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[172543_x_at]

Description F35F10.4 /REP_DB=WormBase Gene ID /WP=CE25871 /TR=Q9GYU0 /GB=AAF99939.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 52      4grp_high_8_8 > 60      6grp_total > 62      4grp_total > 45      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 47.2
(2.1)
P 		51.4
(3)
P 		24.1
(-3)
A 		46.7
(2)
A 		27
B 43.3
(0.9)
A 		53.1
(3)
A 		34.1
(-1.1)
A 		25.4
(-3)
P 		28
C 56.7
(1.4)
P 		34.8
(-3)
A 		64.9
(3)
P 		47.6
(-0.4)
A 		30
avr 49.1
(3) 		46.4
(1.2) 		41
(-2.3) 		39.9
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 13
(-3)
A 		50.3
(-0.6)
P 		80.1
(1.3)
P 		105.9
(3)
P 		34.3
(-1.6)
A 		39.3
(-1.3)
P 		93
B 35.3
(-3)
P 		83.5
(1.2)
P 		104.1
(3)
P 		82.8
(1.1)
P 		45.5
(-2.1)
P 		49.4
(-1.8)
P 		69
C 15
(-3)
A 		66.5
(0.6)
P 		101
(3)
P 		78.9
(1.5)
P 		45.4
(-0.9)
P 		33.1
(-1.7)
A 		86
avr 21.1
(-3) 		66.8
(0.7) 		95.1
(3) 		89.2
(2.5) 		41.7
(-1.3) 		40.6
(-1.4) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 17.2
(3)
P 		11.2
(-0.3)
P 		6.3
(-3)
A 		6.8
(-2.7)
A 		11
B 13.8
(3)
A 		13.1
(2.5)
A 		7.9
(-1.2)
A 		5.4
(-3)
P 		8
C 18.8
(1.4)
P 		10
(-2.2)
A 		22.6
(3)
P 		8
(-3)
A 		15
avr 16.6
(3) 		11.4
(-0.2) 		12.3
(0.4) 		6.7
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 2.4
(-3)
A 		17.9
(0.8)
P 		17.4
(0.7)
P 		26.9
(3)
P 		10.3
(-1.1)
A 		9.6
(-1.2)
P 		25
B 12.3
(-3)
P 		27.8
(3)
P 		25.3
(2)
P 		21.9
(0.7)
P 		18.5
(-0.6)
P 		18.4
(-0.6)
P 		16
C 5.6
(-3)
A 		20.3
(1.4)
P 		25.7
(3)
P 		15.9
(0.1)
P 		14.7
(-0.3)
P 		11.1
(-1.4)
A 		20
avr 6.8
(-3) 		22
(2.7) 		22.8
(3) 		21.6
(2.6) 		14.5
(-0.1) 		13
(-0.7) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172543_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15354
Sequence Type Exemplar sequence
Representative Public ID F35F10.4 NCBI
Target Description F35F10.4 /REP_DB=WormBase Gene ID /WP=CE25871 /TR=Q9GYU0 /GB=AAF99939.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071510(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071510 NCBI Caenorhabditis elegans F35F10.4 (F35F10.4) mRNA, complete cds. 9/11 None
GENEFINDER00000011261 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3289835:3294895:1 11/11 None
F35F10.4 ENSEMBL cdna:known chromosome:CEL140:V:3290661:3294949:1 gene:F35F10.4 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071537 2/11 Cross Hyb Matching Probes A 
NM_071534 1/11 Cross Hyb Matching Probes None
NM_071535 2/11 Cross Hyb Matching Probes A B 
SNAP00000011242 2/11 Cross Hyb Matching Probes None
SNAP00000011243 7/11 Cross Hyb Matching Probes None
SNAP00000029748 1/11 Cross Hyb Matching Probes None
SNAP00000029750 1/11 Cross Hyb Matching Probes None
SNAP00000029751 2/11 Cross Hyb Matching Probes None
GENEFINDER00000029761 1/11 Cross Hyb Matching Probes None
GENEFINDER00000029765 2/11 Cross Hyb Matching Probes None
C04E12.5 1/11 Cross Hyb Matching Probes A 
C04E12.4 2/11 Cross Hyb Matching Probes A B 
C04E12.2 2/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3289834-3294895(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35F10.4
Entrez Gene 178762 Entrez gene
SwissProt Q9GYU0 EMBL-EBI
WormBase CE35303 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99939 Hypothetical protein F35F10.4 [Caenorhabditis elegans] ref|NP_503911.4| F35F10.4 [Caenorhabditis elegans] 0.0
blast AAC27091 Hypothetical protein C04E12.5 [Caenorhabditis elegans] ref|NP_503935.2| C04E12.5 [Caenorhabditis elegans] 0.0
blast T15149 hypothetical protein F35F10.3 - Caenorhabditis elegans 0.0
blast AAC27089 Hypothetical protein C04E12.4 [Caenorhabditis elegans] ref|NP_503936.2| C04E12.4 [Caenorhabditis elegans] 1.0E-163
blast AAC27091 Hypothetical protein C04E12.5 [Caenorhabditis elegans] ref|NP_503935.2| C04E12.5 [Caenorhabditis elegans] 1.0E-125
blast AAC27089 Hypothetical protein C04E12.4 [Caenorhabditis elegans] ref|NP_503936.2| C04E12.4 [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 4.1E-46
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.8E-45
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 4.1E-46
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 7.8E-61
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000011261 1 43-65
Sequence
Target Sequence 
>C. ELEGANS:172543_X_AT
atggatgagattgccgataatgacggaacagccaatgcttgtctacacaagtgcacggct
attccgataaacggaagcttgaccattgagaagccccagtttaataagtttttcatgcaa
attgaattgctcaaaaatataaaagtattcaaaactgaacttaacaatagaagcagtgtc
gaaagtatttctattgttgtctactggaacataaagaaggcaagcagtttgttggaattc
agcaaatcatgcaatgccatgcaaaactgtcttgatgctctgaggtgtggacagggaact
gaacatcttgcgatgtacgaaaaaatgagagggcaatgtgtgaccatgcacttcttttca
acagacgttttctcttgtctgactaagttagaaaattcgaagccgaaacctgaatgctat
gagaaatgggacactaaattagtcaacgaagatttaacagaagtgagcccagacaaattg
tgtaagaaaatgtttggaaaagacaattgtatgaagaaagtagtcaaggaaacttgtgga
gacaaagattggcagctacttctgcaacatttcaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGATGAGATTGCCGATAATGACG 58 53 1252 Antisense
GGAACAGCCAATGCTTGTCTACACA 319 529 1276 Antisense
CTACACAAGTGCACGGCTATTCCGA 340 225 1294 Antisense
TTGACCATTGAGAAGCCCCAGTTTA 403 699 1330 Antisense
AACTGTCTTGATGCTCTGAGGTGTG 293 143 1516 Antisense
GACAGGGAACTGAACATCTTGCGAT 176 369 1541 Antisense
GAGAGGGCAATGTGTGACCATGCAC 62 393 1578 Antisense
GACCATGCACTTCTTTTCAACAGAC 309 383 1593 Antisense
TTTTCAACAGACGTTTTCTCTTGTC 77 677 1606 Antisense
CAGAAGTGAGCCCAGACAAATTGTG 491 203 1709 Antisense
CAGCTACTTCTGCAACATTTCAATG 108 205 1804 Antisense
Sequence Source Affymetrix Proprietary Database