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[172427_x_at]

Description B0213.3 /REP_DB=WormBase Gene ID /WP=CE16774 /TR=O44665 /GB=AAC47932.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 20      6grp_total > 17      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 320
1 2 3 4 range
A 646.8
(3)
P 		630.5
(2.7)
P 		574.2
(1.5)
P 		362.5
(-3)
P 		284
B 462.1
(-3)
P 		591.3
(3)
P 		568.5
(1.9)
P 		478
(-2.3)
P 		129
C 654.7
(2.2)
P 		614.6
(1.1)
P 		682.6
(3)
P 		462.6
(-3)
P 		220
avr 587.9
(2.2) 		612.1
(3) 		608.4
(2.9) 		434.4
(-3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1669
1 2 3 4 5 6 range
A 1442.4
(3)
P 		1136
(1.1)
P 		983.9
(0.1)
P 		661.2
(-1.9)
P 		488.3
(-3)
P 		661.8
(-1.9)
P 		954
B 1817.9
(3)
P 		1059.6
(-0.3)
P 		885.7
(-1)
P 		418.2
(-3)
P 		750.9
(-1.6)
P 		593.1
(-2.3)
P 		1400
C 2086.8
(3)
P 		941.9
(-1.4)
P 		834.9
(-1.8)
P 		512.2
(-3)
P 		827.3
(-1.8)
P 		659.9
(-2.4)
P 		1575
avr 1782.4
(3) 		1045.8
(-0.5) 		901.5
(-1.2) 		530.5
(-3) 		688.8
(-2.2) 		638.3
(-2.5) 		1252
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 235.5
(3)
P 		136.9
(-0.2)
P 		150.1
(0.2)
P 		53.1
(-3)
P 		182
B 147.4
(3)
P 		146
(2.8)
P 		131.7
(0.9)
P 		101.9
(-3)
P 		46
C 216.7
(2.2)
P 		176.7
(0.7)
P 		237.9
(3)
P 		78
(-3)
P 		160
avr 199.9
(3) 		153.2
(0.7) 		173.2
(1.7) 		77.7
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 671
1 2 3 4 5 6 range
A 265.9
(-0.2)
P 		404
(3)
P 		214.3
(-1.4)
P 		167.7
(-2.5)
P 		147.3
(-3)
P 		161.1
(-2.7)
P 		257
B 632.5
(3)
P 		352.7
(-0.2)
P 		215.1
(-1.8)
P 		110.8
(-3)
P 		305.1
(-0.8)
P 		221.2
(-1.7)
P 		522
C 773.5
(3)
P 		287.4
(-1.3)
P 		212.5
(-2)
P 		103
(-3)
P 		267.7
(-1.5)
P 		220.9
(-1.9)
P 		671
avr 557.3
(3) 		348
(0.1) 		214
(-1.8) 		127.2
(-3) 		240
(-1.4) 		201.1
(-2) 		430
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172427_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15841
Sequence Type Exemplar sequence
Representative Public ID B0213.3 NCBI
Target Description B0213.3 /REP_DB=WormBase Gene ID /WP=CE16774 /TR=O44665 /GB=AAC47932.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071709(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071709 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-28) (nlp-28) mRNA, complete cds. 11/11 None
SNAP00000025862 ENSEMBL cdna:SNAP chromosome:CEL140:V:3987747:3988026:1 11/11 None
GENEFINDER00000025875 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3987747:3988026:1 11/11 None
B0213.3 ENSEMBL cdna:known chromosome:CEL140:V:3987747:3988026:1 gene:B0213.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071707 2/11 Cross Hyb Matching Probes A 
NM_071706 2/11 Cross Hyb Matching Probes A 
NM_071708 3/11 Cross Hyb Matching Probes A 
SNAP00000025859 1/11 Cross Hyb Matching Probes None
SNAP00000025860 2/11 Cross Hyb Matching Probes None
SNAP00000025861 3/11 Cross Hyb Matching Probes None
GENEFINDER00000025869 2/11 Cross Hyb Matching Probes None
GENEFINDER00000025870 1/11 Cross Hyb Matching Probes None
GENEFINDER00000025873 3/11 Cross Hyb Matching Probes None
B0213.6 2/11 Cross Hyb Matching Probes A 
B0213.5 2/11 Cross Hyb Matching Probes A 
B0213.4 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3987746-3988026(+) 100.0 100.0
chrV:3982194-3984549(+) 93.43 93.43
Public Domain and Genome References
Gene Title
Gene Symbol nlp-28
Ensembl B0213.3
Entrez Gene 181845 Entrez gene
SwissProt O44665 EMBL-EBI
WormBase CE16774 Wormbase
Functional Annotations
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504110.1 1 7-29
B0213.3 1 7-29
SNAP00000025862 1 7-29
GENEFINDER00000025875 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:172427_X_AT
atgatttcaacttcttcaattctgattctcgtcttccttctcgcttgcttcatggctacc
agtgctcagtggggatatggaggatatggaagaggttatggtggatatggtggatacgga
cgtggaatgtatggaggctatggacgcggaatgtatggtggatatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATTTCAACTTCTTCAATTCTGA 238 47 13 Antisense
CTTCTTCAATTCTGATTCTCGTCTT 329 221 23 Antisense
CTTCATGGCTACCAGTGCTCAGTGG 182 219 60 Antisense
CATGGCTACCAGTGCTCAGTGGGGA 576 209 63 Antisense
GGCTACCAGTGCTCAGTGGGGATAT 208 537 66 Antisense
ACCAGTGCTCAGTGGGGATATGGAG 489 85 70 Antisense
GGAAGAGGTTATGGTGGATATGGTG 372 531 100 Antisense
GGATATGGTGGATACGGACGTGGAA 258 509 115 Antisense
GGACGTGGAATGTATGGAGGCTATG 262 523 130 Antisense
GGAGGCTATGGACGCGGAATGTATG 571 513 145 Antisense
GGACGCGGAATGTATGGTGGATATG 263 523 154 Antisense
Sequence Source Affymetrix Proprietary Database