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[172507_x_at]

Description F54A5.3B /REP_DB=WormBase Gene ID /WP=CE20862 /TR=Q9TYT2 /GB=AAC78223.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 06      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 48
(1.6)
A 		5
(-3)
A 		22.5
(-1.1)
A 		60.5
(3)
A 		56
B 27.2
(3)
A 		5.8
(-3)
A 		8.4
(-2.3)
A 		26.4
(2.8)
A 		21
C 49.9
(-0.3)
A 		10.5
(-2.9)
A 		8.9
(-3)
A 		101
(3)
A 		92
avr 41.7
(0.7) 		7.1
(-3) 		13.3
(-2.3) 		62.6
(3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 22.8
(1)
A 		5.1
(-2.6)
A 		24.5
(1.3)
A 		5
(-2.6)
A 		32.7
(3)
A 		2.9
(-3)
A 		30
B 9.4
(-2.6)
A 		9.3
(-2.7)
A 		38.7
(-0.2)
A 		5.2
(-3)
A 		6.5
(-2.9)
A 		76.9
(3)
A 		72
C 7
(-2.5)
A 		57.7
(3)
A 		50.6
(2.2)
A 		2.6
(-3)
A 		35.1
(0.5)
A 		53.5
(2.5)
A 		55
avr 13.1
(-1.7) 		24
(-0.1) 		37.9
(2) 		4.3
(-3) 		24.8
(0.1) 		44.4
(3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 17.5
(3)
A 		1.1
(-3)
A 		5.9
(-1.2)
A 		8.8
(-0.2)
A 		16
B 8.7
(3)
A 		1.4
(-3)
A 		1.9
(-2.6)
A 		5.6
(0.5)
A 		7
C 16.5
(2.8)
A 		3
(-3)
A 		3.1
(-3)
A 		17
(3)
A 		14
avr 14.2
(3) 		1.8
(-3) 		3.6
(-2.1) 		10.5
(1.2) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 4.2
(-0.7)
A 		1.8
(-2.3)
A 		5.3
(-0)
A 		1.3
(-2.6)
A 		9.9
(3)
A 		0.7
(-3)
A 		9
B 3.3
(-2.6)
A 		3.1
(-2.6)
A 		9.4
(-1.2)
A 		1.4
(-3)
A 		2.7
(-2.7)
A 		28.7
(3)
A 		27
C 2.6
(-2.3)
A 		17.6
(2.9)
A 		12.9
(1.3)
A 		0.5
(-3)
A 		11.4
(0.8)
A 		17.9
(3)
A 		17
avr 3.4
(-2.1) 		7.5
(-0.4) 		9.2
(0.3) 		1.1
(-3) 		8
(-0.2) 		15.8
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172507_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.38
Sequence Type Exemplar sequence
Representative Public ID F54A5.3B NCBI
Target Description F54A5.3B /REP_DB=WormBase Gene ID /WP=CE20862 /TR=Q9TYT2 /GB=AAC78223.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058398(10), NM_058400(11), NM_058399(11), NM_058401(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058398 NCBI Caenorhabditis elegans F54A5.3a (F54A5.3) mRNA, complete cds. 10/11 A 
NM_058400 NCBI Caenorhabditis elegans F54A5.3d (F54A5.3) mRNA, complete cds. 11/11 A 
NM_058399 NCBI Caenorhabditis elegans F54A5.3b (F54A5.3) mRNA, complete cds. 11/11 A 
NM_058401 NCBI Caenorhabditis elegans F54A5.3c (F54A5.3) mRNA, complete cds. 11/11 A 
F54A5.3a ENSEMBL cdna:known chromosome:CEL140:I:969682:973135:-1 gene:F54A5.3 10/11 A 
F54A5.3d ENSEMBL cdna:known chromosome:CEL140:I:969709:973137:-1 gene:F54A5.3 11/11 A 
F54A5.3b ENSEMBL cdna:known chromosome:CEL140:I:971901:973114:-1 gene:F54A5.3 11/11 A 
F54A5.3c ENSEMBL cdna:known chromosome:CEL140:I:971952:973139:-1 gene:F54A5.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014310 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:971969-973054(-) 100.0 100.0
Public Domain and Genome References
Ensembl F54A5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78222 Hypothetical protein F54A5.3a [Caenorhabditis elegans] ref|NP_490799.2| F54A5.3a [Caenorhabditis elegans] 1.0E-170
blast CAE61300 Hypothetical protein CBG05128 [Caenorhabditis briggsae] 1.0E-83
blast AAC78223 Hypothetical protein F54A5.3b [Caenorhabditis elegans] ref|NP_490800.1| F54A5.3b [Caenorhabditis elegans] 1.0E-37
blast AAL06026 Hypothetical protein F54A5.3d [Caenorhabditis elegans] ref|NP_490801.1| F54A5.3d [Caenorhabditis elegans] 7.0E-34
blast AAC78222 Hypothetical protein F54A5.3a [Caenorhabditis elegans] ref|NP_490799.2| F54A5.3a [Caenorhabditis elegans] 5.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 1.4E-29
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 2.8E-6
Sequence
Target Sequence 
>C. ELEGANS:172507_X_AT
tgctcaacgtggagccctcatttgcagaggagctgcggagcagcggggtttctctgtcgg
ctacttacctcggaagtgttccagtagtcgagtcaattaacgtgatggtttccgagatga
gagttcaagttgtttcagaatgtatacaacacgttgcagctactgttggcgtgactgcgg
ctcgagaaatcaatccagttgttagtaggccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCAACGTGGAGCCCTCATTTGC 627 577 14 Antisense
GAGCCCTCATTTGCAGAGGAGCTGC 192 387 25 Antisense
GAGCTGCGGAGCAGCGGGGTTTCTC 184 385 43 Antisense
GGCTACTTACCTCGGAAGTGTTCCA 114 537 72 Antisense
GAAGTGTTCCAGTAGTCGAGTCAAT 23 337 86 Antisense
GAGTCAATTAACGTGATGGTTTCCG 486 399 103 Antisense
GATGGTTTCCGAGATGAGAGTTCAA 321 407 117 Antisense
AACACGTTGCAGCTACTGTTGGCGT 70 127 161 Antisense
GCAGCTACTGTTGGCGTGACTGCGG 513 313 169 Antisense
GACTGCGGCTCGAGAAATCAATCCA 555 375 186 Antisense
TCAATCCAGTTGTTAGTAGGCCGAC 502 631 203 Antisense
Sequence Source Affymetrix Proprietary Database