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[172478_x_at]

Description ZK250.8 /REP_DB=WormBase Gene ID /WP=CE15281 /TR=O17304 /GB=AAB71077.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 71      4grp_total > 08      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 66.2
(-3)
A 		82.6
(-2.2)
A 		138.7
(0.5)
P 		192.1
(3)
P 		126
B 41.7
(-2.7)
A 		68.9
(-1)
A 		37.1
(-3)
A 		130.6
(3)
M 		94
C 69.6
(-2.4)
A 		59.1
(-3)
A 		76.1
(-2.1)
P 		169
(3)
P 		110
avr 59.2
(-3) 		70.2
(-2.4) 		84
(-1.6) 		163.9
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 28
(-2.2)
A 		45.4
(-1.5)
A 		159.8
(3)
P 		128.5
(1.8)
M 		71.9
(-0.5)
A 		7.6
(-3)
A 		152
B 55.3
(-3)
A 		62.1
(-2.1)
A 		100.6
(3)
P 		99.4
(2.8)
P 		56
(-2.9)
A 		63.6
(-1.9)
A 		45
C 28.3
(-2.1)
A 		95.1
(1.5)
P 		10.6
(-3)
A 		123.4
(3)
A 		49.3
(-0.9)
A 		39.4
(-1.5)
A 		113
avr 37.2
(-3) 		67.5
(-0.7) 		90.3
(1) 		117.1
(3) 		59.1
(-1.3) 		36.9
(-3) 		80
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 24.1
(-1)
A 		17.9
(-3)
A 		36.3
(3)
P 		28.1
(0.3)
P 		18
B 13.3
(-1.5)
A 		17
(-0.4)
A 		8.6
(-3)
A 		27.8
(3)
M 		19
C 23
(0.1)
A 		17
(-3)
A 		26.5
(2)
P 		28.5
(3)
P 		12
avr 20.1
(-1.4) 		17.3
(-3) 		23.8
(0.6) 		28.1
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 5.2
(-2.4)
A 		16.1
(-0.4)
A 		34.8
(3)
P 		32.6
(2.6)
M 		21.7
(0.6)
A 		1.9
(-3)
A 		33
B 19.3
(-3)
A 		20.7
(-1.8)
A 		24.4
(1.4)
P 		26.3
(3)
P 		22.8
(0)
A 		23.7
(0.8)
A 		7
C 10.5
(-1.2)
A 		29
(3)
P 		2.7
(-3)
A 		24.8
(2)
A 		16
(0)
A 		13.2
(-0.6)
A 		26
avr 11.7
(-3) 		21.9
(0.8) 		20.6
(0.3) 		27.9
(3) 		20.2
(0.1) 		12.9
(-2.6) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172478_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5326
Sequence Type Exemplar sequence
Representative Public ID ZK250.8 NCBI
Target Description ZK250.8 /REP_DB=WormBase Gene ID /WP=CE15281 /TR=O17304 /GB=AAB71077.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061738(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061738 NCBI Caenorhabditis elegans ZK250.8 (ZK250.8) mRNA, complete cds. 11/11 None
SNAP00000013031 ENSEMBL cdna:SNAP chromosome:CEL140:II:1956051:1957583:-1 9/11 None
GENEFINDER00000013042 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1956051:1958754:-1 11/11 None
ZK250.8 ENSEMBL cdna:known chromosome:CEL140:II:1956051:1958256:-1 gene:ZK250.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_061749 2/11 Cross Hyb Matching Probes A 
NM_061745 4/11 Cross Hyb Matching Probes A 
NM_061737 1/11 Cross Hyb Matching Probes A 
SNAP00000002419 3/11 Cross Hyb Matching Probes None
GENEFINDER00000002444 3/11 Cross Hyb Matching Probes None
SNAP00000013030 1/11 Cross Hyb Matching Probes None
GENEFINDER00000013039 1/11 Cross Hyb Matching Probes None
SNAP00000024877 3/11 Cross Hyb Matching Probes None
GENEFINDER00000024883 4/11 Cross Hyb Matching Probes None
ZK250.7 1/11 Cross Hyb Matching Probes A 
C40D2.2.2 4/11 Cross Hyb Matching Probes None
C40D2.2.1 4/11 Cross Hyb Matching Probes None
F59H6.4 2/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1956049-1958255(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK250.8
Entrez Gene 173561 Entrez gene
SwissProt O17304 EMBL-EBI
WormBase CE15281 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71077 Hypothetical protein ZK250.8 [Caenorhabditis elegans] ref|NP_494139.1| ZK250.8 [Caenorhabditis elegans] 0.0
blast AAC26265 Hypothetical protein C40D2.2 [Caenorhabditis elegans] ref|NP_494146.1| C40D2.2 [Caenorhabditis elegans] 0.0
blast AAB70958 Hypothetical protein C46F9.3 [Caenorhabditis elegans] ref|NP_494116.1| C46F9.3 [Caenorhabditis elegans] 0.0
blast AAB70956 Hypothetical protein C46F9.2 [Caenorhabditis elegans] ref|NP_494117.1| C46F9.2 [Caenorhabditis elegans] 1.0E-180
blast AAB71077 Hypothetical protein ZK250.8 [Caenorhabditis elegans] ref|NP_494139.1| ZK250.8 [Caenorhabditis elegans] 1.0E-173
Protein Domains
Database ID Description E-Value
Pfam IPR002083 EMBL-EBI MATH 1.0E-16
Pfam IPR002083 EMBL-EBI MATH 6.1E-35
Pfam IPR002083 EMBL-EBI MATH 1.9E-5
Pfam IPR002083 EMBL-EBI MATH 2.3E-13
Pfam IPR002083 EMBL-EBI MATH 8.2E-45
Pfam IPR002083 EMBL-EBI MATH 6.1E-35
Pfam IPR002083 EMBL-EBI MATH 3.9E-49
Pfam IPR002083 EMBL-EBI MATH 1.1E-39
Pfam IPR002083 EMBL-EBI MATH 6.1E-35
Pfam IPR002083 EMBL-EBI MATH 3.9E-49
Sequence
Target Sequence 
>C. ELEGANS:172478_X_AT
aaggctatctttggagctacgtcgaattggggattccagtttattagttggaatgatatg
gaaaacgattatgtgaccaatggcagtatcgatgtggaagttcatgtcaaaatagttcaa
atcacagaattgtcatgccaagagaaatcatttgtactatcccatactgtcaagaacatg
tcaagtattgaagaaggaaggagcatctttacggacattcaaactcggtttaacattcca
tggaaattgaaaattcgaaagcaagacgggttttttgaattatttctcctatgtgacaaa
caactatgtaaatccagaaaatggtcaattgaaactgaatttcaatttaaaatagtgtca
tccaatgggagaagtttattgttaaatgtaaaaccatatgtgtttgataaagcaaccggt
tacggatggcgtttcatgagatggaacagtttggaggaaaaatacatagtcgtcgatact
ctcacaattgaagctcacgtgaagattcttaatgtgattggaattgaagatgaaaccact
gttgagaaaccttctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGCTATCTTTGGAGCTACGTCGA 424 159 712 Antisense
GAATTGGGGATTCCAGTTTATTAGT 37 187 735 Antisense
TGTGACCAATGGCAGTATCGATGTG 515 555 783 Antisense
TCATTTGTACTATCCCATACTGTCA 639 619 859 Antisense
GGAGCATCTTTACGGACATTCAAAC 269 521 911 Antisense
CAAACTCGGTTTAACATTCCATGGA 372 191 931 Antisense
GAATTATTTCTCCTATGTGACAAAC 108 325 988 Antisense
CCGGTTACGGATGGCGTTTCATGAG 599 265 1127 Antisense
AATACATAGTCGTCGATACTCTCAC 682 171 1172 Antisense
GATACTCTCACAATTGAAGCTCACG 118 425 1186 Antisense
GAAACCACTGTTGAGAAACCTTCTG 561 357 1243 Antisense
Sequence Source Affymetrix Proprietary Database