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[172447_at]

Description M03D4.3 /REP_DB=WormBase Gene ID /WP=CE12350 /TR=Q21491 /GB=AAC24405.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 41      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 32      6grp_total > 31      4grp_total > 04      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2509
1 2 3 4 range
A 1060.2
(-1.8)
P 		744.6
(-2.6)
P 		609.6
(-3)
P 		2847.5
(3)
P 		2238
B 1359.8
(-1.2)
P 		622.6
(-3)
P 		716.1
(-2.8)
P 		3102.6
(3)
P 		2480
C 1183.3
(-1.5)
P 		722.9
(-2.7)
P 		593.3
(-3)
P 		2981.5
(3)
P 		2388
avr 1201.1
(-1.6) 		696.7
(-2.9) 		639.7
(-3) 		2977.2
(3) 		2338

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1403
1 2 3 4 5 6 range
A 1106.4
(-1)
P 		1226.7
(-0.5)
P 		2048.5
(3)
P 		1146.8
(-0.9)
P 		1010
(-1.4)
P 		645.3
(-3)
P 		1403
B 1309.4
(0.7)
P 		1484.3
(2.2)
P 		1584.1
(3)
P 		1098.8
(-1)
P 		1275.1
(0.5)
P 		856
(-3)
P 		728
C 1155.1
(-0.1)
P 		1351.9
(2.1)
P 		1000.3
(-1.9)
P 		1430.2
(3)
P 		901.1
(-3)
P 		1019.2
(-1.7)
P 		529
avr 1190.3
(-0) 		1354.3
(1.4) 		1544.3
(3) 		1225.3
(0.3) 		1062.1
(-1.1) 		840.2
(-3) 		704
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 507
1 2 3 4 range
A 386
(2.3)
P 		161.6
(-2.9)
P 		159.4
(-3)
P 		416.8
(3)
P 		257
B 433.6
(0.3)
P 		153.8
(-3)
P 		165.9
(-2.9)
P 		661.2
(3)
P 		507
C 391.7
(0.7)
P 		207.8
(-3)
P 		206.8
(-3)
P 		502.7
(3)
P 		296
avr 403.8
(0.9) 		174.4
(-3) 		177.4
(-2.9) 		526.9
(3) 		353

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 361
1 2 3 4 5 6 range
A 204
(-2)
P 		436.3
(2.8)
P 		446.1
(3)
P 		290.9
(-0.2)
P 		304.8
(0.1)
P 		157.1
(-3)
P 		289
B 455.6
(1.3)
P 		494.1
(2.4)
P 		384.7
(-0.5)
P 		291
(-3)
P 		518.1
(3)
P 		319.2
(-2.3)
P 		227
C 428.1
(3)
P 		412.5
(2.5)
P 		254.6
(-3)
P 		287.6
(-1.9)
P 		291.6
(-1.7)
P 		341.2
(-0)
P 		174
avr 362.6
(0.1) 		447.6
(3) 		361.8
(0.1) 		289.8
(-2.4) 		371.5
(0.4) 		272.5
(-3) 		175
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172447_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10678
Sequence Type Exemplar sequence
Representative Public ID M03D4.3 NCBI
Target Description M03D4.3 /REP_DB=WormBase Gene ID /WP=CE12350 /TR=Q21491 /GB=AAC24405.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068516(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068516 NCBI Caenorhabditis elegans M03D4.3 (M03D4.3) mRNA, complete cds. 11/11 None
M03D4.3 ENSEMBL cdna:known chromosome:CEL140:IV:6110693:6112356:-1 gene:M03D4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6110698-6112362(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M03D4.3
Entrez Gene 187421 Entrez gene
SwissProt Q21491 EMBL-EBI
WormBase CE12350 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24405 Hypothetical protein M03D4.3 [Caenorhabditis elegans] ref|NP_500917.1| M03D4.3 [Caenorhabditis elegans] 2.0E-27
blast CAE61602 Hypothetical protein CBG05521 [Caenorhabditis briggsae] 8.0E-25
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500917.1 1 4-26
M03D4.3 1 4-26
Sequence
Target Sequence 
>C. ELEGANS:172447_AT
cttccaagtcctcttgcttgttgttctcatctctgccaccgtggctcttccatttttcaa
ctcgccaactagccaaacatatggcggacaatggcgtcaaatgtacaccgacgagaatct
ggaagttccacaaaatccgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCCAAGTCCTCTTGCTTGTTGTT 459 219 21 Antisense
TCCAAGTCCTCTTGCTTGTTGTTCT 282 587 23 Antisense
ATTTTTCAACTCGCCAACTAGCCAA 454 13 72 Antisense
TTCAACTCGCCAACTAGCCAAACAT 247 685 76 Antisense
AACTCGCCAACTAGCCAAACATATG 66 143 79 Antisense
GCCAACTAGCCAAACATATGGCGGA 133 277 84 Antisense
AACATATGGCGGACAATGGCGTCAA 57 139 96 Antisense
GACAATGGCGTCAAATGTACACCGA 132 365 107 Antisense
AAATGTACACCGACGAGAATCTGGA 507 119 119 Antisense
ACCGACGAGAATCTGGAAGTTCCAC 212 89 127 Antisense
TCTGGAAGTTCCACAAAATCCGAGA 52 599 138 Antisense
Sequence Source Affymetrix Proprietary Database