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[172514_x_at]

Description F46F11.4 /REP_DB=WormBase Gene ID /WP=CE10602 /TR=P91302 /GB=AAK21382.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 31      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 32      6grp_total > 21      4grp_total > 32      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 534
1 2 3 4 range
A 841.1
(-3)
P 		1313.1
(3)
P 		1293.3
(2.7)
P 		1137.6
(0.8)
P 		472
B 804.3
(-3)
P 		908
(-1.5)
P 		1215.8
(3)
P 		1140.2
(1.9)
P 		412
C 947.8
(-3)
P 		1074.1
(-1.1)
P 		1318.9
(2.7)
P 		1338.5
(3)
P 		391
avr 864.4
(-3) 		1098.4
(0.4) 		1276
(3) 		1205.4
(2) 		412

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1370
1 2 3 4 5 6 range
A 1141.2
(-0.5)
P 		957.1
(-1.3)
P 		1947.3
(3)
P 		1035
(-1)
P 		946.1
(-1.4)
P 		579.4
(-3)
P 		1368
B 1255.7
(-0.9)
P 		1778.8
(2)
P 		1949.3
(3)
P 		891.2
(-3)
P 		1308.5
(-0.6)
P 		1106
(-1.8)
P 		1058
C 1122.5
(-0.3)
P 		1568.7
(3)
P 		768.3
(-3)
P 		1369.4
(1.5)
P 		988.6
(-1.3)
P 		1092.4
(-0.6)
P 		800
avr 1173.1
(-0.6) 		1434.9
(1.9) 		1555
(3) 		1098.5
(-1.4) 		1081.1
(-1.5) 		925.9
(-3) 		629
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 293
1 2 3 4 range
A 306.2
(1.9)
P 		285
(1.1)
P 		338.1
(3)
P 		166.5
(-3)
P 		172
B 256.5
(0.4)
P 		224.3
(-3)
P 		281.7
(3)
P 		243
(-1)
P 		57
C 313.7
(-0.7)
P 		308.8
(-0.9)
P 		459.7
(3)
P 		225.7
(-3)
P 		234
avr 292.1
(0.3) 		272.7
(-0.5) 		359.8
(3) 		211.7
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 451
1 2 3 4 5 6 range
A 210.4
(-1.5)
P 		340.4
(1.2)
P 		424.1
(3)
P 		262.5
(-0.4)
P 		285.5
(0.1)
P 		141.1
(-3)
P 		283
B 436.9
(0.4)
P 		592.1
(3)
P 		473.3
(1)
P 		236
(-3)
P 		531.6
(2)
P 		412.4
(-0)
P 		356
C 416.1
(1.7)
P 		478.7
(3)
P 		195.5
(-3)
P 		275.4
(-1.3)
P 		319.9
(-0.4)
P 		365.7
(0.6)
P 		283
avr 354.5
(-0.3) 		470.4
(3) 		364.3
(0) 		258
(-3) 		379
(0.4) 		306.4
(-1.6) 		212
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172514_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2232
Sequence Type Exemplar sequence
Representative Public ID F46F11.4 NCBI
Target Description F46F11.4 /REP_DB=WormBase Gene ID /WP=CE10602 /TR=P91302 /GB=AAK21382.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059239(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059239 NCBI Caenorhabditis elegans UBiquitin-Like family member (ubl-5) (ubl-5) mRNA, complete cds. 11/11 None
SNAP00000023445 ENSEMBL cdna:SNAP chromosome:CEL140:I:5607929:5608839:-1 11/11 None
GENEFINDER00000023464 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5607929:5608839:-1 11/11 None
F46F11.4 ENSEMBL cdna:known chromosome:CEL140:I:5607827:5608844:-1 gene:F46F11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5607928-5608839(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ubl-5
Ensembl F46F11.4
Entrez Gene 185872 Entrez gene
SwissProt P91302 EMBL-EBI
WormBase CE10602 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.4226.1.S1_AT similar to ubiquitin-like 5 cfa
CANINE_2:CFAAFFX.27408.1.S1_S_AT similar to ubiquitin-like 5 cfa
HG-U133_PLUS_2:218011_AT ubiquitin-like 5 hs
HG-U133A:218011_AT ubiquitin-like 5 hs
HG-FOCUS:218011_AT ubiquitin-like 5 hs
HG-U133A_2:218011_AT ubiquitin-like 5 hs
HU35KSUBA:D31544_S_AT ubiquitin-like 5 hs
U133_X3P:G13236509_3P_AT ubiquitin-like 5 hs
HG-U95C:48841_AT ubiquitin-like 5 hs
HU35KSUBD:RC_D11824_AT Ubiquitin-like 5 hs
MG-U74BV2:115511_AT similar to Chain A, Solution Structure Of The Murine Ubiquitin-Like 5 Protein From Riken Cdna 0610031k06 mm
MU19KSUBC:TC41711_AT PREDICTED: similar to Chain A, Solution Structure Of The Murine Ubiquitin-Like 5 Protein From Riken Cdna 0610031k06 [Mus musculus], mRNA sequence mm
RG-U34C:RC_AA818056_F_AT Ubiquitin-Like 5 Protein rn
RG-U34C:RC_AI101864_G_AT Ubiquitin-Like 5 Protein rn
RG-U34C:RC_AA817921_AT Ubiquitin-Like 5 Protein rn
RG-U34C:RC_AI101864_AT Ubiquitin-Like 5 Protein rn
RAE230A:1399128_AT Ubiquitin-Like 5 Protein rn
RAT230_2:1399128_AT Ubiquitin-Like 5 Protein rn
RAE230A:1371590_S_AT Ubiquitin-Like 5 Protein rn
RAT230_2:1371590_S_AT Ubiquitin-Like 5 Protein rn
RAE230A:1371589_AT Ubiquitin-Like 5 Protein rn
RAT230_2:1371589_AT Ubiquitin-Like 5 Protein rn
YEAST_2:1775449_AT Ubiquitin-like protein modifier, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Sc
Protein Similarities
Method ID Description E-Value
blast AAK21382 Ubiquitin-like family protein 5 [Caenorhabditis elegans] ref|NP_491640.1| UBiquitin-Like family member (ubl-5) [Caenorhabditis elegans] sp|P91302|UBL5_CAEEL Ubiquitin-like protein 5 emb|CAE68926.1| Hypothetical protein CBG14905 [Caenorhabditis briggsae] 3.0E-37
blast EAA05206 ENSANGP00000015674 [Anopheles gambiae str. PEST] ref|XP_309393.2| ENSANGP00000015674 [Anopheles gambiae str. PEST] 2.0E-30
blast XP_487252 PREDICTED: similar to Chain A, Solution Structure Of The Murine Ubiquitin-Like 5 Protein From Riken Cdna 0610031k06 [Mus musculus] 2.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 3.6E-12
Sequence
Target Sequence 
>C. ELEGANS:172514_X_AT
gaaatcacagtaaacgatcgactcggaaaaaaagtacgaatcaagtgcaatccatcagat
acaattggagatttgaagaagttgatcgctgcacaaactggaacacgatgggaaaagatc
gtgttgaaaaagtggtacaccatttacaaggatcatatcacattgatggattacgagatt
cacgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCACAGTAAACGATCGACTCG 499 361 19 Antisense
CACAGTAAACGATCGACTCGGAAAA 709 143 24 Antisense
GTACGAATCAAGTGCAATCCATCAG 190 459 52 Antisense
CAAGTGCAATCCATCAGATACAATT 388 183 60 Antisense
TGAAGAAGTTGATCGCTGCACAAAC 58 575 92 Antisense
TGATCGCTGCACAAACTGGAACACG 13 567 101 Antisense
GCTGCACAAACTGGAACACGATGGG 429 295 106 Antisense
AAGTGGTACACCATTTACAAGGATC 178 159 148 Antisense
ACACCATTTACAAGGATCATATCAC 388 109 155 Antisense
GGATCATATCACATTGATGGATTAC 472 511 168 Antisense
ATTGATGGATTACGAGATTCACGAG 509 3 180 Antisense
Sequence Source Affymetrix Proprietary Database