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[172433_x_at]

Description F40E3.3 /REP_DB=WormBase Gene ID /WP=CE10162 /TR=O01548 /GB=AAC24294.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 81      4grp_total > 43      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 46.7
(1.4)
A 		47.4
(1.7)
A 		50.8
(3)
A 		35.3
(-3)
A 		16
B 9.7
(-3)
A 		80
(3)
M 		10.5
(-2.9)
A 		11.9
(-2.8)
A 		70
C 38.5
(-1.3)
A 		64.9
(3)
A 		27.9
(-3)
A 		43.3
(-0.5)
A 		37
avr 31.6
(-2.7) 		64.1
(3) 		29.7
(-3) 		30.2
(-2.9) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 48.6
(-0.6)
A 		30.1
(-3)
A 		36.6
(-2.2)
P 		61
(1)
P 		76.7
(3)
A 		53.3
(-0)
A 		47
B 47.4
(0.5)
P 		62.6
(3)
A 		39.1
(-0.9)
A 		36.9
(-1.2)
A 		43
(-0.2)
A 		26
(-3)
A 		37
C 28.1
(-2.1)
A 		42.1
(0.5)
A 		54.8
(2.9)
A 		55.4
(3)
A 		23.3
(-3)
A 		32.9
(-1.2)
A 		32
avr 41.4
(-1.2) 		44.9
(0.3) 		43.5
(-0.3) 		51.1
(3) 		47.7
(1.5) 		37.4
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 17
(3)
A 		10.3
(-0.4)
A 		13.3
(1.1)
A 		5.2
(-3)
A 		12
B 3.1
(-2.8)
A 		19.8
(3)
M 		2.4
(-3)
A 		2.5
(-3)
A 		17
C 12.7
(-0.2)
A 		18.7
(3)
A 		9.7
(-1.7)
A 		7.3
(-3)
A 		11
avr 10.9
(0.1) 		16.3
(3) 		8.5
(-1.1) 		5
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 9
(-2.6)
A 		10.7
(-1.9)
A 		8
(-3)
P 		15.5
(-0)
P 		23.1
(3)
A 		13
(-1)
A 		15
B 16.5
(0.7)
P 		20.9
(3)
A 		9.5
(-3)
A 		9.8
(-2.8)
A 		17.5
(1.2)
A 		9.7
(-2.9)
A 		11
C 10.4
(-0.3)
A 		12.9
(2.1)
A 		13.9
(3)
A 		11.2
(0.5)
A 		7.5
(-3)
A 		11
(0.3)
A 		6
avr 12
(-1.4) 		14.8
(1.7) 		10.5
(-3) 		12.2
(-1.1) 		16
(3) 		11.2
(-2.2) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172433_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.214
Sequence Type Exemplar sequence
Representative Public ID F40E3.3 NCBI
Target Description F40E3.3 /REP_DB=WormBase Gene ID /WP=CE10162 /TR=O01548 /GB=AAC24294.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058616(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058616 NCBI Caenorhabditis elegans F40E3.3 (F40E3.3) mRNA, complete cds. 11/11 None
GENEFINDER00000025939 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2638650:2639314:1 11/11 None
F40E3.3 ENSEMBL cdna:known chromosome:CEL140:I:2638650:2639314:1 gene:F40E3.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067584 3/11 Cross Hyb Matching Probes A 
GENEFINDER00000009692 1/11 Cross Hyb Matching Probes None
GENEFINDER00000041573 3/11 Cross Hyb Matching Probes None
GENEFINDER00000009127 2/11 Cross Hyb Matching Probes None
GENEFINDER00000016876 2/11 Cross Hyb Matching Probes None
F56B3.3 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2638649-2639314(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F40E3.3
Entrez Gene 185533 Entrez gene
SwissProt O01548 EMBL-EBI
WormBase CE10162 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24294 Hypothetical protein F40E3.3 [Caenorhabditis elegans] ref|NP_491017.1| F40E3.3 [Caenorhabditis elegans] 5.0E-24
Sequence
Target Sequence 
>C. ELEGANS:172433_X_AT
aattctaatattccaccacattcgccatcttcatcagcttcttcatctcgaactcctgga
tttcctacaaaaatcggatttttttcctctatatcgtccaccacgttcgccatcttcatc
gttatcttcgtctccagctcctggattttgcagaaaattcggatttttttctctatgtca
gtcagatttatatatttcatcaccaccaacatcttcatccacttcttcgcctgaaacgac
gggattttccacaaaaatcggattttttctcttgatagtccacctccaccgccttcttca
tccacttcttcgtctcgaactcctagattttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTCTAATATTCCACCACATTCGC 456 175 57 Antisense
ATCTCGAACTCCTGGATTTCCTACA 446 23 101 Antisense
ATCGGATTTTTTTCCTCTATATCGT 6 35 129 Antisense
TCGTCTCCAGCTCCTGGATTTTGCA 179 605 184 Antisense
AATTCGGATTTTTTTCTCTATGTCA 613 177 212 Antisense
TTTCTCTATGTCAGTCAGATTTATA 503 671 224 Antisense
ATATATTTCATCACCACCAACATCT 444 19 246 Antisense
CCACTTCTTCGCCTGAAACGACGGG 705 269 275 Antisense
GCCTGAAACGACGGGATTTTCCACA 440 281 285 Antisense
GATTTTTTCTCTTGATAGTCCACCT 381 429 317 Antisense
TTCGTCTCGAACTCCTAGATTTTGC 654 695 365 Antisense
Sequence Source Affymetrix Proprietary Database