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[172388_x_at]

Description B0213.2 /REP_DB=WormBase Gene ID /WP=CE16773 /TR=O44666 /GB=AAC47930.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 37      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 30      6grp_total > 27      4grp_total > 03      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 492
1 2 3 4 range
A 356.7
(-2.5)
P 		464.3
(-0.9)
P 		321.7
(-3)
P 		723.5
(3)
P 		402
B 507.7
(-1.5)
P 		406.7
(-3)
P 		442.5
(-2.5)
P 		813.5
(3)
P 		407
C 412.9
(-1.9)
P 		355.9
(-2.9)
P 		350.2
(-3)
P 		699.6
(3)
P 		349
avr 425.8
(-2.1) 		409
(-2.4) 		371.5
(-3) 		745.5
(3) 		374

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1417
1 2 3 4 5 6 range
A 1013.9
(3)
P 		782.8
(1.1)
P 		735.8
(0.7)
P 		551.9
(-0.9)
P 		295.2
(-3)
P 		747.2
(0.8)
P 		719
B 964.3
(3)
P 		703
(0.5)
P 		493.1
(-1.5)
P 		336
(-3)
P 		550.6
(-1)
P 		790.3
(1.3)
P 		628
C 1712.3
(3)
P 		704.7
(-1.5)
P 		556.2
(-2.2)
P 		381.7
(-3)
P 		468.2
(-2.6)
P 		846.1
(-0.9)
P 		1331
avr 1230.2
(3) 		730.2
(-0.7) 		595
(-1.7) 		423.2
(-3) 		438
(-2.9) 		794.5
(-0.2) 		807
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 129.9
(3)
P 		100.8
(-0.8)
P 		84.1
(-3)
P 		105.9
(-0.1)
P 		46
B 161.9
(2.1)
P 		100.4
(-3)
P 		102.5
(-2.8)
P 		173.4
(3)
P 		73
C 136.7
(3)
P 		102.3
(-3)
P 		122.1
(0.5)
P 		118
(-0.3)
P 		34
avr 142.8
(3) 		101.2
(-3) 		102.9
(-2.8) 		132.4
(1.5) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 558
1 2 3 4 5 6 range
A 186.9
(0.1)
P 		278.4
(3)
P 		160.2
(-0.7)
P 		140
(-1.4)
P 		89.1
(-3)
P 		181.9
(-0.1)
P 		189
B 335.5
(3)
P 		234
(0.5)
P 		119.7
(-2.3)
P 		89
(-3)
P 		223.7
(0.3)
P 		294.7
(2)
P 		247
C 634.7
(3)
P 		215
(-1.5)
P 		141.6
(-2.3)
P 		76.8
(-3)
P 		151.5
(-2.2)
P 		283.2
(-0.8)
P 		558
avr 385.7
(3) 		242.5
(-0) 		140.5
(-2.2) 		101.9
(-3) 		154.8
(-1.9) 		253.3
(0.2) 		284
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172388_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15952
Sequence Type Exemplar sequence
Representative Public ID B0213.2 NCBI
Target Description B0213.2 /REP_DB=WormBase Gene ID /WP=CE16773 /TR=O44666 /GB=AAC47930.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071710(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071710 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-27) (nlp-27) mRNA, complete cds. 11/11 None
SNAP00000025863 ENSEMBL cdna:SNAP chromosome:CEL140:V:3989231:3989526:1 11/11 None
GENEFINDER00000025865 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3989231:3989792:1 11/11 None
B0213.2 ENSEMBL cdna:known chromosome:CEL140:V:3989208:3989584:1 gene:B0213.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3989230-3989526(+) 82.65 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-27
Ensembl B0213.2
Entrez Gene 178806 Entrez gene
SwissProt O44666 EMBL-EBI
WormBase CE16773 Wormbase
Functional Annotations
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504111.1 2 7-26,30-49
B0213.2 2 7-26,30-49
SNAP00000025863 2 7-26,30-49
GENEFINDER00000025865 2 7-26,30-49
Sequence
Target Sequence 
>C. ELEGANS:172388_X_AT
ccagcgcccagtggggatacggtggaatgccatatggtggatacggtggaatgggaggct
acggaatgggaggatacggaatgggatacagacggcgcatgtggggaagcccatacggtg
gatatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAGCGCCCAGTGGGGATACGGTGG 312 269 71 Antisense
GCCCAGTGGGGATACGGTGGAATGC 22 283 76 Antisense
CGGTGGAATGCCATATGGTGGATAC 171 243 90 Antisense
GGTGGATACGGTGGAATGGGAGGCT 644 499 106 Antisense
GGTGGAATGGGAGGCTACGGAATGG 230 497 115 Antisense
GGGAGGCTACGGAATGGGAGGATAC 20 495 123 Antisense
TACGGAATGGGATACAGACGGCGCA 365 647 145 Antisense
GGGATACAGACGGCGCATGTGGGGA 241 497 153 Antisense
AGACGGCGCATGTGGGGAAGCCCAT 293 71 160 Antisense
GGCGCATGTGGGGAAGCCCATACGG 156 537 164 Antisense
TGGGGAAGCCCATACGGTGGATATG 284 497 172 Antisense
Sequence Source Affymetrix Proprietary Database