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[172431_x_at]

Description H12D21.1 /REP_DB=WormBase Gene ID /WP=CE15223 /TR=Q23303 /GB=CAB07428.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 03      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1718
1 2 3 4 range
A 156.5
(-2.5)
A 		5.8
(-3)
A 		5.6
(-3)
A 		1720.1
(3)
P 		1715
B 175.3
(-2)
P 		36.9
(-2.9)
A 		11.3
(-3)
A 		1046.6
(3)
P 		1035
C 173.2
(-2.4)
A 		7.8
(-3)
A 		2.6
(-3)
A 		1646.9
(3)
P 		1644
avr 168.3
(-2.3) 		16.8
(-3) 		6.5
(-3) 		1471.2
(3) 		1465

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5335
1 2 3 4 5 6 range
A 36.7
(-3)
A 		65.8
(-3)
A 		270.3
(-2.7)
P 		1873.6
(-0.6)
P 		3058.3
(0.9)
P 		4642.8
(3)
P 		4606
B 80.8
(-3)
A 		89.4
(-3)
A 		116.3
(-3)
A 		1612.5
(-1.1)
P 		3935.1
(1.8)
P 		4861.5
(3)
P 		4781
C 99.8
(-3)
A 		118.2
(-3)
A 		107
(-3)
A 		1644.9
(-1.2)
P 		3491.6
(0.9)
P 		5372.1
(3)
P 		5272
avr 72.4
(-3) 		91.1
(-3) 		164.5
(-2.9) 		1710.3
(-1) 		3495
(1.2) 		4958.8
(3) 		4886
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 277
1 2 3 4 range
A 57
(-1.7)
A 		1.2
(-3)
A 		1.5
(-3)
A 		251.8
(3)
P 		251
B 55.9
(-1.5)
P 		9.1
(-2.8)
A 		2.6
(-3)
A 		223
(3)
P 		220
C 57.3
(-1.8)
A 		2.2
(-3)
A 		0.9
(-3)
A 		277.7
(3)
P 		277
avr 56.7
(-1.7) 		4.2
(-2.9) 		1.7
(-3) 		250.8
(3) 		249

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1806
1 2 3 4 5 6 range
A 6.8
(-3)
A 		23.4
(-2.9)
A 		58.9
(-2.7)
P 		475.2
(-0.5)
P 		922.9
(1.9)
P 		1130.4
(3)
P 		1124
B 28.1
(-3)
A 		29.8
(-3)
A 		28.3
(-3)
A 		427.1
(-1.7)
P 		1598.8
(2.3)
P 		1812.8
(3)
P 		1785
C 37
(-3)
A 		36.1
(-3)
A 		27.2
(-3)
A 		330.8
(-2)
P 		1129.8
(0.7)
P 		1798.3
(3)
P 		1771
avr 24
(-3) 		29.8
(-3) 		38.1
(-2.9) 		411
(-1.5) 		1217.2
(1.6) 		1580.5
(3) 		1557
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172431_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13909
Sequence Type Exemplar sequence
Representative Public ID H12D21.1 NCBI
Target Description H12D21.1 /REP_DB=WormBase Gene ID /WP=CE15223 /TR=Q23303 /GB=CAB07428.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074253(11), NM_074263(11), NM_001028836(11), NM_074268(11), NM_001028837(11), NM_074265(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074253 NCBI Caenorhabditis elegans Nematode Specific Peptide family/group A family member (nspa-8) (nspa-8) mRNA, complete cds. 11/11 None
NM_074263 NCBI Caenorhabditis elegans Nematode Specific Peptide family/group A family member (nspa-5) (nspa-5) mRNA, complete cds. 11/11 None
NM_001028836 NCBI Caenorhabditis elegans Nematode Specific Peptide family/group A family member (nspa-2) (nspa-2) mRNA, complete cds. 11/11 None
NM_074268 NCBI Caenorhabditis elegans Nematode Specific Peptide family/group A family member (nspa-1) (nspa-1) mRNA, complete cds. 11/11 None
NM_001028837 NCBI Caenorhabditis elegans Nematode Specific Peptide family/group A family member (nspa-4) (nspa-4) mRNA, complete cds. 11/11 None
NM_074265 NCBI Caenorhabditis elegans Nematode Specific Peptide family/group A family member (nspa-3) (nspa-3) mRNA, complete cds. 11/11 None
GENEFINDER00000018100 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14883024:14884314:-1 11/11 None
GENEFINDER00000026982 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14889392:14891066:-1 11/11 None
W06A7.5 ENSEMBL cdna:known chromosome:CEL140:V:14829537:14829641:-1 gene:W06A7.5 11/11 None
ZC412.6 ENSEMBL cdna:known chromosome:CEL140:V:14880937:14881515:1 gene:ZC412.6 11/11 None
ZC412.7 ENSEMBL cdna:known chromosome:CEL140:V:14883024:14883128:-1 gene:ZC412.7 11/11 None
H12D21.1 ENSEMBL cdna:known chromosome:CEL140:V:14889392:14889496:-1 gene:H12D21.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028838 7/11 Cross Hyb Matching Probes None
NM_001028839 7/11 Cross Hyb Matching Probes None
NM_001029084 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14887304-14887409(+) 99.05 99.05
chrV:14880936-14881041(+) 99.05 99.05
chrV:14893670-14893775(+) 98.1 98.1
chrI:2350846-2350946(+) 91.43 91.43
chrV:14889391-14889496(-) 100.0 100.0
chrV:14883023-14883128(-) 100.0 100.0
chrV:14891995-14892100(-) 98.1 98.1
chrV:14885627-14885732(-) 98.1 98.1
chrV:14829545-14829641(-) 90.48 90.48
Public Domain and Genome References
Gene Title





Gene Symbol nspa-1
nspa-2
nspa-4
nspa-8
nspa-5
nspa-3
Ensembl W06A7.5
ZC412.6
ZC412.7
H12D21.1
Entrez Gene 179980 Entrez gene
179983 Entrez gene
179985 Entrez gene
191162 Entrez gene
3564808 Entrez gene
3565045 Entrez gene
SwissProt Q23303 EMBL-EBI
Q23306 EMBL-EBI
Q23184 EMBL-EBI
WormBase CE15223 Wormbase
CE15223 Wormbase
CE15221 Wormbase
CE14672 Wormbase
CE15221 Wormbase
CE15223 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB63209 Hypothetical protein H12D21.2 [Caenorhabditis elegans] ref|NP_506670.2| H12D21.2 [Caenorhabditis elegans] 7.0E-72
blast CAB01525 Hypothetical protein ZC412.9 [Caenorhabditis elegans] ref|NP_506667.1| ZC412.9 [Caenorhabditis elegans] 3.0E-38
blast CAB63209 Hypothetical protein H12D21.2 [Caenorhabditis elegans] ref|NP_506670.2| H12D21.2 [Caenorhabditis elegans] 3.0E-38
blast CAB01525 Hypothetical protein ZC412.9 [Caenorhabditis elegans] ref|NP_506667.1| ZC412.9 [Caenorhabditis elegans] 7.0E-38
blast CAB01529 Hypothetical protein ZC412.6 [Caenorhabditis elegans] emb|CAI79205.1| Hypothetical protein H12D21.13 [Caenorhabditis elegans] ref|NP_001024008.1| Nematode Specific Peptide family/group A family member (nspa-4) [Caenorhabditis elegans] ref|NP_506664.1| Nematode Specific Peptide family/group A family member (nspa-5) [Caenorhabditis elegans] 4.0E-11
blast CAB01526 Hypothetical protein ZC412.7 [Caenorhabditis elegans] emb|CAI79204.1| Hypothetical protein H12D21.12 [Caenorhabditis elegans] emb|CAB07428.1| Hypothetical protein H12D21.1 [Caenorhabditis elegans] ref|NP_001024007.1| Nematode Specific Peptide family/group A family member (nspa-2) [Caenorhabditis elegans] ref|NP_506666.1| Nematode Specific Peptide family/group A family member (nspa-3) [Caenorhabditis elegans] ref|NP_506669.1| Nematode Specific Peptide family/group A family member (nspa-1) [Caenorhabditis elegans] 7.0E-11
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506654.1 1 7-29
NP_506664.1 1 7-29
NP_001024007.1 1 4-26
NP_506669.1 1 4-26
NP_001024008.1 1 7-29
NP_506666.1 1 4-26
W06A7.5 1 7-29
ZC412.6 1 7-29
ZC412.7 1 4-26
H12D21.1 1 4-26
GENEFINDER00000018100 2 132-154,158-180
GENEFINDER00000026982 3 5-27,206-228,232-254
Sequence
Target Sequence 
>C. ELEGANS:172431_X_AT
tctcgcctacactgcaatggcttggattggactcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCGCCTACACTGCAATGGCTTGG 422 609 51 Antisense
CTCGCCTACACTGCAATGGCTTGGA 620 233 52 Antisense
TCGCCTACACTGCAATGGCTTGGAT 458 597 53 Antisense
CGCCTACACTGCAATGGCTTGGATT 373 257 54 Antisense
GCCTACACTGCAATGGCTTGGATTG 421 281 55 Antisense
CCTACACTGCAATGGCTTGGATTGG 641 265 56 Antisense
TACACTGCAATGGCTTGGATTGGAC 103 641 58 Antisense
CACTGCAATGGCTTGGATTGGACTC 6 197 60 Antisense
ACTGCAATGGCTTGGATTGGACTCA 632 97 61 Antisense
CTGCAATGGCTTGGATTGGACTCAG 43 239 62 Antisense
TGCAATGGCTTGGATTGGACTCAGC 153 577 63 Antisense
Sequence Source Affymetrix Proprietary Database