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[172417_x_at]

Description F38A5.5 /REP_DB=WormBase Gene ID /WP=CE10034 /TR=Q94212 /GB=AAB09149.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 91      4grp_high_8_8 > 55      6grp_total > 83      4grp_total > 48      6grp_cgc > 98      6grp_high1_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 924
1 2 3 4 range
A 94.5
(-3)
P 		404.2
(-0.8)
P 		927.2
(3)
P 		119
(-2.8)
P 		833
B 119.3
(-2.7)
P 		512.7
(-0.1)
P 		989.8
(3)
P 		65.5
(-3)
A 		924
C 100
(-3)
A 		506.2
(0)
P 		901.6
(3)
P 		125.7
(-2.8)
M 		802
avr 104.6
(-3) 		474.4
(-0.3) 		939.5
(3) 		103.4
(-3) 		836

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 525
1 2 3 4 5 6 range
A 94.9
(-2.2)
A 		81.5
(-2.4)
M 		243.6
(-0.5)
P 		251
(-0.4)
P 		548.9
(3)
P 		25.8
(-3)
A 		523
B 97.6
(-2.8)
P 		154.3
(-2.1)
P 		303
(-0.2)
P 		273.4
(-0.5)
P 		551
(3)
P 		81.7
(-3)
P 		469
C 72.2
(-3)
P 		194.8
(-0.1)
P 		109.6
(-2.1)
A 		272.3
(1.8)
P 		322.8
(3)
P 		71.8
(-3)
P 		251
avr 88.2
(-2.6) 		143.5
(-1.8) 		218.7
(-0.7) 		265.6
(-0) 		474.2
(3) 		59.8
(-3) 		414
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 300
1 2 3 4 range
A 34.4
(-2.5)
P 		87.7
(-1.1)
P 		242.4
(3)
P 		17.4
(-3)
P 		225
B 38
(-2.3)
P 		126.6
(0.1)
P 		229.4
(3)
P 		14
(-3)
A 		215
C 33.1
(-2.8)
A 		145.5
(-0.5)
P 		314.2
(3)
P 		21.2
(-3)
M 		293
avr 35.2
(-2.6) 		119.9
(-0.5) 		262
(3) 		17.5
(-3) 		245

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 17.5
(-2.6)
A 		29
(-2.1)
M 		53.1
(-1.2)
P 		63.7
(-0.8)
P 		165.6
(3)
P 		6.3
(-3)
A 		159
B 33.9
(-2.9)
P 		51.4
(-2.4)
P 		73.6
(-1.7)
P 		72.4
(-1.7)
P 		223.8
(3)
P 		30.5
(-3)
P 		193
C 26.8
(-2.8)
P 		59.4
(-0.4)
P 		27.9
(-2.7)
A 		54.8
(-0.7)
P 		104.4
(3)
P 		24
(-3)
P 		80
avr 26.1
(-2.8) 		46.6
(-1.9) 		51.5
(-1.7) 		63.6
(-1.2) 		164.6
(3) 		20.3
(-3) 		144
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172417_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10444
Sequence Type Exemplar sequence
Representative Public ID F38A5.5 NCBI
Target Description F38A5.5 /REP_DB=WormBase Gene ID /WP=CE10034 /TR=Q94212 /GB=AAB09149.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068609(11), NM_068607(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068609 NCBI Caenorhabditis elegans Nematode Specific Peptide family, group B family member (nspb-3) (nspb-3) mRNA, complete cds. 11/11 A 
NM_068607 NCBI Caenorhabditis elegans Nematode Specific Peptide family, group B family member (nspb-2) (nspb-2) mRNA, complete cds. 10/11 A 
SNAP00000010566 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6591727:6592002:1 9/11 None
SNAP00000010568 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6594639:6594914:-1 10/11 None
GENEFINDER00000010577 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6591703:6592002:1 10/11 None
GENEFINDER00000010587 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6594639:6594938:-1 11/11 None
F38A5.12 ENSEMBL cdna:known chromosome:CEL140:IV:6591703:6592002:1 gene:F38A5.12 10/11 A 
F38A5.5 ENSEMBL cdna:known chromosome:CEL140:IV:6594639:6594938:-1 gene:F38A5.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068612 6/11 Cross Hyb Matching Probes A 
NM_068611 7/11 Cross Hyb Matching Probes A 
NM_001028019 7/11 Cross Hyb Matching Probes None
SNAP00000010565 6/11 Cross Hyb Matching Probes None
SNAP00000010571 6/11 Cross Hyb Matching Probes None
SNAP00000010572 5/11 Cross Hyb Matching Probes None
GENEFINDER00000010579 7/11 Cross Hyb Matching Probes None
GENEFINDER00000010584 6/11 Cross Hyb Matching Probes None
GENEFINDER00000010586 7/11 Cross Hyb Matching Probes None
F38A5.14 4/11 Cross Hyb Matching Probes None
F38A5.10 7/11 Cross Hyb Matching Probes A 
F38A5.9 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6591708-6592008(+) 99.6 99.6
chrIV:6598223-6598521(+) 96.79 96.79
chrIV:6599187-6599478(+) 95.58 95.58
chrIV:6594644-6594944(-) 100.0 100.0
chrIV:6590785-6591083(-) 97.59 97.59
Public Domain and Genome References
Gene Title

Gene Symbol nspb-2
nspb-3
Ensembl F38A5.12
F38A5.5
Entrez Gene 185443 Entrez gene
185448 Entrez gene
SwissProt Q94214 EMBL-EBI
Q94212 EMBL-EBI
WormBase CE10048 Wormbase
CE10034 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB09149 Nematode specific peptide family, group b protein 3 [Caenorhabditis elegans] ref|NP_501010.1| Nematode Specific Peptide family, group B family member (nspb-3) [Caenorhabditis elegans] 2.0E-20
blast AAB09156 Nematode specific peptide family, group b protein 2 [Caenorhabditis elegans] ref|NP_501008.1| Nematode Specific Peptide family, group B family member (nspb-2) [Caenorhabditis elegans] 2.0E-20
blast AAQ01533 Nematode specific peptide family, group b protein 1 [Caenorhabditis elegans] ref|NP_001023190.1| Nematode Specific Peptide family, group B family member (nspb-1) [Caenorhabditis elegans] 3.0E-20
blast T29894 hypothetical protein F38A5.3 - Caenorhabditis elegans 3.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR009924 EMBL-EBI Protein of unknown function DUF1459 1.5E-62
Pfam IPR009924 EMBL-EBI Protein of unknown function DUF1459 3.5E-61
Pfam IPR009924 EMBL-EBI Protein of unknown function DUF1459 4.9E-53
Pfam IPR009924 EMBL-EBI Protein of unknown function DUF1459 1.0E-51
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501010.1 2 7-24,39-61
NP_501008.1 2 7-24,39-61
F38A5.12 2 7-24,39-61
F38A5.5 2 7-24,39-61
GENEFINDER00000010577 2 7-24,39-61
GENEFINDER00000010587 2 7-24,39-61
Sequence
Target Sequence 
>C. ELEGANS:172417_X_AT
attgtcatgatttttgcccttttctgtatttcatcgtgtcaagttatatatactcctgaa
gttgtatcatctccatattattatgcttcatctccagttgcttctgcttatccatatgct
tatgcttatggagctgctgcttatccaactgcttattacggatggggatctaatatggga
caacaagcaccatcttctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGTCATGATTTTTGCCCTTTTCT 382 5 31 Antisense
CCCTTTTCTGTATTTCATCGTGTCA 640 259 47 Antisense
GTGTCAAGTTATATATACTCCTGAA 522 485 66 Antisense
TACTCCTGAAGTTGTATCATCTCCA 113 647 81 Antisense
GTATCATCTCCATATTATTATGCTT 185 455 94 Antisense
TATTATGCTTCATCTCCAGTTGCTT 693 661 109 Antisense
AGTTGCTTCTGCTTATCCATATGCT 312 65 126 Antisense
ATGCTTATGGAGCTGCTGCTTATCC 449 43 152 Antisense
GCTGCTTATCCAACTGCTTATTACG 557 295 166 Antisense
AACTGCTTATTACGGATGGGGATCT 57 143 177 Antisense
TGGGACAACAAGCACCATCTTCTGC 395 553 206 Antisense
Sequence Source Affymetrix Proprietary Database