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[172346_x_at]

Description R12E2.7 /REP_DB=WormBase Gene ID /WP=CE18139 /TR=O61784 /GB=AAC17028.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 43      6grp_highest_nonorm > 32      6grp_total > 20      4grp_total > 49      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3601
1 2 3 4 range
A 14.6
(-3)
A 		3273.8
(2.4)
P 		3605.8
(3)
P 		6.7
(-3)
A 		3599
B 4.7
(-3)
A 		3174.6
(2.6)
P 		3417.4
(3)
P 		17.2
(-3)
A 		3413
C 7.3
(-3)
A 		3281.9
(3)
P 		3077.5
(2.6)
P 		16.3
(-3)
A 		3275
avr 8.9
(-3) 		3243.4
(2.8) 		3366.9
(3) 		13.4
(-3) 		3358

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1246
1 2 3 4 5 6 range
A 1009.7
(-1.9)
P 		1563
(3)
P 		1548.2
(2.9)
P 		979.9
(-2.2)
P 		1149.6
(-0.7)
P 		889.9
(-3)
P 		673
B 953.8
(-3)
P 		1970.8
(3)
P 		1692.2
(1.4)
P 		1269.9
(-1.1)
P 		1205
(-1.5)
P 		1067.1
(-2.3)
P 		1017
C 755.3
(-3)
P 		2001.6
(3)
P 		1253.4
(-0.6)
P 		1548.8
(0.8)
P 		885.4
(-2.4)
P 		877.5
(-2.4)
P 		1246
avr 906.3
(-3) 		1845.1
(3) 		1497.9
(0.8) 		1266.2
(-0.7) 		1080
(-1.9) 		944.8
(-2.8) 		939
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1072
1 2 3 4 range
A 5.3
(-3)
A 		710.7
(1.5)
P 		942.6
(3)
P 		1
(-3)
A 		942
B 1.5
(-3)
A 		784.1
(2.9)
P 		791.9
(3)
P 		3.7
(-3)
A 		790
C 2.4
(-3)
A 		943.6
(2.3)
P 		1072.6
(3)
P 		2.7
(-3)
A 		1070
avr 3.1
(-3) 		812.8
(2.2) 		935.7
(3) 		2.5
(-3) 		933

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 470
1 2 3 4 5 6 range
A 186.1
(-3)
P 		555.9
(3)
P 		337.2
(-0.5)
P 		248.5
(-2)
P 		346.9
(-0.4)
P 		216.7
(-2.5)
P 		370
B 331.9
(-3)
P 		656
(3)
P 		410.9
(-1.5)
P 		336.3
(-2.9)
P 		489.6
(-0.1)
P 		397.9
(-1.8)
P 		324
C 279.9
(-3)
P 		610.8
(3)
P 		319
(-2.3)
P 		311.5
(-2.4)
P 		286.5
(-2.9)
P 		293.8
(-2.7)
P 		331
avr 266
(-3) 		607.6
(3) 		355.7
(-1.4) 		298.8
(-2.4) 		374.3
(-1.1) 		302.8
(-2.4) 		342
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172346_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.519
Sequence Type Exemplar sequence
Representative Public ID R12E2.7 NCBI
Target Description R12E2.7 /REP_DB=WormBase Gene ID /WP=CE18139 /TR=O61784 /GB=AAC17028.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058910(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058910 NCBI Caenorhabditis elegans R12E2.7 (R12E2.7) mRNA, complete cds. 11/11 None
SNAP00000012677 ENSEMBL cdna:SNAP chromosome:CEL140:I:4154069:4154405:-1 11/11 None
GENEFINDER00000012695 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4154069:4158699:-1 11/11 None
R12E2.7 ENSEMBL cdna:known chromosome:CEL140:I:4153958:4154439:-1 gene:R12E2.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4154068-4154405(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12E2.7
Entrez Gene 172002 Entrez gene
SwissProt O61784 EMBL-EBI
WormBase CE18139 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491312 R12E2.8 [Caenorhabditis elegans] gb|AAC17029.3| Hypothetical protein R12E2.8 [Caenorhabditis elegans] 1.0E-145
blast CAE66709 Hypothetical protein CBG12054 [Caenorhabditis briggsae] 1.0E-111
blast BAC41519.1 galaxin [Galaxea fascicularis] pir||JC7958 galaxin precursor - reef coral 2.0E-18
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012695 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:172346_X_AT
tgcttctgcttttgtcgcagcacttgttgcatctggaaacgcctgcctcggaggatccgg
aggtggtggatgctgcccacaggctcagccaagctgcggaaacccatgcggaggaggtct
tggtccagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTTCTGCTTTTGTCGCAGCACTT 701 579 27 Antisense
CTTTTGTCGCAGCACTTGTTGCATC 470 221 35 Antisense
GTCGCAGCACTTGTTGCATCTGGAA 365 475 40 Antisense
GCAGCACTTGTTGCATCTGGAAACG 396 313 43 Antisense
CTTGTTGCATCTGGAAACGCCTGCC 218 219 49 Antisense
CCTGCCTCGGAGGATCCGGAGGTGG 128 267 68 Antisense
TCCGGAGGTGGTGGATGCTGCCCAC 147 595 82 Antisense
CCACAGGCTCAGCCAAGCTGCGGAA 97 275 103 Antisense
CAGGCTCAGCCAAGCTGCGGAAACC 272 209 106 Antisense
TGCGGAAACCCATGCGGAGGAGGTC 621 551 121 Antisense
ATGCGGAGGAGGTCTTGGTCCAGCT 601 41 132 Antisense
Sequence Source Affymetrix Proprietary Database