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[172469_x_at]

Description C01G12.6 /REP_DB=WormBase Gene ID /WP=CE15575 /TR=O45237 /GB=CAB03816.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_high_8_8 > 55      6grp_highest_nonorm > 92      6grp_total > 94      4grp_total > 48      6grp_cgc > 89      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 869
1 2 3 4 range
A 32.2
(-3)
A 		475.8
(0.8)
P 		724.8
(3)
P 		83.9
(-2.6)
A 		693
B 67.6
(-3)
P 		434.9
(-0.4)
P 		901.4
(3)
P 		88.7
(-2.8)
A 		834
C 57.8
(-3)
P 		469.9
(1)
P 		679
(3)
P 		103.4
(-2.6)
P 		621
avr 52.5
(-3) 		460.2
(0.4) 		768.4
(3) 		92
(-2.7) 		716

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 370
1 2 3 4 5 6 range
A 228.9
(-1.8)
P 		268.2
(-1)
P 		170.7
(-3)
P 		236.4
(-1.6)
P 		456.3
(3)
P 		286.6
(-0.6)
P 		286
B 135.2
(-3)
P 		289.2
(-0.5)
P 		258.8
(-1)
P 		220.8
(-1.6)
P 		505.4
(3)
P 		329.9
(0.2)
P 		370
C 156.8
(-3)
P 		287.7
(-0.3)
P 		193.5
(-2.2)
P 		288.2
(-0.3)
P 		446.7
(3)
P 		313.1
(0.2)
P 		290
avr 173.6
(-3) 		281.7
(-0.8) 		207.7
(-2.3) 		248.5
(-1.5) 		469.5
(3) 		309.9
(-0.2) 		296
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 225
1 2 3 4 range
A 11.7
(-3)
A 		103.3
(0.1)
P 		189.5
(3)
P 		12.3
(-3)
A 		178
B 21.6
(-2.9)
P 		107.4
(-0.2)
P 		208.9
(3)
P 		18.9
(-3)
A 		190
C 19.1
(-3)
P 		135.1
(0.2)
P 		236.6
(3)
P 		17.4
(-3)
P 		219
avr 17.5
(-3) 		115.3
(0) 		211.7
(3) 		16.2
(-3) 		196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 42.2
(-2.7)
P 		95.4
(0.5)
P 		37.2
(-3)
P 		59.9
(-1.6)
P 		137.7
(3)
P 		69.8
(-1.1)
P 		101
B 47
(-3)
P 		96.3
(-1.1)
P 		62.8
(-2.4)
P 		58.5
(-2.6)
P 		205.3
(3)
P 		123
(-0.1)
P 		158
C 58.1
(-2.4)
P 		87.8
(-0.6)
P 		49.2
(-3)
P 		58
(-2.4)
P 		144.6
(3)
P 		104.8
(0.5)
P 		95
avr 49.1
(-3) 		93.2
(-0.7) 		49.7
(-3) 		58.8
(-2.5) 		162.5
(3) 		99.2
(-0.3) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172469_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3221
Sequence Type Exemplar sequence
Representative Public ID C01G12.6 NCBI
Target Description C01G12.6 /REP_DB=WormBase Gene ID /WP=CE15575 /TR=O45237 /GB=CAB03816.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064631(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064631 NCBI Caenorhabditis elegans Nematode Specific Peptide family, group B family member (nspb-10) (nspb-10) mRNA, complete cds. 11/11 None
SNAP00000024270 ENSEMBL cdna:SNAP chromosome:CEL140:II:14587848:14588132:-1 11/11 None
GENEFINDER00000024282 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14587848:14588463:-1 11/11 None
C01G12.6 ENSEMBL cdna:known chromosome:CEL140:II:14587848:14588132:-1 gene:C01G12.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064628 7/11 Cross Hyb Matching Probes A 
NM_001026732 2/11 Cross Hyb Matching Probes None
NM_001026733 3/11 Cross Hyb Matching Probes None
SNAP00000024263 7/11 Cross Hyb Matching Probes None
SNAP00000024264 2/11 Cross Hyb Matching Probes None
SNAP00000024265 3/11 Cross Hyb Matching Probes None
GENEFINDER00000024278 7/11 Cross Hyb Matching Probes None
GENEFINDER00000024283 3/11 Cross Hyb Matching Probes None
GENEFINDER00000024286 2/11 Cross Hyb Matching Probes None
C01G12.2 7/11 Cross Hyb Matching Probes A 
C01G12.10 2/11 Cross Hyb Matching Probes None
C01G12.11 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14578200-14578485(+) 98.33 98.33
chrII:14587844-14588129(-) 100.0 100.0
chrII:14580224-14580503(-) 92.08 92.08
Public Domain and Genome References
Gene Title
Gene Symbol nspb-10
Ensembl C01G12.6
Entrez Gene 182088 Entrez gene
SwissProt O45237 EMBL-EBI
WormBase CE15575 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03816 Hypothetical protein C01G12.6 [Caenorhabditis elegans] ref|NP_497032.1| Nematode Specific Peptide family, group B family member (nspb-10) [Caenorhabditis elegans] 4.0E-19
blast CAB03813 Hypothetical protein C01G12.2 [Caenorhabditis elegans] ref|NP_497029.2| Nematode Specific Peptide family, group B family member (nspb-7) [Caenorhabditis elegans] 1.0E-18
blast T18827 hypothetical protein C01G12.2 - Caenorhabditis elegans 1.0E-18
blast CAB03816 Hypothetical protein C01G12.6 [Caenorhabditis elegans] ref|NP_497032.1| Nematode Specific Peptide family, group B family member (nspb-10) [Caenorhabditis elegans] 3.0E-16
Sequence
Target Sequence 
>C. ELEGANS:172469_X_AT
tctgcgccacgatcatgggatctcatcaacaagtggttgtaccagcctactctgcagcct
actacccatcagcatatgcttatccatcagtatattctccttatgtatctgcctatccat
cagtctgggcttggggatccaacaagaacaaggatgatgctccacgtgtattctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGCGCCACGATCATGGGATCTCA 492 609 44 Antisense
ATGGGATCTCATCAACAAGTGGTTG 253 49 58 Antisense
CAAGTGGTTGTACCAGCCTACTCTG 18 185 73 Antisense
AGCCTACTACCCATCAGCATATGCT 90 83 99 Antisense
ATCAGCATATGCTTATCCATCAGTA 180 29 111 Antisense
GCTTATCCATCAGTATATTCTCCTT 466 305 121 Antisense
ATATTCTCCTTATGTATCTGCCTAT 451 19 135 Antisense
TCCTTATGTATCTGCCTATCCATCA 167 593 141 Antisense
TGCCTATCCATCAGTCTGGGCTTGG 335 585 153 Antisense
GTCTGGGCTTGGGGATCCAACAAGA 156 471 166 Antisense
AGGATGATGCTCCACGTGTATTCTC 383 57 194 Antisense
Sequence Source Affymetrix Proprietary Database