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[172484_x_at]

Description F22D3.5 /REP_DB=WormBase Gene ID /WP=CE02674 /TR=Q19717 /GB=AAK31495.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 01      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 2.7
(-3)
A 		12
(-2.4)
A 		37.7
(-0.7)
A 		93
(3)
A 		90
B 36.6
(0.3)
A 		3.1
(-3)
A 		31.5
(-0.2)
A 		63.6
(3)
A 		61
C 16.5
(-2.6)
A 		10.3
(-3)
A 		21
(-2.3)
A 		108.7
(3)
P 		98
avr 18.6
(-2.2) 		8.5
(-3) 		30.1
(-1.4) 		88.4
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 48.1
(0)
A 		30.3
(-2.1)
A 		22.6
(-3)
A 		51
(0.3)
A 		44.3
(-0.4)
A 		73.6
(3)
A 		51
B 13.9
(-2.2)
A 		4.1
(-3)
A 		40.8
(0.2)
P 		28.2
(-0.9)
A 		52.3
(1.2)
P 		73.3
(3)
P 		69
C 22
(-2.4)
A 		39.9
(-0.9)
A 		14
(-3)
A 		37.8
(-1.1)
A 		39.3
(-1)
M 		89.7
(3)
P 		76
avr 28
(-2.6) 		24.8
(-3) 		25.8
(-2.9) 		39
(-1.4) 		45.3
(-0.7) 		78.9
(3) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 1
(-3)
A 		2.6
(-2.2)
A 		9.9
(1.2)
A 		13.6
(3)
A 		13
B 11.7
(2.1)
A 		0.8
(-3)
A 		7.3
(0.1)
A 		13.5
(3)
A 		13
C 5.5
(-2)
A 		3
(-3)
A 		7.3
(-1.3)
A 		18.3
(3)
P 		15
avr 6.1
(-1.2) 		2.1
(-3) 		8.2
(-0.2) 		15.1
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 8.9
(-1.2)
A 		10.8
(-0.3)
A 		4.9
(-3)
A 		12.9
(0.7)
A 		13.4
(0.9)
A 		17.9
(3)
A 		13
B 4.8
(-2.2)
A 		1.4
(-3)
A 		9.9
(-1)
P 		7.5
(-1.6)
A 		21.2
(1.6)
P 		27.3
(3)
P 		26
C 8.2
(-2)
A 		12.2
(-1)
A 		3.6
(-3)
A 		7.6
(-2.1)
A 		12.7
(-0.9)
M 		30
(3)
P 		26
avr 7.3
(-2.6) 		8.1
(-2.4) 		6.1
(-3) 		9.3
(-2) 		15.8
(0.1) 		25.1
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172484_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5269
Sequence Type Exemplar sequence
Representative Public ID F22D3.5 NCBI
Target Description F22D3.5 /REP_DB=WormBase Gene ID /WP=CE02674 /TR=Q19717 /GB=AAK31495.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063022(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063022 NCBI Caenorhabditis elegans F22D3.5 (F22D3.5) mRNA, complete cds. 11/11 None
F22D3.5 ENSEMBL cdna:known chromosome:CEL140:II:6938514:6938942:-1 gene:F22D3.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075836 3/11 Cross Hyb Matching Probes A 
SNAP00000029215 6/11 Cross Hyb Matching Probes None
GENEFINDER00000029228 3/11 Cross Hyb Matching Probes None
C52B11.4 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1285059-1285489(+) 99.21 99.21
chrI:5142713-5143143(+) 98.81 98.81
chrIV:11468234-11468664(+) 99.21 99.21
chrII:6938509-6938938(-) 100.0 100.0
chrII:14000638-14001068(-) 98.41 98.41
chrIII:121733-122163(-) 96.03 96.03
Public Domain and Genome References
Gene Title
Ensembl F22D3.5
Entrez Gene 184826 Entrez gene
SwissProt Q19717 EMBL-EBI
WormBase CE02674 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31495 Hypothetical protein F22D3.5 [Caenorhabditis elegans] ref|NP_495423.1| F22D3.5 [Caenorhabditis elegans] 5.0E-29
Sequence
Target Sequence 
>C. ELEGANS:172484_X_AT
cgtcttttttcttgcgaaaagcactttaatctaaatatgtcaaccccagcccgtaaaccc
agaaaggaaaccgaaaactgtaagtttgtcagctcgaagtctttgagtgctgattttgga
gacgttttctcccgacagtttgaacgcaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCTTTTTTCTTGCGAAAAGCACT 353 245 79 Antisense
TCTTGCGAAAAGCACTTTAATCTAA 59 617 88 Antisense
AATCTAAATATGTCAACCCCAGCCC 516 169 106 Antisense
AAACTGTAAGTTTGTCAGCTCGAAG 159 127 153 Antisense
AAGTTTGTCAGCTCGAAGTCTTTGA 682 153 160 Antisense
TGTCAGCTCGAAGTCTTTGAGTGCT 86 557 165 Antisense
GAAGTCTTTGAGTGCTGATTTTGGA 229 337 174 Antisense
GAGTGCTGATTTTGGAGACGTTTTC 351 401 183 Antisense
TTGGAGACGTTTTCTCCCGACAGTT 260 701 194 Antisense
TTTTCTCCCGACAGTTTGAACGCAA 584 675 203 Antisense
TCTCCCGACAGTTTGAACGCAAAAA 451 609 206 Antisense
Sequence Source Affymetrix Proprietary Database