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[172396_at]

Description F27C1.9 /REP_DB=WormBase Gene ID /WP=CE09721 /TR=P91286 /GB=AAB37657.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 21      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 43.4
(-0.9)
M 		37.5
(-1.2)
A 		5.7
(-3)
A 		114.7
(3)
P 		109
B 46.1
(3)
P 		28.2
(0.4)
A 		4.9
(-3)
A 		25.5
(0)
A 		41
C 52.9
(3)
P 		41
(1.2)
A 		12.4
(-3)
A 		39.5
(1)
P 		41
avr 47.5
(1.6) 		35.6
(0.2) 		7.7
(-3) 		59.9
(3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 7.1
(-3)
A 		49.8
(2.7)
A 		51.8
(3)
A 		30.4
(0.1)
A 		18.9
(-1.4)
A 		12.8
(-2.2)
A 		45
B 30.8
(-0.5)
A 		32.9
(-0.1)
P 		49.9
(3)
A 		37.1
(0.7)
A 		17
(-3)
A 		20.9
(-2.3)
P 		33
C 31.3
(2.3)
P 		31.6
(2.3)
A 		2.3
(-3)
A 		4.2
(-2.7)
A 		35.4
(3)
A 		28.2
(1.7)
P 		33
avr 23.1
(-2.1) 		38.1
(3) 		34.7
(1.8) 		23.9
(-1.9) 		23.8
(-1.9) 		20.6
(-3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 15.8
(2.6)
M 		8.1
(-0.4)
A 		1.5
(-3)
A 		16.8
(3)
P 		15
B 14.7
(3)
P 		7
(-0.4)
A 		1.1
(-3)
A 		5.4
(-1.1)
A 		14
C 17.5
(3)
P 		11.8
(0.4)
A 		4.3
(-3)
A 		6.7
(-1.9)
P 		13
avr 16
(3) 		9
(-0.1) 		2.3
(-3) 		9.6
(0.2) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 1.3
(-3)
A 		17.7
(3)
A 		11.3
(0.7)
A 		7.7
(-0.7)
A 		5.7
(-1.4)
A 		3.1
(-2.3)
A 		16
B 10.7
(1.4)
A 		10.9
(1.6)
P 		12.1
(3)
A 		9.8
(0.3)
A 		6.9
(-3)
A 		7.8
(-2)
P 		5
C 11.6
(3)
P 		9.6
(1.9)
A 		0.6
(-3)
A 		0.8
(-2.9)
A 		11.4
(2.9)
A 		9.5
(1.9)
P 		11
avr 7.9
(-1.4) 		12.7
(3) 		8
(-1.3) 		6.1
(-3) 		8
(-1.3) 		6.8
(-2.4) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172396_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1740
Sequence Type Exemplar sequence
Representative Public ID F27C1.9 NCBI
Target Description F27C1.9 /REP_DB=WormBase Gene ID /WP=CE09721 /TR=P91286 /GB=AAB37657.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059197(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059197 NCBI Caenorhabditis elegans F27C1.10 (F27C1.10) mRNA, complete cds. 11/11 A 
SNAP00000014506 ENSEMBL cdna:SNAP chromosome:CEL140:I:5434094:5442892:-1 9/11 None
GENEFINDER00000014525 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5434094:5434424:-1 9/11 None
F27C1.10 ENSEMBL cdna:known chromosome:CEL140:I:5433575:5436785:-1 gene:F27C1.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5433574-5434421(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F27C1.10
Entrez Gene 185008 Entrez gene
SwissProt P91288 EMBL-EBI
WormBase CE30521 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37660 Hypothetical protein F27C1.11 [Caenorhabditis elegans] ref|NP_491599.2| F27C1.11 [Caenorhabditis elegans] 0.0
blast CAE67066 Hypothetical protein CBG12474 [Caenorhabditis briggsae] 0.0
blast AAB37659 Hypothetical protein F27C1.10 [Caenorhabditis elegans] ref|NP_491598.2| F27C1.10 [Caenorhabditis elegans] 1.0E-178
blast AAB37659 Hypothetical protein F27C1.10 [Caenorhabditis elegans] ref|NP_491598.2| F27C1.10 [Caenorhabditis elegans] 1.0E-133
blast CAE67066 Hypothetical protein CBG12474 [Caenorhabditis briggsae] 2.0E-48
blast AAB37659 Hypothetical protein F27C1.10 [Caenorhabditis elegans] ref|NP_491598.2| F27C1.10 [Caenorhabditis elegans] 1.0E-34
blast CAE67066 Hypothetical protein CBG12474 [Caenorhabditis briggsae] 1.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR003533 EMBL-EBI Doublecortin 1.9E-5
Pfam IPR001024 EMBL-EBI Lipoxygenase, LH2 domain 8.8E-21
Pfam IPR000048 EMBL-EBI IQ calmodulin-binding region 2.8E-5
Sequence
Target Sequence 
>C. ELEGANS:172396_AT
ggagctatcaatctgaaagagttgcggaacatagaagtatgggccaaaattggagagccc
atgaattggaagggaaatattacaatagttacatcggatgaaaagatttatgagtcagaa
gatttagaattatcgaaaaacgggcaaatcgcattatccccattgatatttgttggagtg
aatgagccggttattgatgaaaggatggcacaacttcatctcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCTATCAATCTGAAAGAGTTGC 408 521 31 Antisense
GTTGCGGAACATAGAAGTATGGGCC 276 437 51 Antisense
GCCAAAATTGGAGAGCCCATGAATT 102 269 73 Antisense
GGAGAGCCCATGAATTGGAAGGGAA 250 519 82 Antisense
ATATTACAATAGTTACATCGGATGA 478 19 107 Antisense
AACGGGCAAATCGCATTATCCCCAT 242 143 169 Antisense
AAATCGCATTATCCCCATTGATATT 84 119 176 Antisense
ATCCCCATTGATATTTGTTGGAGTG 196 39 186 Antisense
GTTGGAGTGAATGAGCCGGTTATTG 562 415 202 Antisense
GAGCCGGTTATTGATGAAAGGATGG 583 385 214 Antisense
AAGGATGGCACAACTTCATCTCCTT 270 161 231 Antisense
Sequence Source Affymetrix Proprietary Database