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[172326_x_at]

Description T20D4.9 /REP_DB=WormBase Gene ID /WP=CE13796 /TR=P91466 /GB=AAB37588.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 26.4
(-1.8)
A 		3.7
(-3)
A 		4.4
(-3)
A 		118.8
(3)
P 		115
B 33.2
(0.9)
A 		15.6
(-1.6)
A 		5.3
(-3)
A 		48
(3)
A 		43
C 32.2
(-2.4)
A 		11.1
(-3)
A 		20.7
(-2.7)
A 		207.6
(3)
P 		197
avr 30.6
(-1.9) 		10.1
(-3) 		10.1
(-3) 		124.8
(3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 13.6
(-3)
A 		17.8
(-2.3)
A 		43.3
(1.8)
A 		14.9
(-2.8)
A 		40.4
(1.3)
A 		50.9
(3)
A 		37
B 36.6
(-0.4)
A 		40.7
(0.5)
A 		37.7
(-0.2)
A 		52.2
(3)
A 		24.8
(-3)
A 		38.9
(0.1)
A 		27
C 17
(-1.6)
A 		56.3
(3)
P 		40.7
(1.2)
A 		35.6
(0.6)
A 		4.9
(-3)
A 		6.5
(-2.8)
A 		51
avr 22.4
(-3) 		38.3
(2.2) 		40.6
(3) 		34.2
(0.9) 		23.4
(-2.7) 		32.1
(0.2) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 9.6
(0.2)
A 		0.8
(-3)
A 		1.2
(-2.9)
A 		17.4
(3)
P 		17
B 10.6
(3)
A 		3.9
(-1.3)
A 		1.2
(-3)
A 		10.2
(2.7)
A 		9
C 10.7
(-1.6)
A 		3.2
(-3)
A 		7.2
(-2.2)
A 		35
(3)
P 		32
avr 10.3
(-0.5) 		2.6
(-3) 		3.2
(-2.8) 		20.9
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 2.5
(-3)
A 		6.3
(-0.7)
A 		9.4
(1.2)
A 		3.8
(-2.2)
A 		12.2
(2.9)
A 		12.4
(3)
A 		10
B 12.7
(1)
A 		13.5
(1.9)
A 		9.2
(-3)
A 		13.8
(2.2)
A 		10.1
(-2)
A 		14.5
(3)
A 		5
C 6.3
(-1.2)
A 		17.2
(3)
P 		10.4
(0.4)
A 		7.1
(-0.9)
A 		1.6
(-3)
A 		2.2
(-2.8)
A 		16
avr 7.2
(-3) 		12.3
(3) 		9.7
(-0.1) 		8.2
(-1.8) 		8
(-2.1) 		9.7
(-0.1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172326_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15041
Sequence Type Exemplar sequence
Representative Public ID T20D4.9 NCBI
Target Description T20D4.9 /REP_DB=WormBase Gene ID /WP=CE13796 /TR=P91466 /GB=AAB37588.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071551(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071551 NCBI Caenorhabditis elegans T20D4.9 (T20D4.9) mRNA, complete cds. 11/11 None
SNAP00000008847 ENSEMBL cdna:SNAP chromosome:CEL140:V:3401354:3405021:1 9/11 None
GENEFINDER00000008863 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3401471:3405021:1 9/11 None
T20D4.9 ENSEMBL cdna:known chromosome:CEL140:V:3402737:3405021:1 gene:T20D4.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071552 1/11 Cross Hyb Matching Probes A 
NM_071497 1/11 Cross Hyb Matching Probes A 
NM_071520 2/11 Cross Hyb Matching Probes A 
SNAP00000008848 1/11 Cross Hyb Matching Probes None
GENEFINDER00000008867 1/11 Cross Hyb Matching Probes None
SNAP00000020303 2/11 Cross Hyb Matching Probes None
GENEFINDER00000020322 2/11 Cross Hyb Matching Probes None
SNAP00000028210 1/11 Cross Hyb Matching Probes None
GENEFINDER00000028228 1/11 Cross Hyb Matching Probes None
T28A11.17 1/11 Cross Hyb Matching Probes A 
C17B7.8 2/11 Cross Hyb Matching Probes A 
T20D4.8 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3402736-3405021(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.9
Entrez Gene 188645 Entrez gene
SwissProt P91466 EMBL-EBI
WormBase CE13796 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37588 Hypothetical protein T20D4.9 [Caenorhabditis elegans] ref|NP_503952.1| T20D4.9 [Caenorhabditis elegans] 0.0
blast AAF59425 Hypothetical protein Y19D10B.6 [Caenorhabditis elegans] ref|NP_503636.1| Y19D10B.6 [Caenorhabditis elegans] 0.0
blast AAB37589 Hypothetical protein T20D4.8 [Caenorhabditis elegans] ref|NP_503953.1| T20D4.8 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 7.0E-85
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 3.1E-88
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 9.3E-36
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 3.1E-88
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 9.3E-36
Sequence
Target Sequence 
>C. ELEGANS:172326_X_AT
gtcagtcatgggtgttaatcctttttgttggctaacgatagccgacaccttttccgtgct
tgcccatgagctcatgcactttgtctatccaagcggaacagagttactgactcctaatgt
tacaaaagcagcaaacaagtgtacacaagatgaagtcaagatgatgggtgactctgatgc
cgtgaaaccaatagatggatggttcagtaaaaaggtcactcatgaagatttagcaaacat
aatgggattaaggatggttatgaagatggcggcaaagaaatcggtgaatgaagagcagct
gaaaaatgcgcttgagacaattttgggcggattatgcatccaaggcaaaccaaaaaactc
aattctaccacatcatcatccctttgaaatatccatcaacactgcagttcgccagtatcc
attgttcagttcactctatgggtgtcgccctggtgatcgaatgtttgctgaagcggaaaa
gttttgcaatccactgggcggcgaggtgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGTCATGGGTGTTAATCCTTTT 545 465 780 Antisense
TAATCCTTTTTGTTGGCTAACGATA 384 633 795 Antisense
GCTCATGCACTTTGTCTATCCAAGC 431 301 849 Antisense
GATGATGGGTGACTCTGATGCCGTG 433 411 939 Antisense
TGCGCTTGAGACAATTTTGGGCGGA 83 583 1086 Antisense
AAACTCAATTCTACCACATCATCAT 54 129 1134 Antisense
AATATCCATCAACACTGCAGTTCGC 635 173 1167 Antisense
TTCGCCAGTATCCATTGTTCAGTTC 697 697 1187 Antisense
TCAGTTCACTCTATGGGTGTCGCCC 180 619 1205 Antisense
GTGTCGCCCTGGTGATCGAATGTTT 305 487 1221 Antisense
CAATCCACTGGGCGGCGAGGTGAAT 709 167 1266 Antisense
Sequence Source Affymetrix Proprietary Database