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[172344_x_at]

Description F09F7.8 /REP_DB=WormBase Gene ID /WP=CE01240 /TR=Q19279 /GB=AAA50700.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      6grp_highest_nonorm > 80      6grp_total > 25      4grp_total > 49      6grp_highest_sub > 01_02      6grp_cgc > 43      6grp_high1_sub > 01_02      6grp_cgc_sub > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 498
1 2 3 4 range
A 7
(-3)
A 		476.3
(3)
P 		445.4
(2.6)
P 		19.7
(-2.8)
A 		469
B 5.3
(-3)
A 		488.4
(3)
P 		481.9
(2.9)
P 		11.9
(-2.9)
A 		483
C 5.1
(-3)
A 		503.5
(3)
P 		444.3
(2.3)
P 		5.3
(-3)
A 		498
avr 5.8
(-3) 		489.4
(3) 		457.2
(2.6) 		12.3
(-2.9) 		484

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 417
1 2 3 4 5 6 range
A 214.6
(-1.7)
P 		447.5
(3)
P 		159.4
(-2.9)
P 		171
(-2.6)
P 		152.8
(-3)
P 		202.6
(-2)
P 		295
B 255.9
(0.6)
P 		356.4
(3)
P 		198.7
(-0.8)
P 		105.8
(-3)
P 		143.3
(-2.1)
P 		114.7
(-2.8)
P 		251
C 523
(3)
P 		309.7
(-0.2)
P 		231.2
(-1.4)
P 		144.9
(-2.7)
P 		237.9
(-1.3)
P 		126.1
(-3)
P 		397
avr 331.2
(2) 		371.2
(3) 		196.4
(-1.5) 		140.6
(-3) 		178
(-2) 		147.8
(-2.8) 		231
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 2.5
(-3)
A 		103.4
(2.3)
P 		116.4
(3)
P 		2.9
(-3)
A 		114
B 1.7
(-3)
A 		120.6
(3)
P 		111.7
(2.6)
P 		2.5
(-3)
A 		119
C 1.7
(-3)
A 		144.8
(2.6)
P 		154.9
(3)
P 		0.9
(-3)
A 		154
avr 2
(-3) 		122.9
(2.8) 		127.7
(3) 		2.1
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 39.6
(-2.8)
P 		159.1
(3)
P 		34.7
(-3)
P 		43.4
(-2.6)
P 		46.1
(-2.5)
P 		49.3
(-2.3)
P 		124
B 89
(1)
P 		118.6
(3)
P 		48.3
(-1.7)
P 		28
(-3)
P 		58.2
(-1)
P 		42.8
(-2)
P 		91
C 193.8
(3)
P 		94.5
(-0.6)
P 		58.8
(-1.9)
P 		29.1
(-3)
P 		77
(-1.3)
P 		42.2
(-2.5)
P 		165
avr 107.5
(1.9) 		124.1
(3) 		47.3
(-2.1) 		33.5
(-3) 		60.4
(-1.2) 		44.8
(-2.3) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172344_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6093
Sequence Type Exemplar sequence
Representative Public ID F09F7.8 NCBI
Target Description F09F7.8 /REP_DB=WormBase Gene ID /WP=CE01240 /TR=Q19279 /GB=AAA50700.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065793(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065793 NCBI Caenorhabditis elegans Nematode Specific Peptide family, group B family member (nspb-12) (nspb-12) mRNA, complete cds. 11/11 None
SNAP00000025401 ENSEMBL cdna:SNAP chromosome:CEL140:III:5568268:5568703:-1 11/11 None
GENEFINDER00000025407 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5568268:5568489:-1 11/11 None
F09F7.8 ENSEMBL cdna:known chromosome:CEL140:III:5568164:5568489:-1 gene:F09F7.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064628 1/11 Cross Hyb Matching Probes A 
NM_064631 1/11 Cross Hyb Matching Probes A 
NM_001026733 1/11 Cross Hyb Matching Probes None
SNAP00000024263 2/11 Cross Hyb Matching Probes None
SNAP00000024265 1/11 Cross Hyb Matching Probes None
SNAP00000024270 1/11 Cross Hyb Matching Probes None
GENEFINDER00000024278 1/11 Cross Hyb Matching Probes None
GENEFINDER00000024282 1/11 Cross Hyb Matching Probes None
GENEFINDER00000024283 1/11 Cross Hyb Matching Probes None
C01G12.2 1/11 Cross Hyb Matching Probes A 
C01G12.11 1/11 Cross Hyb Matching Probes None
C01G12.6 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5568266-5568488(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nspb-12
Ensembl F09F7.8
Entrez Gene 184261 Entrez gene
SwissProt Q19279 EMBL-EBI
WormBase CE38594 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498194 Nematode Specific Peptide family, group B family member (nspb-12) [Caenorhabditis elegans] gb|AAA50700.2| Hypothetical protein F09F7.8 [Caenorhabditis elegans] 6.0E-22
blast CAE64304 Hypothetical protein CBG08980 [Caenorhabditis briggsae] 6.0E-19
blast T16013 hypothetical protein F09F7.8 - Caenorhabditis elegans 4.0E-14
blast NP_498194 Nematode Specific Peptide family, group B family member (nspb-12) [Caenorhabditis elegans] gb|AAA50700.2| Hypothetical protein F09F7.8 [Caenorhabditis elegans] 5.0E-14
blast T16013 hypothetical protein F09F7.8 - Caenorhabditis elegans 5.0E-14
Sequence
Target Sequence 
>C. ELEGANS:172344_X_AT
atgatcgtcggatcccatcaacaatacgttgtcccatcctactcggccgcctatgttcca
tctgcctacgcctacccatcagtttattctccatacgtagccgctgcagcctatccatca
gtctgggcttggggatcaaacaagaataaggatgatgctgctccacgtgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATCGTCGGATCCCATCAACAAT 652 45 13 Antisense
GATCCCATCAACAATACGTTGTCCC 36 417 23 Antisense
ATCAACAATACGTTGTCCCATCCTA 343 27 29 Antisense
CTACCCATCAGTTTATTCTCCATAC 619 225 84 Antisense
ACCCATCAGTTTATTCTCCATACGT 75 89 86 Antisense
TCAGTTTATTCTCCATACGTAGCCG 464 621 91 Antisense
TGCAGCCTATCCATCAGTCTGGGCT 348 577 117 Antisense
CCTATCCATCAGTCTGGGCTTGGGG 586 267 122 Antisense
TCCATCAGTCTGGGCTTGGGGATCA 218 587 126 Antisense
CAGTCTGGGCTTGGGGATCAAACAA 657 207 131 Antisense
TAAGGATGATGCTGCTCCACGTGCA 214 637 159 Antisense
Sequence Source Affymetrix Proprietary Database