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[172401_x_at]

Description F08B4.3 /REP_DB=WormBase Gene ID /WP=CE07044 /TR=Q19193 /GB=AAB37726.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 85      6grp_total > 88      4grp_total > 13      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 42.6
(-0.4)
P 		15
(-3)
A 		19.7
(-2.6)
A 		79.9
(3)
P 		65
B 37.5
(-0.8)
P 		23.3
(-2)
A 		10.6
(-3)
A 		84.1
(3)
P 		74
C 49.3
(1)
M 		30.6
(-2)
A 		24.1
(-3)
A 		61.6
(3)
M 		38
avr 43.1
(-0.4) 		23
(-2.5) 		18.1
(-3) 		75.2
(3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 32.9
(-2.5)
A 		53.3
(0)
M 		29.3
(-3)
P 		50.2
(-0.3)
P 		49
(-0.5)
P 		76.6
(3)
P 		47
B 26.2
(-3)
P 		36.8
(-1.2)
P 		62.1
(3)
P 		28.4
(-2.6)
A 		56.7
(2.1)
P 		42
(-0.4)
P 		36
C 32.2
(-0.6)
A 		52.1
(1.4)
A 		58.3
(2)
P 		7.8
(-3)
A 		49.1
(1.1)
P 		68
(3)
P 		60
avr 30.4
(-2.7) 		47.4
(0.3) 		49.9
(0.8) 		28.8
(-3) 		51.6
(1.1) 		62.2
(3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 15.5
(3)
P 		3.3
(-3)
A 		5.1
(-2.1)
A 		11.7
(1.1)
P 		12
B 11.9
(0.7)
P 		5.8
(-1.7)
A 		2.4
(-3)
A 		17.9
(3)
P 		16
C 16.3
(3)
M 		8.8
(-2.7)
A 		8.4
(-3)
A 		10.4
(-1.5)
M 		8
avr 14.6
(3) 		6
(-2.5) 		5.3
(-3) 		13.3
(2.2) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 6.1
(-3)
A 		19
(3)
M 		6.4
(-2.9)
P 		12.7
(0.1)
P 		14.8
(1)
P 		18.7
(2.9)
P 		13
B 9.1
(-2.4)
P 		12.3
(-1.1)
P 		15.1
(-0.1)
P 		7.5
(-3)
A 		23
(3)
P 		15.6
(0.1)
P 		16
C 11.9
(-0.1)
A 		15.9
(1)
A 		14.8
(0.7)
P 		1.6
(-3)
A 		15.9
(1)
P 		22.8
(3)
P 		21
avr 9
(-2.1) 		15.7
(1.3) 		12.1
(-0.5) 		7.3
(-3) 		17.9
(2.4) 		19
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172401_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8632
Sequence Type Exemplar sequence
Representative Public ID F08B4.3 NCBI
Target Description F08B4.3 /REP_DB=WormBase Gene ID /WP=CE07044 /TR=Q19193 /GB=AAB37726.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069084(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069084 NCBI Caenorhabditis elegans F08B4.3 (F08B4.3) mRNA, complete cds. 11/11 None
SNAP00000041282 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8666234:8666441:1 11/11 None
F08B4.3 ENSEMBL cdna:known chromosome:CEL140:IV:8666234:8666441:1 gene:F08B4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8666239-8666447(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08B4.3
Entrez Gene 184171 Entrez gene
SwissProt Q19193 EMBL-EBI
WormBase CE07044 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37726 Hypothetical protein F08B4.3 [Caenorhabditis elegans] ref|NP_501485.1| F08B4.3 [Caenorhabditis elegans] 1.0E-23
blast CAB16484 Hypothetical protein Y53C12A.1 [Caenorhabditis elegans] ref|NP_496095.1| WEE homolog family member (wee-1.3) [Caenorhabditis elegans] sp|O18209|PMYT1_CAEEL Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase (Myt1 kinase) (Lethal protein 37) 3.0E-9
Sequence
Target Sequence 
>C. ELEGANS:172401_X_AT
acttttggtcactccacaagccaaatgacgtaaaatcagcggaagaccacacaaaatatt
ctactccagaagttctgaacggccgtcccacgaagtcatccgacattttcagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTTTGGTCACTCCACAAGCCAAA 399 101 26 Antisense
CACTCCACAAGCCAAATGACGTAAA 648 197 35 Antisense
TCCACAAGCCAAATGACGTAAAATC 477 587 38 Antisense
GACGTAAAATCAGCGGAAGACCACA 28 377 52 Antisense
AAATCAGCGGAAGACCACACAAAAT 377 117 58 Antisense
GACCACACAAAATATTCTACTCCAG 210 383 70 Antisense
AATATTCTACTCCAGAAGTTCTGAA 3 175 80 Antisense
TACTCCAGAAGTTCTGAACGGCCGT 273 643 87 Antisense
CTCCAGAAGTTCTGAACGGCCGTCC 709 231 89 Antisense
TCCCACGAAGTCATCCGACATTTTC 265 597 111 Antisense
CACGAAGTCATCCGACATTTTCAGT 694 197 114 Antisense
Sequence Source Affymetrix Proprietary Database