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[172540_x_at]

Description C44E12.3 /REP_DB=WormBase Gene ID /WP=CE02550 /TR=Q18623 /GB=AAA80376.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 07      6grp_total > 18      4grp_total > 29      6grp_cgc > 01      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 146.7
(-0.1)
P 		72.5
(-3)
A 		73.3
(-3)
A 		224.9
(3)
M 		152
B 143.1
(1.2)
P 		162.1
(3)
P 		97.6
(-3)
P 		129.4
(-0)
M 		65
C 195.3
(3)
P 		135.7
(-0.6)
P 		94.6
(-3)
P 		131.5
(-0.8)
M 		101
avr 161.7
(3) 		123.4
(-0.1) 		88.5
(-3) 		161.9
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 187.2
(3)
P 		140.3
(0.2)
P 		113.3
(-1.4)
P 		85.9
(-3)
P 		128
(-0.5)
P 		98.7
(-2.2)
P 		101
B 164.9
(3)
P 		122.5
(-0.1)
P 		91.4
(-2.3)
A 		114.6
(-0.7)
M 		105.1
(-1.3)
P 		82.4
(-3)
P 		83
C 188.6
(3)
P 		131.3
(-0.6)
P 		104
(-2.3)
P 		157.9
(1.1)
P 		123.1
(-1.1)
P 		92.9
(-3)
P 		96
avr 180.2
(3) 		131.4
(-0.3) 		102.9
(-2.2) 		119.5
(-1.1) 		118.7
(-1.2) 		91.3
(-3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 53.4
(3)
P 		15.7
(-3)
A 		19.2
(-2.4)
A 		32.9
(-0.3)
M 		38
B 45.6
(3)
P 		40
(1.5)
P 		22.6
(-3)
P 		27.6
(-1.7)
M 		23
C 64.7
(3)
P 		39
(-0.6)
P 		33
(-1.5)
P 		22.2
(-3)
M 		43
avr 54.6
(3) 		31.6
(-1.6) 		24.9
(-3) 		27.6
(-2.5) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 34.5
(-0.3)
P 		49.9
(3)
P 		24.7
(-2.4)
P 		21.8
(-3)
P 		38.6
(0.6)
P 		24
(-2.5)
P 		28
B 57.4
(3)
P 		40.8
(0.2)
P 		22.2
(-3)
A 		30.4
(-1.6)
M 		42.7
(0.5)
P 		30.7
(-1.6)
P 		35
C 69.9
(3)
P 		40.1
(-1.1)
P 		26.5
(-3)
P 		31.8
(-2.3)
P 		39.8
(-1.2)
P 		31.1
(-2.4)
P 		43
avr 53.9
(3) 		43.6
(0.9) 		24.5
(-3) 		28
(-2.3) 		40.4
(0.2) 		28.6
(-2.2) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172540_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16022
Sequence Type Exemplar sequence
Representative Public ID C44E12.3 NCBI
Target Description C44E12.3 /REP_DB=WormBase Gene ID /WP=CE02550 /TR=Q18623 /GB=AAA80376.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029294(9), NM_001029296(10), NM_001029292(9), NM_001029293(9), NM_001029291(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029294 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-17) (twk-17) mRNA, complete cds. 9/11 None
NM_001029296 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-17) (twk-17) mRNA, complete cds. 10/11 None
NM_001029292 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-17) (twk-17) mRNA, complete cds. 9/11 None
NM_001029293 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-17) (twk-17) mRNA, complete cds. 9/11 None
NM_001029291 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-17) (twk-17) mRNA, complete cds. 9/11 None
SNAP00000016789 ENSEMBL cdna:SNAP chromosome:CEL140:X:7600307:7603348:-1 9/11 None
GENEFINDER00000016791 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7600307:7606584:-1 10/11 None
C44E12.3d ENSEMBL cdna:known chromosome:CEL140:X:7599778:7606338:-1 gene:C44E12.3 9/11 A 
C44E12.3e ENSEMBL cdna:known chromosome:CEL140:X:7599810:7606338:-1 gene:C44E12.3 9/11 A 
C44E12.3f ENSEMBL cdna:known chromosome:CEL140:X:7600307:7607948:-1 gene:C44E12.3 10/11 None
C44E12.3c ENSEMBL cdna:known chromosome:CEL140:X:7600307:7606338:-1 gene:C44E12.3 9/11 A 
C44E12.3a ENSEMBL cdna:known chromosome:CEL140:X:7600307:7604350:-1 gene:C44E12.3 9/11 A 
C44E12.3b ENSEMBL cdna:known chromosome:CEL140:X:7600307:7607948:-1 gene:C44E12.3 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029295 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181024 6 5 NM_001029291 172540_x_at (9)
NM_001029292 172540_x_at (9)
NM_001029293 172540_x_at (9)
NM_001029294 172540_x_at (9)
NM_001029296 172540_x_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7600305-7604349(-) 100.0 100.0
Public Domain and Genome References
Ensembl C44E12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM51532 Twik family of potassium channels protein 17, isoform d [Caenorhabditis elegans] ref|NP_001024465.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] gb|AAC32862.1| putative potassium channel subunit n2P17m2-1 [Caenorhabditis elegans] 0.0
blast AAM51531 Twik family of potassium channels protein 17, isoform c [Caenorhabditis elegans] ref|NP_001024464.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] gb|AAC32858.1| putative potassium channel subunit n2P17m2-2 [Caenorhabditis elegans] 0.0
blast AAP31441 Twik family of potassium channels protein 17, isoform e [Caenorhabditis elegans] ref|NP_001024466.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] gb|AAC32859.1| putative potassium channel subunit n2P17m2-3 [Caenorhabditis elegans] 0.0
blast AAW30673 Twik family of potassium channels protein 17, isoform f [Caenorhabditis elegans] ref|NP_001024467.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] gb|AAC32854.1| putative potassium channel subunit n2P17m1-2 [Caenorhabditis elegans] 0.0
blast AAM51530 Twik family of potassium channels protein 17, isoform b [Caenorhabditis elegans] ref|NP_001024463.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] gb|AAC32855.1| putative potassium channel subunit n2P17m1-1 [Caenorhabditis elegans] 0.0
blast AAM51529 Twik family of potassium channels protein 17, isoform a [Caenorhabditis elegans] ref|NP_001024462.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024465.1 6 142-164,243-265,275-297,365-387,392-414,421-440
NP_001024467.1 6 78-100,179-201,211-233,301-323,328-350,357-376
NP_001024463.1 6 78-100,179-201,211-233,301-323,328-350,357-376
NP_001024464.1 6 142-164,243-265,275-297,365-387,392-414,421-440
NP_001024462.1 6 85-107,186-208,218-240,308-330,335-357,364-383
C44E12.3d 6 142-164,243-265,275-297,365-387,392-414,421-440
C44E12.3e 6 142-164,243-265,275-297,365-387,392-414,421-440
C44E12.3f 6 78-100,179-201,211-233,301-323,328-350,357-376
C44E12.3c 6 142-164,243-265,275-297,365-387,392-414,421-440
C44E12.3a 6 85-107,186-208,218-240,308-330,335-357,364-383
C44E12.3b 6 78-100,179-201,211-233,301-323,328-350,357-376
SNAP00000016789 5 88-110,125-147,176-198,203-225,232-251
GENEFINDER00000016791 6 224-246,325-347,357-379,447-469,474-496,595-617
Sequence
Target Sequence 
>C. ELEGANS:172540_X_AT
agcacgtctttgatatctattcccaaattatgaatgaaactattgcccttacaaataatc
aaagtgatcaagcaacgattgttagtcggatgagaccattacttgagtcattatcaagag
ctcacgagtacgatgataaattcactgatacaaatcaattatggacaggtgagcaggatg
ggatgacgacaagatggacatttgctgcagctacactttatgcacttactgtgataactt
ctaccgctcgcggtatcttatgtaattatcggtcttgctgtcatcactgcatcactggat
ctctgttcctctacactgaaacgaacatttaccaaacttcactactttggaagaaagatt
cgtggtgctcgacgccatcgaaagatcgaataactttggaagacttgaagcggttccttg
aagttcaggagcatctgctccgacaaccatatgtcccttataacgttcacctccttcgat
ggatcgaagacaatgttggtccacagatgtatgcacaatacatggatgatgtctctggag
gtaaaacaccaatgtccccatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCACGTCTTTGATATCTATTCCCA 406 75 374 Antisense
GGACATTTGCTGCAGCTACACTTTA 530 525 569 Antisense
TGATAACTTCTACCGCTCGCGGTAT 527 561 605 Antisense
TAATTATCGGTCTTGCTGTCATCAC 250 635 636 Antisense
ATCACTGCATCACTGGATCTCTGTT 95 29 656 Antisense
TCTCTGTTCCTCTACACTGAAACGA 599 611 673 Antisense
GGTGCTCGACGCCATCGAAAGATCG 683 499 737 Antisense
GCATCTGCTCCGACAACCATATGTC 641 309 804 Antisense
CATATGTCCCTTATAACGTTCACCT 141 215 821 Antisense
GTTGGTCCACAGATGTATGCACAAT 234 437 868 Antisense
GAGGTAAAACACCAATGTCCCCATC 484 401 911 Antisense
Sequence Source Affymetrix Proprietary Database