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[172375_x_at]

Description B0213.4 /REP_DB=WormBase Gene ID /WP=CE16775 /TR=O44664 /GB=AAC47929.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_highest_8_8 > 13      4grp_high_8_8 > 23      6grp_highest_nonorm > 01      6grp_total > 07      4grp_total > 24      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 227
1 2 3 4 range
A 946.6
(-3)
P 		1079.1
(3)
P 		973.3
(-1.8)
P 		967.5
(-2.1)
P 		133
B 950.5
(-1.2)
P 		909.8
(-3)
P 		1045.7
(3)
P 		1041.7
(2.8)
P 		136
C 992.1
(-1.4)
P 		939.7
(-3)
P 		1039.1
(0)
P 		1137
(3)
P 		197
avr 963.1
(-3) 		976.2
(-2.1) 		1019.4
(0.9) 		1048.7
(3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3146
1 2 3 4 5 6 range
A 2961.9
(3)
P 		1976.8
(0.3)
P 		1870
(0)
P 		1223.5
(-1.8)
P 		767.7
(-3)
P 		944.5
(-2.5)
P 		2194
B 3243.5
(3)
P 		1816.1
(-0.7)
P 		1652.7
(-1.1)
P 		924
(-3)
P 		939.3
(-2.9)
P 		909.9
(-3)
P 		2334
C 3913.8
(3)
P 		1765.8
(-1.3)
P 		1343.8
(-2.2)
P 		981.1
(-2.9)
P 		948.5
(-3)
P 		964.1
(-3)
P 		2965
avr 3373.1
(3) 		1852.9
(-0.7) 		1622.2
(-1.2) 		1042.9
(-2.6) 		885.2
(-3) 		939.5
(-2.9) 		2488
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 221
1 2 3 4 range
A 344.7
(3)
P 		234.2
(-0.3)
P 		254.4
(0.3)
P 		141.6
(-3)
P 		203
B 303.1
(3)
P 		224.7
(-2.8)
P 		242.3
(-1.5)
P 		222
(-3)
P 		81
C 328.4
(1.8)
P 		270.2
(-0.2)
P 		362.2
(3)
P 		191.7
(-3)
P 		171
avr 325.4
(3) 		243
(-0.5) 		286.3
(1.3) 		185.1
(-3) 		140

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1253
1 2 3 4 5 6 range
A 546
(1)
P 		703
(3)
P 		407.2
(-0.8)
P 		310.3
(-2)
P 		231.6
(-3)
P 		229.9
(-3)
P 		473
B 1128.6
(3)
P 		604.5
(-0.6)
P 		401.3
(-1.9)
P 		244.7
(-3)
P 		381.6
(-2.1)
P 		339.3
(-2.4)
P 		884
C 1450.7
(3)
P 		538.8
(-1.4)
P 		342
(-2.3)
P 		197.3
(-3)
P 		306.9
(-2.5)
P 		322.7
(-2.4)
P 		1253
avr 1041.8
(3) 		615.4
(-0.2) 		383.5
(-2) 		250.8
(-3) 		306.7
(-2.6) 		297.3
(-2.6) 		791
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172375_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15726
Sequence Type Exemplar sequence
Representative Public ID B0213.4 NCBI
Target Description B0213.4 /REP_DB=WormBase Gene ID /WP=CE16775 /TR=O44664 /GB=AAC47929.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071708(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071708 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-29) (nlp-29) mRNA, complete cds. 11/11 None
SNAP00000025861 ENSEMBL cdna:SNAP chromosome:CEL140:V:3984260:3984550:1 11/11 None
GENEFINDER00000025873 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3984260:3984550:1 11/11 None
B0213.4 ENSEMBL cdna:known chromosome:CEL140:V:3984242:3984662:1 gene:B0213.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071709 2/11 Cross Hyb Matching Probes A 
NM_071707 1/11 Cross Hyb Matching Probes None
SNAP00000025862 2/11 Cross Hyb Matching Probes None
GENEFINDER00000025875 2/11 Cross Hyb Matching Probes None
B0213.5 1/11 Cross Hyb Matching Probes A 
B0213.3 2/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3984259-3984550(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-29
Ensembl B0213.4
Entrez Gene 178805 Entrez gene
SwissProt O44664 EMBL-EBI
WormBase CE16775 Wormbase
Functional Annotations
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504109.1 1 7-29
B0213.4 1 7-29
SNAP00000025861 1 7-29
GENEFINDER00000025873 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:172375_X_AT
atgatttcaacctcttcaattcttgttcttgtcgtccttctcgcctgcttcatggctgcc
agcgcacaatggggatatggaggatatggaagaggatatggaggatatggtggatacgga
cgaggaatgtatggaggctatggacgcggaatgtatggtggatatggacgtggaatgtac
ggaggatacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATTTCAACCTCTTCAATTCTTG 234 47 13 Antisense
CAACCTCTTCAATTCTTGTTCTTGT 396 187 20 Antisense
TCTTCAATTCTTGTTCTTGTCGTCC 116 617 25 Antisense
CTTCATGGCTGCCAGCGCACAATGG 619 221 60 Antisense
GCTGCCAGCGCACAATGGGGATATG 137 297 67 Antisense
GAGGATATGGTGGATACGGACGAGG 415 403 113 Antisense
ACGGACGAGGAATGTATGGAGGCTA 175 91 128 Antisense
TGGAGGCTATGGACGCGGAATGTAT 26 551 144 Antisense
ATGGACGCGGAATGTATGGTGGATA 113 53 152 Antisense
GTATGGTGGATATGGACGTGGAATG 470 455 165 Antisense
GGACGTGGAATGTACGGAGGATACG 261 523 178 Antisense
Sequence Source Affymetrix Proprietary Database