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[172333_x_at]

Description F44D12.5 /REP_DB=WormBase Gene ID /WP=CE01192 /TR=Q27317 /GB=CAA92601.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 11      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 285
1 2 3 4 range
A 14.9
(-3)
A 		24.5
(-2.7)
A 		14.6
(-3)
A 		195.5
(3)
A 		181
B 58.6
(3)
A 		43.5
(0.9)
A 		16.1
(-3)
A 		47.7
(1.5)
A 		43
C 17.1
(-2.9)
A 		34.3
(-2.6)
A 		14.6
(-3)
A 		299.6
(3)
A 		285
avr 30.2
(-2.5) 		34.1
(-2.3) 		15.1
(-3) 		180.9
(3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2008
1 2 3 4 5 6 range
A 9.6
(-3)
A 		8.1
(-3)
A 		59.3
(-2.8)
A 		242.8
(-2.2)
A 		1530.8
(2.3)
P 		1741.5
(3)
P 		1733
B 12.6
(-3)
A 		50.3
(-2.9)
A 		18
(-3)
A 		197.7
(-2.3)
A 		1541.7
(3)
P 		1541.8
(3)
P 		1529
C 12.4
(-3)
A 		185.4
(-2.5)
A 		10.6
(-3)
A 		249.8
(-2.3)
A 		1708.7
(2.1)
P 		2016.3
(3)
P 		2006
avr 11.5
(-3) 		81.3
(-2.8) 		29.3
(-2.9) 		230.1
(-2.3) 		1593.7
(2.4) 		1766.5
(3) 		1755
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 5.4
(-2.6)
A 		5.3
(-2.6)
A 		3.8
(-3)
A 		28.6
(3)
A 		25
B 18.7
(3)
A 		10.7
(-0.2)
A 		3.7
(-3)
A 		10.2
(-0.4)
A 		15
C 5.7
(-2.9)
A 		9.9
(-2.4)
A 		5.1
(-3)
A 		50.5
(3)
A 		45
avr 9.9
(-1.7) 		8.6
(-2) 		4.2
(-3) 		29.8
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 673
1 2 3 4 5 6 range
A 1.8
(-3)
A 		2.9
(-3)
A 		12.9
(-2.9)
A 		61.6
(-2.2)
A 		461.9
(3)
P 		424
(2.5)
P 		460
B 4.4
(-3)
A 		16.7
(-2.9)
A 		4.4
(-3)
A 		52.4
(-2.5)
A 		626.4
(3)
P 		574.9
(2.5)
P 		622
C 4.6
(-3)
A 		56.6
(-2.5)
A 		2.7
(-3)
A 		50.2
(-2.6)
A 		552.9
(1.9)
P 		675
(3)
P 		672
avr 3.6
(-3) 		25.4
(-2.8) 		6.7
(-3) 		54.7
(-2.4) 		547.1
(2.9) 		558
(3) 		554
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172333_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8371
Sequence Type Exemplar sequence
Representative Public ID F44D12.5 NCBI
Target Description F44D12.5 /REP_DB=WormBase Gene ID /WP=CE01192 /TR=Q27317 /GB=CAA92601.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069410(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069410 NCBI Caenorhabditis elegans F44D12.5 (F44D12.5) mRNA, complete cds. 11/11 None
SNAP00000020515 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10029055:10029384:1 11/11 None
GENEFINDER00000020527 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10029055:10029384:1 11/11 None
F44D12.5 ENSEMBL cdna:known chromosome:CEL140:IV:10029055:10029384:1 gene:F44D12.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065614 2/11 Cross Hyb Matching Probes A B 
NM_069407 8/11 Cross Hyb Matching Probes A B 
NM_069412 8/11 Cross Hyb Matching Probes A B 
SNAP00000029549 2/11 Cross Hyb Matching Probes None
GENEFINDER00000029561 2/11 Cross Hyb Matching Probes None
SNAP00000020512 8/11 Cross Hyb Matching Probes None
SNAP00000020518 8/11 Cross Hyb Matching Probes None
GENEFINDER00000020520 8/11 Cross Hyb Matching Probes None
GENEFINDER00000020526 8/11 Cross Hyb Matching Probes None
C35D10.11 2/11 Cross Hyb Matching Probes A B 
F44D12.3 8/11 Cross Hyb Matching Probes A B 
F44D12.7 8/11 Cross Hyb Matching Probes A B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10029060-10029390(+) 100.0 100.0
chrIV:10035615-10035945(+) 98.79 98.79
chrIV:10022326-10022656(-) 98.79 98.79
Public Domain and Genome References
Gene Title
Ensembl F44D12.5
Entrez Gene 260109 Entrez gene
SwissProt Q27317 EMBL-EBI
WormBase CE01192 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92603 Hypothetical protein F44D12.7 [Caenorhabditis elegans] emb|CAA92601.1| Hypothetical protein F44D12.5 [Caenorhabditis elegans] emb|CAA92599.1| Hypothetical protein F44D12.3 [Caenorhabditis elegans] ref|NP_501811.1| F44D12.5 [Caenorhabditis elegans] ref|NP_501813.1| F44D12.7 [Caenorhabditis elegans] ref|NP_501808.1| F44D12.3 [Caenorhabditis elegans] ref|NP_498015.1| C35D10.11 [Caenorhabditis elegans] gb|AAA62567.1| Hypothetical protein C35D10.11 [Caenorhabditis elegans] 7.0E-43
blast CAE59021 Hypothetical protein CBG02298 [Caenorhabditis briggsae] 1.0E-40
blast CAE73222 Hypothetical protein CBG20628 [Caenorhabditis briggsae] 6.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.7E-32
Sequence
Target Sequence 
>C. ELEGANS:172333_X_AT
gtgcactgttccagctgctggaggtgcttccacccacaaacttgttaatgctggagctga
gaagatgatcttcaagatcaagtcttcaaacaacaacgaataccgcatcactccggtatt
cggattcgttgatccatctggttcaaaggacatcacaatcactcgtactgctggagcacc
aaaagaagacaagcttgttgttcactttgccgctgctccggctgatgcaactgatgccca
agctgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCACTGTTCCAGCTGCTGGAGGT 109 481 39 Antisense
TGCTTCCACCCACAAACTTGTTAAT 567 577 63 Antisense
CAACAACGAATACCGCATCACTCCG 531 189 129 Antisense
CCGCATCACTCCGGTATTCGGATTC 312 257 141 Antisense
TCCGGTATTCGGATTCGTTGATCCA 290 583 150 Antisense
GATTCGTTGATCCATCTGGTTCAAA 410 433 161 Antisense
AAGGACATCACAATCACTCGTACTG 658 159 184 Antisense
TCACTCGTACTGCTGGAGCACCAAA 312 627 197 Antisense
CAAGCTTGTTGTTCACTTTGCCGCT 693 185 228 Antisense
CTGCTCCGGCTGATGCAACTGATGC 295 237 251 Antisense
TGATGCAACTGATGCCCAAGCTGCG 232 567 261 Antisense
Sequence Source Affymetrix Proprietary Database