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[172474_x_at]

Description F35C5.10 /REP_DB=WormBase Gene ID /WP=CE15951 /TR=O45446 /GB=CAB03060.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 91      6grp_total > 93      4grp_total > 50      6grp_cgc > 98      6grp_high1_sub > 05      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 526
1 2 3 4 range
A 42.3
(-2.7)
A 		538.6
(3)
P 		379.9
(1.2)
P 		12.4
(-3)
A 		526
B 17.1
(-3)
A 		479.2
(3)
P 		446.9
(2.6)
P 		19
(-3)
A 		462
C 13.1
(-3)
A 		532.8
(3)
P 		361
(1)
P 		60
(-2.5)
A 		520
avr 24.2
(-3) 		516.9
(3) 		395.9
(1.5) 		30.5
(-2.9) 		493

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 575
1 2 3 4 5 6 range
A 172
(-2.8)
P 		248.4
(-1.9)
P 		149.6
(-3)
P 		386.2
(-0.4)
P 		697.4
(3)
P 		230.1
(-2.1)
P 		548
B 122.2
(-3)
A 		244
(-1.7)
P 		182
(-2.3)
P 		309.8
(-0.9)
P 		670.8
(3)
P 		209.7
(-2)
P 		549
C 158.7
(-3)
P 		289.2
(-1.2)
P 		219.5
(-2.2)
P 		385.5
(0.1)
P 		601.2
(3)
P 		196.3
(-2.5)
P 		443
avr 151
(-3) 		260.5
(-1.7) 		183.7
(-2.6) 		360.5
(-0.5) 		656.5
(3) 		212
(-2.3) 		506
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 15.4
(-2.3)
A 		116.9
(3)
P 		99.3
(2.1)
P 		1.8
(-3)
A 		115
B 5.5
(-2.9)
A 		118.4
(3)
P 		103.6
(2.2)
P 		4
(-3)
A 		114
C 4.3
(-3)
A 		153.2
(3)
P 		125.8
(1.9)
P 		10.1
(-2.8)
A 		149
avr 8.4
(-2.9) 		129.5
(3) 		109.6
(2) 		5.3
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 31.7
(-3)
P 		88.3
(-1.1)
P 		32.6
(-3)
P 		98
(-0.8)
P 		210.5
(3)
P 		56
(-2.2)
P 		179
B 42.5
(-3)
A 		81.2
(-2)
P 		44.2
(-3)
P 		82.1
(-2)
P 		272.5
(3)
P 		78.2
(-2.1)
P 		230
C 58.8
(-2.9)
P 		88.2
(-1.6)
P 		55.9
(-3)
P 		77.5
(-2.1)
P 		194.5
(3)
P 		65.7
(-2.6)
P 		139
avr 44.3
(-3) 		85.9
(-1.6) 		44.2
(-3) 		85.9
(-1.6) 		225.8
(3) 		66.6
(-2.3) 		182
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172474_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3661
Sequence Type Exemplar sequence
Representative Public ID F35C5.10 NCBI
Target Description F35C5.10 /REP_DB=WormBase Gene ID /WP=CE15951 /TR=O45446 /GB=CAB03060.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064346(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064346 NCBI Caenorhabditis elegans Nematode Specific Peptide family, group B family member (nspb-11) (nspb-11) mRNA, complete cds. 11/11 None
SNAP00000007551 ENSEMBL cdna:SNAP chromosome:CEL140:II:12907302:12907596:1 11/11 None
GENEFINDER00000007555 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12904316:12907596:1 11/11 None
F35C5.10 ENSEMBL cdna:known chromosome:CEL140:II:12907302:12907596:1 gene:F35C5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12907298-12907593(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nspb-11
Ensembl F35C5.10
Entrez Gene 185265 Entrez gene
SwissProt O45446 EMBL-EBI
WormBase CE15951 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03059 Hypothetical protein F35C5.9 [Caenorhabditis elegans] ref|NP_496746.1| F35C5.9 [Caenorhabditis elegans] 0.0
blast CAE60630 Hypothetical protein CBG04273 [Caenorhabditis briggsae] 1.0E-138
blast CAB03057 Hypothetical protein F35C5.8 [Caenorhabditis elegans] ref|NP_496745.1| F35C5.8 [Caenorhabditis elegans] 1.0E-88
blast CAB03060 Hypothetical protein F35C5.10 [Caenorhabditis elegans] ref|NP_496747.1| Nematode Specific Peptide family, group B family member (nspb-11) [Caenorhabditis elegans] 7.0E-20
blast NP_498194 Nematode Specific Peptide family, group B family member (nspb-12) [Caenorhabditis elegans] gb|AAA50700.2| Hypothetical protein F09F7.8 [Caenorhabditis elegans] 4.0E-13
blast CAE64304 Hypothetical protein CBG08980 [Caenorhabditis briggsae] 1.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 6.5E-29
Sequence
Target Sequence 
>C. ELEGANS:172474_X_AT
tcctctgcgccatgatcattggctcccagcaacaagttgttgtcccagcgtactacccat
catactatccatcagtatattctccatatgttgctgctgccgcctatccatcagtctggg
catggggatccaacaagaacaaggacgactcggaagctccacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCTGCGCCATGATCATTGGCTC 437 591 41 Antisense
TCTGCGCCATGATCATTGGCTCCCA 382 609 44 Antisense
TGATCATTGGCTCCCAGCAACAAGT 337 565 53 Antisense
CATTGGCTCCCAGCAACAAGTTGTT 665 217 57 Antisense
GCAACAAGTTGTTGTCCCAGCGTAC 309 317 69 Antisense
ACTACCCATCATACTATCCATCAGT 705 101 92 Antisense
ACTATCCATCAGTATATTCTCCATA 81 103 104 Antisense
TATATTCTCCATATGTTGCTGCTGC 69 663 116 Antisense
CGCCTATCCATCAGTCTGGGCATGG 377 257 141 Antisense
GTCTGGGCATGGGGATCCAACAAGA 306 471 154 Antisense
ACAAGGACGACTCGGAAGCTCCACG 373 111 179 Antisense
Sequence Source Affymetrix Proprietary Database