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[172327_x_at]

Description F44D12.7 /REP_DB=WormBase Gene ID /WP=CE01192 /TR=Q27317 /GB=CAA92603.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 03      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 275
1 2 3 4 range
A 88.1
(-3)
A 		106.3
(-2.3)
A 		119.8
(-1.8)
A 		248.5
(3)
P 		160
B 102.4
(1.6)
A 		54.3
(-3)
A 		78.7
(-0.7)
A 		117.7
(3)
A 		63
C 117.3
(-1.8)
A 		102
(-2.2)
A 		65.3
(-3)
A 		329.7
(3)
P 		264
avr 102.6
(-2.4) 		87.5
(-3) 		87.9
(-3) 		232
(3) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2248
1 2 3 4 5 6 range
A 84
(-2.9)
A 		47.1
(-3)
A 		129.9
(-2.8)
P 		271.5
(-2.4)
P 		1226.2
(0.3)
P 		2192.9
(3)
P 		2146
B 73.5
(-3)
A 		87.7
(-2.9)
P 		68.2
(-3)
A 		227.7
(-2.5)
P 		1446.5
(1.7)
P 		1818.7
(3)
P 		1751
C 51.8
(-3)
A 		213.9
(-2.5)
P 		35
(-3)
A 		251.4
(-2.4)
P 		1547.9
(1)
P 		2283.1
(3)
P 		2248
avr 69.8
(-3) 		116.2
(-2.9) 		77.7
(-3) 		250.2
(-2.5) 		1406.9
(1) 		2098.2
(3) 		2028
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 32.1
(1.1)
A 		23.1
(-3)
A 		31.3
(0.7)
A 		36.4
(3)
P 		13
B 32.6
(3)
A 		13.4
(-3)
A 		18.2
(-1.5)
A 		25.1
(0.7)
A 		19
C 38.8
(-0.1)
A 		29.3
(-1.8)
A 		22.7
(-3)
A 		55.6
(3)
P 		33
avr 34.5
(1.4) 		21.9
(-3) 		24.1
(-2.2) 		39
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 755
1 2 3 4 5 6 range
A 15.5
(-3)
A 		16.7
(-3)
A 		28.3
(-2.9)
P 		68.9
(-2.4)
P 		370
(1.1)
P 		533.9
(3)
P 		518
B 25.6
(-2.9)
A 		29.2
(-2.9)
P 		16.6
(-3)
A 		60.3
(-2.6)
P 		587.7
(2.2)
P 		678.2
(3)
P 		662
C 19.2
(-2.9)
A 		65.3
(-2.6)
P 		8.9
(-3)
A 		50.5
(-2.7)
P 		500.9
(0.9)
P 		764.3
(3)
P 		755
avr 20.1
(-3) 		37.1
(-2.8) 		17.9
(-3) 		59.9
(-2.6) 		486.2
(1.4) 		658.8
(3) 		641
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172327_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8757
Sequence Type Exemplar sequence
Representative Public ID F44D12.7 NCBI
Target Description F44D12.7 /REP_DB=WormBase Gene ID /WP=CE01192 /TR=Q27317 /GB=CAA92603.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069407(11), NM_069412(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069407 NCBI Caenorhabditis elegans F44D12.3 (F44D12.3) mRNA, complete cds. 11/11 None
NM_069412 NCBI Caenorhabditis elegans F44D12.7 (F44D12.7) mRNA, complete cds. 11/11 None
SNAP00000020512 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10022321:10022650:-1 11/11 None
SNAP00000020518 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10035610:10035939:1 11/11 None
GENEFINDER00000020520 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10035610:10035939:1 11/11 None
GENEFINDER00000020526 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10022321:10022650:-1 11/11 None
F44D12.3 ENSEMBL cdna:known chromosome:CEL140:IV:10022321:10022650:-1 gene:F44D12.3 11/11 None
F44D12.7 ENSEMBL cdna:known chromosome:CEL140:IV:10035610:10035939:1 gene:F44D12.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069410 7/11 Cross Hyb Matching Probes A B 
NM_065614 3/11 Cross Hyb Matching Probes A B 
SNAP00000029549 3/11 Cross Hyb Matching Probes None
GENEFINDER00000029561 3/11 Cross Hyb Matching Probes None
SNAP00000020515 7/11 Cross Hyb Matching Probes None
GENEFINDER00000020527 7/11 Cross Hyb Matching Probes None
C35D10.11 3/11 Cross Hyb Matching Probes A B 
F44D12.5 7/11 Cross Hyb Matching Probes A B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10035615-10035945(+) 100.0 100.0
chrIV:10029060-10029390(+) 98.79 98.79
chrIII:4868376-4868704(-) 90.61 90.61
chrIV:10022326-10022656(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl F44D12.3
F44D12.7
Entrez Gene 177863 Entrez gene
177866 Entrez gene
SwissProt Q27317 EMBL-EBI
WormBase CE01192 Wormbase
CE01192 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92603 Hypothetical protein F44D12.7 [Caenorhabditis elegans] emb|CAA92601.1| Hypothetical protein F44D12.5 [Caenorhabditis elegans] emb|CAA92599.1| Hypothetical protein F44D12.3 [Caenorhabditis elegans] ref|NP_501811.1| F44D12.5 [Caenorhabditis elegans] ref|NP_501813.1| F44D12.7 [Caenorhabditis elegans] ref|NP_501808.1| F44D12.3 [Caenorhabditis elegans] ref|NP_498015.1| C35D10.11 [Caenorhabditis elegans] gb|AAA62567.1| Hypothetical protein C35D10.11 [Caenorhabditis elegans] 7.0E-43
blast CAE59021 Hypothetical protein CBG02298 [Caenorhabditis briggsae] 1.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.7E-32
Sequence
Target Sequence 
>C. ELEGANS:172327_X_AT
ttccagctgctggaggtgcttctacccacaaacttgtcaatgctggagctgagaagatga
tcttcaagatcaagtcttcaaacaacaacgaataccgcatcactccggtctttggattcg
ttgatccatctggttcaaaggacatcacaatcactcgtactgctggagcaccaaaagaag
acaagcttgttgttcactttgccgctgctccggctgatgcaactgatgcccaagctgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCAGCTGCTGGAGGTGCTTCTAC 520 701 47 Antisense
TGCTTCTACCCACAAACTTGTCAAT 569 577 63 Antisense
CAAACTTGTCAATGCTGGAGCTGAG 677 191 75 Antisense
CAACAACGAATACCGCATCACTCCG 532 189 129 Antisense
ATCACTCCGGTCTTTGGATTCGTTG 689 27 145 Antisense
GATTCGTTGATCCATCTGGTTCAAA 411 433 161 Antisense
AAGGACATCACAATCACTCGTACTG 659 159 184 Antisense
TCACTCGTACTGCTGGAGCACCAAA 313 627 197 Antisense
CAAGCTTGTTGTTCACTTTGCCGCT 694 185 228 Antisense
CTGCTCCGGCTGATGCAACTGATGC 296 237 251 Antisense
TGATGCAACTGATGCCCAAGCTGCG 233 567 261 Antisense
Sequence Source Affymetrix Proprietary Database