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[172468_x_at]

Description K01A2.9 /REP_DB=WormBase Gene ID /WP=CE19518 /TR=Q9TZ52 /GB=AAC69500.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 81      4grp_highest_8_8 > 40      4grp_high_8_8 > 42      6grp_highest_nonorm > 54      6grp_total > 91      4grp_total > 53      6grp_highest_sub > 05      6grp_high1_sub > 04      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1132
1 2 3 4 range
A 1248.3
(3)
P 		911.5
(1.1)
P 		687.8
(-0.1)
P 		168.5
(-3)
P 		1080
B 1170.2
(3)
P 		1089
(2.5)
P 		736.9
(0.5)
P 		115.9
(-3)
P 		1054
C 1227
(3)
P 		902.1
(1)
P 		596.3
(-0.9)
P 		258.4
(-3)
P 		969
avr 1215.2
(3) 		967.5
(1.6) 		673.7
(-0.1) 		180.9
(-3) 		1034

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1884
1 2 3 4 5 6 range
A 1108.4
(-1.1)
P 		1297.8
(-0.5)
P 		817.6
(-2.1)
P 		2320.2
(3)
P 		1893.9
(1.5)
P 		559.5
(-3)
P 		1761
B 1022.9
(-1.3)
P 		1126.5
(-0.9)
P 		607.6
(-2.7)
P 		2287.5
(3)
P 		1677.9
(0.9)
P 		514.8
(-3)
P 		1773
C 1150.6
(-0.3)
P 		1030.7
(-0.7)
P 		705.5
(-2)
P 		2008.7
(3)
P 		1492.9
(1)
P 		436.6
(-3)
P 		1572
avr 1094
(-0.9) 		1151.7
(-0.7) 		710.2
(-2.3) 		2205.5
(3) 		1688.2
(1.2) 		503.6
(-3) 		1702
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 430
1 2 3 4 range
A 454.5
(3)
P 		197.9
(-0.6)
P 		179.8
(-0.8)
P 		24.7
(-3)
P 		430
B 373.2
(3)
P 		269
(1.2)
P 		170.8
(-0.5)
P 		24.7
(-3)
P 		349
C 406.2
(3)
P 		259.4
(0.6)
P 		207.8
(-0.3)
P 		43.6
(-3)
P 		363
avr 411.3
(3) 		242.1
(0.3) 		186.1
(-0.6) 		31
(-3) 		380

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 546
1 2 3 4 5 6 range
A 204.3
(-2.1)
P 		461.6
(1.3)
P 		178
(-2.4)
P 		588.4
(3)
P 		571.5
(2.8)
P 		136.2
(-3)
P 		452
B 355.9
(-0.7)
P 		375
(-0.4)
P 		147.5
(-3)
P 		605.9
(2.1)
P 		681.7
(3)
P 		192
(-2.5)
P 		534
C 426.5
(2)
P 		314.5
(-0)
P 		179.6
(-2.4)
P 		404
(1.6)
P 		483.1
(3)
P 		146.2
(-3)
P 		337
avr 328.9
(-0.6) 		383.7
(0.2) 		168.4
(-2.9) 		532.8
(2.3) 		578.8
(3) 		158.1
(-3) 		421
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172468_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5072
Sequence Type Exemplar sequence
Representative Public ID K01A2.9 NCBI
Target Description K01A2.9 /REP_DB=WormBase Gene ID /WP=CE19518 /TR=Q9TZ52 /GB=AAC69500.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061303(9), NM_182229(9), NM_061306(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061303 NCBI Caenorhabditis elegans K01A2.8a (K01A2.8) mRNA, complete cds. 9/11 A 
NM_182229 NCBI Caenorhabditis elegans K01A2.8c (K01A2.8) mRNA, complete cds. 9/11 None
NM_061306 NCBI Caenorhabditis elegans K01A2.9 (K01A2.9) mRNA, complete cds. 11/11 None
GENEFINDER00000016841 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:309554:310570:-1 9/11 None
GENEFINDER00000016851 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:317593:318664:-1 11/11 None
GENEFINDER00000016852 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:315835:316906:-1 11/11 None
K01A2.8b ENSEMBL cdna:known chromosome:CEL140:II:309536:310570:-1 gene:K01A2.8 9/11 A 
K01A2.8c ENSEMBL cdna:known chromosome:CEL140:II:309540:310978:-1 gene:K01A2.8 9/11 A 
K01A2.8a ENSEMBL cdna:known chromosome:CEL140:II:309554:311349:-1 gene:K01A2.8 9/11 A 
K01A2.9 ENSEMBL cdna:known chromosome:CEL140:II:315889:316906:-1 gene:K01A2.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:315888-316906(-) 98.9 100.0
chrII:317646-318664(-) 98.9 100.0
chrII:309553-310570(-) 96.69 96.69
Public Domain and Genome References
Gene Title
Ensembl K01A2.8
K01A2.9
Entrez Gene 186820 Entrez gene
SwissProt Q9TZ52 EMBL-EBI
WormBase CE19518 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T33644 hypothetical protein K01A2.8b - Caenorhabditis elegans 1.0E-66
blast AAC69501 Mirp k channel accessory subunit protein 2, isoform a [Caenorhabditis elegans] ref|NP_493704.1| K01A2.8a [Caenorhabditis elegans] 4.0E-66
blast AAQ96604 MiRP4 [Caenorhabditis elegans] 6.0E-65
blast AAC69500 Hypothetical protein K01A2.9 [Caenorhabditis elegans] ref|NP_493707.1| K01A2.9 [Caenorhabditis elegans] 2.0E-61
blast AAC69500 Hypothetical protein K01A2.9 [Caenorhabditis elegans] ref|NP_493707.1| K01A2.9 [Caenorhabditis elegans] 1.0E-57
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493704.1 4 29-51,66-88,101-123,133-155
NP_872029.1 3 20-42,55-74,84-106
NP_493707.1 4 20-42,57-79,92-114,119-141
K01A2.8b 4 29-51,66-88,101-123,133-155
K01A2.8c 3 20-42,55-74,84-106
K01A2.8a 4 29-51,66-88,101-123,133-155
K01A2.9 4 20-42,57-79,92-114,119-141
GENEFINDER00000016841 4 29-51,66-88,101-123,133-155
GENEFINDER00000016851 4 20-42,57-79,92-114,124-146
GENEFINDER00000016852 4 20-42,57-79,92-114,124-146
Sequence
Target Sequence 
>C. ELEGANS:172468_X_AT
aaaaaagaatgtactcctgtccctgcactctctttcatctttaatccacgtggcaatgtt
ctatttcctattggtccaatggagtaaagtcgatattccagttatcacgcattttcagat
tttctgtctggtgattattggaatcggattattgattatcttgatcttatttttcattat
ttttaacaacgaaatgaaaagtgactcacatgcaagacgattctttcatatttggccact
ggtacatatcgccttatccaccgcctatcagctcttcacgcaaattgtgatatctcattg
gaaaacttaccagatcccaatcctctactcatcctgtgcaactaccgtctctgttggcat
tcattta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAAAGAATGTACTCCTGTCCCTG 27 131 63 Antisense
CTCTCTTTCATCTTTAATCCACGTG 70 231 90 Antisense
TCCACGTGGCAATGTTCTATTTCCT 250 587 107 Antisense
GTTCTATTTCCTATTGGTCCAATGG 15 445 120 Antisense
ATTCCAGTTATCACGCATTTTCAGA 656 7 157 Antisense
GTGACTCACATGCAAGACGATTCTT 304 483 263 Antisense
GACGATTCTTTCATATTTGGCCACT 177 379 278 Antisense
GGCCACTGGTACATATCGCCTTATC 209 541 296 Antisense
CCTATCAGCTCTTCACGCAAATTGT 436 269 326 Antisense
GGAAAACTTACCAGATCCCAATCCT 29 527 362 Antisense
TACCGTCTCTGTTGGCATTCATTTA 141 651 405 Antisense
Sequence Source Affymetrix Proprietary Database