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[172371_x_at]

Description F44E7.3 /REP_DB=WormBase Gene ID /WP=CE10400 /TR=O16248 /GB=AAC25788.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 16      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 18.3
(-3)
A 		80.8
(3)
A 		61.4
(1.1)
A 		53.5
(0.4)
P 		63
B 64
(-1.9)
A 		55
(-3)
A 		85
(0.5)
A 		106.1
(3)
P 		51
C 41.9
(-3)
A 		48
(-2.6)
A 		46.9
(-2.7)
A 		128.8
(3)
P 		87
avr 41.4
(-3) 		61.3
(-0.8) 		64.4
(-0.5) 		96.1
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 12.9
(-3)
A 		58.6
(-0.6)
A 		72.3
(0.1)
P 		97
(1.3)
A 		129
(3)
P 		122.1
(2.6)
P 		116
B 37.2
(-3)
A 		45.5
(-2.5)
A 		62.5
(-1.4)
P 		90.9
(0.5)
A 		127.3
(2.8)
P 		130.5
(3)
P 		93
C 65.2
(-1.6)
A 		39.7
(-3)
A 		79.9
(-0.7)
A 		111.4
(1)
P 		146.6
(3)
P 		129.1
(2)
P 		107
avr 38.4
(-3) 		47.9
(-2.4) 		71.6
(-0.9) 		99.8
(0.8) 		134.3
(3) 		127.2
(2.6) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 6.7
(-3)
A 		17.5
(3)
A 		16
(2.2)
A 		7.8
(-2.4)
P 		11
B 20.4
(1.5)
A 		13.6
(-3)
A 		19.7
(1.1)
A 		22.6
(3)
P 		9
C 13.9
(-2.9)
A 		13.8
(-3)
A 		16.3
(-1.1)
A 		21.7
(3)
P 		8
avr 13.7
(-3) 		15
(-0.9) 		17.3
(2.8) 		17.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 2.4
(-3)
A 		20.8
(0)
A 		15.8
(-0.8)
P 		24.6
(0.6)
A 		38.9
(3)
P 		29.7
(1.5)
P 		37
B 12.9
(-3)
A 		15.2
(-2.6)
A 		15.2
(-2.6)
P 		24.1
(-1.3)
A 		51.7
(3)
P 		48.7
(2.5)
P 		39
C 24.2
(-0.9)
A 		12.1
(-3)
A 		20.3
(-1.6)
A 		22.4
(-1.3)
P 		47.5
(3)
P 		43.2
(2.3)
P 		35
avr 13.2
(-3) 		16
(-2.5) 		17.1
(-2.3) 		23.7
(-1.1) 		46
(3) 		40.5
(2) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172371_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11543
Sequence Type Exemplar sequence
Representative Public ID F44E7.3 NCBI
Target Description F44E7.3 /REP_DB=WormBase Gene ID /WP=CE10400 /TR=O16248 /GB=AAC25788.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072112(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072112 NCBI Caenorhabditis elegans F44E7.3 (F44E7.3) mRNA, complete cds. 11/11 None
SNAP00000006987 ENSEMBL cdna:SNAP chromosome:CEL140:V:5774799:5775051:1 11/11 None
GENEFINDER00000006997 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5774799:5775051:1 11/11 None
F44E7.3 ENSEMBL cdna:known chromosome:CEL140:V:5774799:5775051:1 gene:F44E7.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_072276 1/11 Cross Hyb Matching Probes A 
SNAP00000018290 1/11 Cross Hyb Matching Probes None
GENEFINDER00000018295 1/11 Cross Hyb Matching Probes None
ZK1055.1 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5774798-5775051(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44E7.3
Entrez Gene 185737 Entrez gene
SwissProt O16248 EMBL-EBI
WormBase CE10400 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25788 Hypothetical protein F44E7.3 [Caenorhabditis elegans] ref|NP_504513.1| F44E7.3 [Caenorhabditis elegans] 1.0E-29
Sequence
Target Sequence 
>C. ELEGANS:172371_X_AT
ttgcagttgcaagaattgatgagacaatctgatgaaaagatcgaagttttggagcttcaa
tctcgactcgaattggctcaacaagcttcggatctcactgatcgtcttcatgaagacttg
agagcttcggatgccagggttcaagaaacgttcaagtttctgctcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCAGTTGCAAGAATTGATGAGAC 337 695 19 Antisense
AGTTTTGGAGCTTCAATCTCGACTC 515 63 63 Antisense
GAGCTTCAATCTCGACTCGAATTGG 674 385 70 Antisense
ATCTCGACTCGAATTGGCTCAACAA 329 33 78 Antisense
TGGCTCAACAAGCTTCGGATCTCAC 664 543 92 Antisense
CTCAACAAGCTTCGGATCTCACTGA 340 227 95 Antisense
GATCTCACTGATCGTCTTCATGAAG 632 419 109 Antisense
AGACTTGAGAGCTTCGGATGCCAGG 321 71 132 Antisense
TGAGAGCTTCGGATGCCAGGGTTCA 312 569 137 Antisense
GGATGCCAGGGTTCAAGAAACGTTC 221 511 147 Antisense
AGAAACGTTCAAGTTTCTGCTCTTC 380 73 162 Antisense
Sequence Source Affymetrix Proprietary Database