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[172464_x_at]

Description F54D12.4 /REP_DB=WormBase Gene ID /WP=CE17869 /TR=O44828 /GB=AAB94994.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 18      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_total > 18      4grp_total > 36      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 93.4
(2.7)
P 		48.2
(-3)
P 		56
(-2)
P 		95.4
(3)
P 		47
B 72.2
(0.2)
P 		106.3
(3)
P 		33.3
(-3)
P 		81
(0.9)
P 		73
C 101.4
(2.1)
P 		112.3
(3)
P 		41.8
(-3)
P 		67.8
(-0.8)
P 		71
avr 89
(3) 		88.9
(3) 		43.7
(-3) 		81.4
(2) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 99.5
(3)
M 		67.6
(-1.1)
P 		52.9
(-3)
P 		64.7
(-1.5)
P 		79
(0.4)
P 		64.5
(-1.5)
P 		47
B 119.2
(3)
P 		84.1
(0.6)
P 		30.3
(-3)
A 		42
(-2.2)
P 		62.9
(-0.8)
P 		62.7
(-0.8)
P 		89
C 190.3
(3)
P 		91.1
(-1.5)
P 		59.3
(-3)
A 		86
(-1.8)
P 		86.6
(-1.7)
P 		87.4
(-1.7)
P 		131
avr 136.3
(3) 		80.9
(-0.7) 		47.5
(-3) 		64.2
(-1.9) 		76.2
(-1.1) 		71.5
(-1.4) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 34
(3)
P 		10.5
(-3)
P 		14.6
(-2)
P 		14
(-2.1)
P 		24
B 23
(1.9)
P 		26.3
(3)
P 		7.7
(-3)
P 		17.3
(0.1)
P 		19
C 33.6
(3)
P 		32.3
(2.6)
P 		14.6
(-2.1)
P 		11.4
(-3)
P 		22
avr 30.2
(3) 		23
(0.6) 		12.3
(-3) 		14.2
(-2.4) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 18.3
(0.3)
M 		24
(3)
P 		11.5
(-3)
P 		16.4
(-0.6)
P 		23.8
(2.9)
P 		15.7
(-1)
P 		13
B 41.5
(3)
P 		28
(0.6)
P 		7.4
(-3)
A 		11.1
(-2.3)
P 		25.6
(0.2)
P 		23.4
(-0.2)
P 		34
C 70.5
(3)
P 		27.8
(-1.6)
P 		15.1
(-3)
A 		17.3
(-2.8)
P 		28
(-1.6)
P 		29.3
(-1.5)
P 		55
avr 43.4
(3) 		26.6
(-0.1) 		11.3
(-3) 		14.9
(-2.3) 		25.8
(-0.3) 		22.8
(-0.9) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172464_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4708
Sequence Type Exemplar sequence
Representative Public ID F54D12.4 NCBI
Target Description F54D12.4 /REP_DB=WormBase Gene ID /WP=CE17869 /TR=O44828 /GB=AAB94994.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061555(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061555 NCBI Caenorhabditis elegans F54D12.4 (F54D12.4) mRNA, complete cds. 11/11 None
F54D12.4 ENSEMBL cdna:known chromosome:CEL140:II:1368096:1368305:-1 gene:F54D12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1368094-1368304(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D12.4
Entrez Gene 173515 Entrez gene
SwissProt O44828 EMBL-EBI
WormBase CE17869 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94994 Hypothetical protein F54D12.4 [Caenorhabditis elegans] ref|NP_493956.1| F54D12.4 [Caenorhabditis elegans] 4.0E-19
Sequence
Target Sequence 
>C. ELEGANS:172464_X_AT
atgtccaccaattcgtttgtttcatccttttttatcgccatcccccaaccagttttttgt
caacgagccaattttttgtgctatatattgaactctgatatgatgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCCACCAATTCGTTTGTTTCAT 608 47 13 Antisense
GTCCACCAATTCGTTTGTTTCATCC 627 475 15 Antisense
CACCAATTCGTTTGTTTCATCCTTT 700 201 18 Antisense
TCGTTTGTTTCATCCTTTTTTATCG 198 605 25 Antisense
TTTGTTTCATCCTTTTTTATCGCCA 113 667 28 Antisense
CAACCAGTTTTTTGTCAACGAGCCA 492 185 58 Antisense
TTTGTCAACGAGCCAATTTTTTGTG 95 615 68 Antisense
AACGAGCCAATTTTTTGTGCTATAT 310 145 74 Antisense
TTTGTGCTATATATTGAACTCTGAT 399 667 87 Antisense
GTGCTATATATTGAACTCTGATATG 699 479 90 Antisense
TATATTGAACTCTGATATGATGAAT 421 633 96 Antisense
Sequence Source Affymetrix Proprietary Database