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[172400_x_at]

Description F22A3.6 /REP_DB=WormBase Gene ID /WP=CE04442 /TR=Q19698 /GB=AAA83197.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 42      6grp_highest_nonorm > 25      6grp_total > 42      4grp_total > 59      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10223
1 2 3 4 range
A 5737.9
(0.9)
P 		8382.6
(2.8)
P 		8741.5
(3)
P 		66.4
(-3)
A 		8675
B 5914.4
(0.4)
P 		6862.7
(1)
P 		10267.1
(3)
P 		44.4
(-3)
A 		10223
C 5816.5
(0.9)
P 		6713.5
(1.6)
P 		8811.7
(3)
P 		95.7
(-3)
A 		8716
avr 5822.9
(0.8) 		7319.6
(1.7) 		9273.4
(3) 		68.8
(-3) 		9205

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4309
1 2 3 4 5 6 range
A 3023.8
(-1.7)
P 		5306.9
(2.3)
P 		5672.4
(3)
P 		3482.8
(-0.9)
P 		2734.8
(-2.2)
P 		2308.6
(-3)
P 		3364
B 2656.4
(-1.8)
P 		4273.3
(0.7)
P 		5832.9
(3)
P 		2825.8
(-1.5)
P 		2766.5
(-1.6)
P 		1827.7
(-3)
P 		4005
C 2859.7
(-2.1)
P 		4136
(-0.1)
P 		6136.9
(3)
P 		3009.5
(-1.9)
P 		2869.8
(-2.1)
P 		2294.6
(-3)
P 		3842
avr 2846.6
(-1.9) 		4572.1
(0.9) 		5880.7
(3) 		3106
(-1.5) 		2790.4
(-2) 		2143.6
(-3) 		3737
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3062
1 2 3 4 range
A 2089.2
(2.5)
P 		1819.6
(1.8)
P 		2285.2
(3)
P 		9.7
(-3)
A 		2276
B 1886.1
(1.8)
P 		1694.9
(1.3)
P 		2379.1
(3)
P 		9.5
(-3)
A 		2370
C 1925.4
(0.7)
P 		1930.2
(0.8)
P 		3071.1
(3)
P 		16.1
(-3)
A 		3055
avr 1966.9
(1.6) 		1814.9
(1.2) 		2578.5
(3) 		11.8
(-3) 		2567

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1330
1 2 3 4 5 6 range
A 557.4
(-3)
P 		1887.4
(3)
P 		1235.3
(0.1)
P 		883.3
(-1.5)
P 		825.2
(-1.8)
P 		562.1
(-3)
P 		1330
B 924.3
(-1)
P 		1422.4
(3)
P 		1416.4
(3)
P 		748.4
(-2.5)
P 		1124
(0.6)
P 		681.5
(-3)
P 		741
C 1060
(-0.1)
P 		1262
(1.1)
P 		1561.9
(3)
P 		605.2
(-3)
P 		928.6
(-1)
P 		768.1
(-2)
P 		957
avr 847.2
(-1.8) 		1523.9
(3) 		1404.5
(2.2) 		745.6
(-2.5) 		959.3
(-1) 		670.6
(-3) 		853
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172400_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16727
Sequence Type Exemplar sequence
Representative Public ID F22A3.6 NCBI
Target Description F22A3.6 /REP_DB=WormBase Gene ID /WP=CE04442 /TR=Q19698 /GB=AAA83197.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029423 NCBI Caenorhabditis elegans F22A3.6a (F22A3.6) mRNA, complete cds. 11/11 None
GENEFINDER00000004717 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6532599:6536066:-1 10/11 None
F22A3.6a ENSEMBL cdna:known chromosome:CEL140:X:6533504:6536732:-1 gene:F22A3.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029424 3/11 Cross Hyb Matching Probes None
SNAP00000004714 4/11 Cross Hyb Matching Probes None
F22A3.6b 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180928 2 1 NM_001029423 172400_x_at (11), 173051_s_at (11), 194255_x_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6533503-6536732(-) 100.0 100.0
Public Domain and Genome References
Ensembl F22A3.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024594 F22A3.6a [Caenorhabditis elegans] gb|AAA83197.1| Hypothetical protein F22A3.6a [Caenorhabditis elegans] 4.0E-84
blast CAE65750 Hypothetical protein CBG10836 [Caenorhabditis briggsae] 2.0E-77
blast NP_001024594 F22A3.6a [Caenorhabditis elegans] gb|AAA83197.1| Hypothetical protein F22A3.6a [Caenorhabditis elegans] 2.0E-74
blast NP_500206 C45G7.3 [Caenorhabditis elegans] gb|AAC19179.1| Hypothetical protein C45G7.3 [Caenorhabditis elegans] 4.0E-72
blast CAE65750 Hypothetical protein CBG10836 [Caenorhabditis briggsae] 1.0E-67
blast NP_500206 C45G7.3 [Caenorhabditis elegans] gb|AAC19179.1| Hypothetical protein C45G7.3 [Caenorhabditis elegans] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR008597 EMBL-EBI Destabilase 1.2E-71
Pfam IPR008597 EMBL-EBI Destabilase 2.6E-69
Sequence
Target Sequence 
>C. ELEGANS:172400_X_AT
ctgtcgctattgcgtacgtttccgccgactgcctccactgcatctgcatgagagagtcag
gatgcaaaccaatcggttgccacatggacgttggatccctctcctgcggatactatcaga
tcaagattggatactatgaagactgcggacaaccaacaaagaaggctggagagactactg
aagctgcctggaagagatgtgctgatgatcttaactgcgctaccacttgcgtcgagaact
actacaaccgctacaagtctcagtgcaatggacttggaatgggagcttgccagatcatgt
cccgtaaccacaacggaggaccacgtggatgccacaacgccaacaccttggcttact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTCGCTATTGCGTACGTTTCCGC 405 239 41 Antisense
TGCCTCCACTGCATCTGCATGAGAG 338 585 70 Antisense
GTTGCCACATGGACGTTGGATCCCT 535 439 116 Antisense
CCTCTCCTGCGGATACTATCAGATC 562 267 138 Antisense
GAGAGACTACTGAAGCTGCCTGGAA 316 395 209 Antisense
GTGCTGATGATCTTAACTGCGCTAC 161 479 239 Antisense
CGTCGAGAACTACTACAACCGCTAC 383 243 270 Antisense
CAACCGCTACAAGTCTCAGTGCAAT 538 187 285 Antisense
TGGGAGCTTGCCAGATCATGTCCCG 175 555 320 Antisense
CAACGGAGGACCACGTGGATGCCAC 198 187 351 Antisense
CACAACGCCAACACCTTGGCTTACT 204 195 373 Antisense
Sequence Source Affymetrix Proprietary Database