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[172482_x_at]

Description Y53F4A.2 /REP_DB=WormBase Gene ID /WP=CE22382 /TR=Q9NAC9 /GB=CAB54462.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 20      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 59.2
(-1.4)
A 		30
(-3)
A 		57.6
(-1.5)
A 		137.1
(3)
A 		107
B 39.4
(-3)
A 		94.1
(3)
A 		50.2
(-1.8)
A 		44.9
(-2.4)
A 		55
C 88.6
(1.8)
A 		67.5
(-0.1)
A 		34.8
(-3)
A 		102
(3)
A 		67
avr 62.4
(-1.1) 		63.9
(-0.9) 		47.5
(-3) 		94.7
(3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 208
1 2 3 4 5 6 range
A 45.3
(-2.4)
A 		33.2
(-2.9)
A 		51.8
(-2.1)
M 		30
(-3)
A 		171.6
(3)
P 		156
(2.3)
P 		142
B 59.8
(-2.2)
A 		65.2
(-2.1)
A 		42.3
(-2.7)
A 		33.3
(-3)
A 		238.1
(3)
P 		221.2
(2.5)
P 		205
C 54.2
(-2.3)
A 		39.5
(-2.7)
A 		64.8
(-2)
A 		30.6
(-3)
A 		231.3
(3)
P 		198.8
(2)
P 		201
avr 53.1
(-2.3) 		46
(-2.5) 		53
(-2.3) 		31.3
(-3) 		213.7
(3) 		192
(2.3) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 21.5
(3)
A 		6.5
(-3)
A 		15
(0.4)
A 		20.1
(2.4)
A 		15
B 12.6
(-1.7)
A 		23.2
(3)
A 		11.6
(-2.1)
A 		9.6
(-3)
A 		14
C 29.3
(3)
A 		19.4
(-0.5)
A 		12.1
(-3)
A 		17.2
(-1.2)
A 		17
avr 21.1
(3) 		16.4
(-0.4) 		12.9
(-3) 		15.6
(-1) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 8.3
(-2.9)
A 		11.8
(-2.4)
A 		11.3
(-2.5)
M 		7.6
(-3)
A 		51.8
(3)
P 		38
(1.1)
P 		44
B 20.8
(-2.2)
A 		21.7
(-2.1)
A 		10.3
(-2.9)
A 		8.8
(-3)
A 		96.7
(3)
P 		82.5
(2)
P 		88
C 20.1
(-1.8)
A 		12
(-2.5)
A 		16.5
(-2.1)
A 		6.2
(-3)
A 		74.8
(3)
P 		66.6
(2.3)
P 		69
avr 16.4
(-2.2) 		15.2
(-2.3) 		12.7
(-2.5) 		7.5
(-3) 		74.4
(3) 		62.4
(1.9) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172482_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3107
Sequence Type Exemplar sequence
Representative Public ID Y53F4A.2 NCBI
Target Description Y53F4A.2 /REP_DB=WormBase Gene ID /WP=CE22382 /TR=Q9NAC9 /GB=CAB54462.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064679(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064679 NCBI Caenorhabditis elegans Y53F4A.2 (Y53F4A.2) mRNA, complete cds. 11/11 None
GENEFINDER00000008826 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14876037:14876934:-1 11/11 None
Y53F4A.2 ENSEMBL cdna:known chromosome:CEL140:II:14876037:14876934:-1 gene:Y53F4A.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070007 1/11 Cross Hyb Matching Probes A 
NM_064299 1/11 Cross Hyb Matching Probes None
SNAP00000017445 1/11 Cross Hyb Matching Probes None
SNAP00000042645 1/11 Cross Hyb Matching Probes None
GENEFINDER00000042652 1/11 Cross Hyb Matching Probes None
Y38E10A.17 1/11 Cross Hyb Matching Probes A 
K08E7.4 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14876033-14876931(-) 85.61 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4A.2
Entrez Gene 190205 Entrez gene
SwissProt Q9NAC9 EMBL-EBI
WormBase CE22382 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54462 Hypothetical protein Y53F4A.2 [Caenorhabditis elegans] ref|NP_497080.1| Y53F4A.2 [Caenorhabditis elegans] 3.0E-19
blast CAB01227 Hypothetical protein K08E7.4 [Caenorhabditis elegans] ref|NP_502408.1| K08E7.4 [Caenorhabditis elegans] 6.0E-16
Sequence
Target Sequence 
>C. ELEGANS:172482_X_AT
ctattaactctcaatgcactgccatttgtgctggtcaattgtgggggtaagaagaagcca
gcgcctccgccaaaagccgccgctaagcctatggcagcgcccgctggaggagctggtgga
gctggtggagctggtggagccggaaaaagcgactcattcgattcgaagaaacaggacatt
ccatcgtcaaaaaaggaagagccagtggctccaaagaaagagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTATTAACTCTCAATGCACTGCCAT 122 225 16 Antisense
AACTCTCAATGCACTGCCATTTGTG 630 139 21 Antisense
ACTGCCATTTGTGCTGGTCAATTGT 405 99 33 Antisense
GCTGGTCAATTGTGGGGGTAAGAAG 216 291 45 Antisense
GGGGTAAGAAGAAGCCAGCGCCTCC 214 497 59 Antisense
CCCGCTGGAGGAGCTGGTGGAGCTG 523 259 115 Antisense
GTGGAGCTGGTGGAGCTGGTGGAGC 514 489 131 Antisense
GCTGGTGGAGCCGGAAAAAGCGACT 297 293 145 Antisense
AAAAGCGACTCATTCGATTCGAAGA 710 131 160 Antisense
GAAACAGGACATTCCATCGTCAAAA 31 357 183 Antisense
GAGCCAGTGGCTCCAAAGAAAGAGG 490 385 214 Antisense
Sequence Source Affymetrix Proprietary Database