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[172494_at]

Description H12I13.3 /REP_DB=WormBase Gene ID /WP=CE23805 /TR=Q9N5M7 /GB=AAF39882.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 08      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 0.4
(-3)
A 		0.9
(-2.8)
A 		2
(-2.4)
A 		17.8
(3)
A 		17
B 0.8
(-3)
A 		9.8
(-0.5)
A 		18
(1.7)
A 		22.7
(3)
A 		22
C 0.7
(-3)
A 		1.4
(-2.9)
A 		0.4
(-3)
A 		49.9
(3)
A 		50
avr 0.6
(-3) 		4
(-2.3) 		6.8
(-1.7) 		30.1
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 0.7
(-1.9)
A 		2.1
(3)
A 		0.6
(-2.3)
A 		1.9
(2.3)
A 		1
(-0.9)
A 		0.4
(-3)
A 		2
B 1.2
(3)
A 		0.7
(-0.3)
A 		0.4
(-2.3)
A 		0.8
(0.3)
A 		0.3
(-3)
A 		1.2
(3)
A 		1
C 0.2
(-3)
A 		1.2
(-2.6)
A 		1.4
(-2.6)
A 		17.1
(3)
A 		0.7
(-2.8)
A 		0.4
(-2.9)
A 		17
avr 0.7
(-3) 		1.3
(-2.4) 		0.8
(-2.9) 		6.6
(3) 		0.7
(-3) 		0.7
(-3) 		6
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 0.2
(-3)
A 		0.2
(-3)
A 		0.5
(-2.3)
A 		2.6
(3)
A 		2
B 0.2
(-3)
A 		2.4
(-0.1)
A 		4.2
(2.2)
A 		4.8
(3)
A 		5
C 0.2
(-2.9)
A 		0.4
(-2.8)
A 		0.1
(-3)
A 		8.4
(3)
A 		8
avr 0.2
(-3) 		1
(-2.1) 		1.6
(-1.4) 		5.3
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 0.1
(-3)
A 		0.7
(3)
A 		0.1
(-3)
A 		0.5
(1)
A 		0.3
(-1)
A 		0.1
(-3)
A 		1
B 0.4
(3)
A 		0.2
(-1)
A 		0.1
(-3)
A 		0.2
(-1)
A 		0.1
(-3)
A 		0.4
(3)
A 		0
C 0.1
(-3)
A 		0.4
(-2.5)
A 		0.3
(-2.6)
A 		3.4
(3)
A 		0.2
(-2.8)
A 		0.1
(-3)
A 		3
avr 0.2
(-3) 		0.4
(-2) 		0.2
(-3) 		1.4
(3) 		0.2
(-3) 		0.2
(-3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172494_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4345
Sequence Type Exemplar sequence
Representative Public ID H12I13.3 NCBI
Target Description H12I13.3 /REP_DB=WormBase Gene ID /WP=CE23805 /TR=Q9N5M7 /GB=AAF39882.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062813(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062813 NCBI Caenorhabditis elegans H12I13.3 (H12I13.3) mRNA, complete cds. 11/11 None
GENEFINDER00000034378 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6065959:6073205:-1 11/11 None
H12I13.3 ENSEMBL cdna:known chromosome:CEL140:II:6071340:6073205:-1 gene:H12I13.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034374 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6071336-6073202(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H12I13.3
Entrez Gene 186734 Entrez gene
SwissProt Q9N5M7 EMBL-EBI
WormBase CE23805 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39882 Hypothetical protein H12I13.3 [Caenorhabditis elegans] ref|NP_495214.1| H12I13.3 [Caenorhabditis elegans] 0.0
blast AAF39880 Hypothetical protein H12I13.2 [Caenorhabditis elegans] ref|NP_495213.1| H12I13.2 [Caenorhabditis elegans] 1.0E-172
blast AAK84500 Hypothetical protein C33E10.6 [Caenorhabditis elegans] ref|NP_510803.1| C33E10.6 [Caenorhabditis elegans] 1.0E-93
blast AAK84500 Hypothetical protein C33E10.6 [Caenorhabditis elegans] ref|NP_510803.1| C33E10.6 [Caenorhabditis elegans] 4.0E-87
blast CAE69070 Hypothetical protein CBG15088 [Caenorhabditis briggsae] 7.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.6E-11
Sequence
Target Sequence 
>C. ELEGANS:172494_AT
gagtcaatattagattagtcaccgatgatgatagtgctttgaagttaattagaaaaccaa
atttcaaaaatagtactatcataactgaagatttgttaagtgtagaattaacgaaaacta
agttggtatttgataagccaatatattttcactacgatgtaatgcaaaaacattatggag
ataaaatcaaattatgttatcaggatactgacagtttgatatatgaggtagaaaccgagg
atatttatgaagatatgggtaatatgaaagagtggtttgactttagtgactatcctaaga
atcatccgttatatgatgaaagtaataagaaagttatcggaaaattcaaggatgagttga
acggtaaaaatatggatgaaggagtgttcttaaagcctaaacagtacgattttaatgtag
aaggtgcagagaaaaagaaatccaaaggtgtgaaaaagaacgttactaaaacgttgactt
tagatgacttcaagcgatgtctattccaaaatgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCAATATTAGATTAGTCACCGA 688 399 1085 Antisense
GATTAGTCACCGATGATGATAGTGC 165 431 1097 Antisense
ATATTTTCACTACGATGTAATGCAA 114 19 1227 Antisense
GATACTGACAGTTTGATATATGAGG 589 423 1288 Antisense
GAGGTAGAAACCGAGGATATTTATG 483 401 1309 Antisense
GTGGTTTGACTTTAGTGACTATCCT 401 489 1356 Antisense
GACTATCCTAAGAATCATCCGTTAT 491 371 1372 Antisense
GGAGTGTTCTTAAAGCCTAAACAGT 234 511 1465 Antisense
AAAACGTTGACTTTAGATGACTTCA 476 131 1552 Antisense
GATGACTTCAAGCGATGTCTATTCC 461 409 1567 Antisense
CAAGCGATGTCTATTCCAAAATGAA 184 187 1575 Antisense
Sequence Source Affymetrix Proprietary Database