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[172511_x_at]

Description Y51H4A.24 /REP_DB=WormBase Gene ID /WP=CE25367 /TR=Q9U258 /GB=CAB63387.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 02      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 40      6grp_total > 07      4grp_total > 47      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 724
1 2 3 4 range
A 1106.9
(3)
P 		558.7
(-1.8)
P 		427.6
(-3)
P 		512
(-2.3)
P 		679
B 1140.5
(3)
P 		452.3
(-2.7)
P 		491.1
(-2.4)
P 		416.8
(-3)
P 		724
C 951.5
(3)
P 		494.5
(-3)
P 		500.2
(-2.9)
P 		583.4
(-1.8)
P 		457
avr 1066.3
(3) 		501.8
(-2.7) 		473
(-3) 		504.1
(-2.7) 		593

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 690
1 2 3 4 5 6 range
A 1060.6
(3)
P 		566.5
(-1.6)
P 		726.6
(-0.1)
P 		446.7
(-2.8)
P 		498.4
(-2.3)
P 		423
(-3)
P 		638
B 977.3
(3)
P 		651.8
(-0.9)
P 		885.9
(1.9)
P 		636.4
(-1.1)
P 		474.7
(-3)
P 		503.9
(-2.7)
P 		503
C 623.3
(2)
P 		673.6
(3)
P 		622
(2)
P 		519.6
(-0)
P 		370.6
(-3)
P 		630.8
(2.2)
P 		303
avr 887.1
(3) 		630.6
(-0.5) 		744.8
(1.1) 		534.2
(-1.8) 		447.9
(-3) 		519.2
(-2) 		439
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 328
1 2 3 4 range
A 403
(3)
P 		121.3
(-2.2)
P 		111.8
(-2.3)
P 		74.9
(-3)
P 		328
B 363.7
(3)
P 		111.7
(-2.5)
P 		113.8
(-2.5)
P 		88.8
(-3)
P 		275
C 315
(3)
P 		142.2
(-1.8)
P 		174.3
(-0.9)
P 		98.4
(-3)
P 		217
avr 360.6
(3) 		125.1
(-2.2) 		133.3
(-2) 		87.4
(-3) 		273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 195.5
(2.6)
P 		201.5
(3)
P 		158.2
(0.4)
P 		113.3
(-2.4)
P 		150.4
(-0.1)
P 		103
(-3)
P 		99
B 340
(3)
P 		217
(-1.3)
P 		215.1
(-1.4)
P 		168.5
(-3)
P 		192.9
(-2.1)
P 		187.9
(-2.3)
P 		172
C 231
(3)
P 		205.5
(1.8)
P 		158.3
(-0.4)
P 		104.5
(-3)
P 		119.9
(-2.3)
P 		211.2
(2.1)
P 		127
avr 255.5
(3) 		208
(0.8) 		177.2
(-0.7) 		128.8
(-3) 		154.4
(-1.8) 		167.4
(-1.2) 		127
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172511_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10206
Sequence Type Exemplar sequence
Representative Public ID Y51H4A.24 NCBI
Target Description Y51H4A.24 /REP_DB=WormBase Gene ID /WP=CE25367 /TR=Q9U258 /GB=CAB63387.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070580(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070580 NCBI Caenorhabditis elegans Y51H4A.24 (Y51H4A.24) mRNA, complete cds. 11/11 A 
SNAP00000000199 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16737189:16740579:-1 11/11 None
GENEFINDER00000000216 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16737189:16739250:-1 10/11 None
Y51H4A.24 ENSEMBL cdna:known chromosome:CEL140:IV:16737149:16739737:-1 gene:Y51H4A.24 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070674 6/11 Cross Hyb Matching Probes A 
SNAP00000032163 6/11 Cross Hyb Matching Probes None
GENEFINDER00000032167 6/11 Cross Hyb Matching Probes None
T28F3.8 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16737194-16739256(-) 100.0 100.0
chrIV:17305132-17306827(-) 83.26 83.26
Public Domain and Genome References
Gene Title
Ensembl Y51H4A.24
Entrez Gene 178466 Entrez gene
SwissProt Q9U258 EMBL-EBI
WormBase CE25367 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB63387 Hypothetical protein Y51H4A.24 [Caenorhabditis elegans] ref|NP_502981.1| Y51H4A.24 [Caenorhabditis elegans] 0.0
blast CAB05301 Hypothetical protein T28F3.8 [Caenorhabditis elegans] ref|NP_503075.1| T28F3.8 [Caenorhabditis elegans] 0.0
blast CAE57417 Hypothetical protein CBG00374 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:172511_X_AT
gtgccttgtaccactatggatcttatgctactttggaagatccaatgggcggaaatacac
ttgataaagttgatgagtatcagggagcagcgttgagaatgaaattgatgaatgattgct
atactagtcacgcgggctacgccgtcttcaccgattgcaccggtgctgtcagttccaaat
atgacgcttcccaaacaattccaatcgactacatttttgccgacgagggaagcttcccac
actacccaatcgtcccattcttctgtgattctaaaccaaagagcacggtggactgtgttc
ctggaactgaaagcaaatacgatattcagtttgtggccggcgagtttattgtgaatcgat
cattccagtgcagaccaggagtaggacaaattaatccaaattgtacaaatgtggattcga
atggaaattattactgtaacagagatcagctaccatacatgagaggtccaactggtcaaa
ttcccgactgtcttgggcatggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCTTGTACCACTATGGATCTTA 497 477 878 Antisense
ATTGCTATACTAGTCACGCGGGCTA 640 5 992 Antisense
CGATTGCACCGGTGCTGTCAGTTCC 41 249 1029 Antisense
TATGACGCTTCCCAAACAATTCCAA 330 657 1057 Antisense
CGACTACATTTTTGCCGACGAGGGA 476 251 1083 Antisense
GTCCCATTCTTCTGTGATTCTAAAC 459 475 1129 Antisense
AGAGCACGGTGGACTGTGTTCCTGG 83 65 1157 Antisense
ATATTCAGTTTGTGGCCGGCGAGTT 573 3 1199 Antisense
AATCGATCATTCCAGTGCAGACCAG 3 169 1231 Antisense
GAGGTCCAACTGGTCAAATTCCCGA 252 403 1340 Antisense
AATTCCCGACTGTCTTGGGCATGGC 521 175 1356 Antisense
Sequence Source Affymetrix Proprietary Database