R12E2.14 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027854 1/11 Cross Hyb Matching Probes None
NM_058907 1/11 Cross Hyb Matching Probes A 
SNAP00000012673 1/11 Cross Hyb Matching Probes None
GENEFINDER00000012698 1/11 Cross Hyb Matching Probes None
SNAP00000011222 1/11 Cross Hyb Matching Probes None
GENEFINDER00000011235 1/11 Cross Hyb Matching Probes None
R12E2.15 1/11 Cross Hyb Matching Probes A 
ZK643.8 1/11 Cross Hyb Matching Probes A B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4150000-4150563(+) 100.0 100.0
chrI:4148941-4149412(-) 92.44 92.44
Public Domain and Genome References
Gene Title
Ensembl R12E2.14
Entrez Gene 172001 Entrez gene
SwissProt O61782 EMBL-EBI
WormBase CE18146 Wormbase
Sequence
Target Sequence 
>C. ELEGANS:172547_X_AT
cctctggaaacgcctgtctcggaggtggaggtggtgccggtggatgctgcccaccagctc
agccaggatgtggaaacccatgccaaggagccgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTCTGGAAACGCCTGTCTCGGAGG 486 267 56 Antisense
AAACGCCTGTCTCGGAGGTGGAGGT 460 123 63 Antisense
CGCCTGTCTCGGAGGTGGAGGTGGT 123 255 66 Antisense
GTCTCGGAGGTGGAGGTGGTGCCGG 663 469 71 Antisense
CGGAGGTGGAGGTGGTGCCGGTGGA 536 241 75 Antisense
CCCACCAGCTCAGCCAGGATGTGGA 568 257 105 Antisense
ACCAGCTCAGCCAGGATGTGGAAAC 643 85 108 Antisense
GCTCAGCCAGGATGTGGAAACCCAT 255 301 112 Antisense
GCCAGGATGTGGAAACCCATGCCAA 258 281 117 Antisense
GGATGTGGAAACCCATGCCAAGGAG 609 511 121 Antisense
TGGAAACCCATGCCAAGGAGCCGGA 534 545 126 Antisense
Sequence Source Affymetrix Proprietary Database