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[172318_x_at]

Description T22H2.5B /REP_DB=WormBase Gene ID /WP=CE16440 /TR=O45799 /GB=CAB04754.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 83      6grp_total > 39      4grp_total > 38      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 472
1 2 3 4 range
A 491.3
(3)
P 		301.7
(-1.2)
P 		221.9
(-3)
P 		345.8
(-0.2)
P 		269
B 533.9
(3)
P 		294
(-1.1)
P 		183.9
(-3)
P 		329.1
(-0.5)
P 		350
C 655.6
(3)
P 		265.4
(-2.4)
P 		223
(-3)
P 		272.6
(-2.3)
P 		433
avr 560.3
(3) 		287
(-1.7) 		209.6
(-3) 		315.8
(-1.2) 		351

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 489
1 2 3 4 5 6 range
A 631.2
(3)
P 		453
(0)
P 		268.8
(-3)
P 		352.5
(-1.6)
P 		378.8
(-1.2)
P 		502.5
(0.9)
P 		362
B 546.5
(2)
P 		307.3
(-2.4)
P 		275.3
(-3)
P 		400.3
(-0.7)
P 		546.5
(2)
P 		599.1
(3)
P 		324
C 731.6
(3)
P 		295.2
(-2.4)
P 		242.5
(-3)
P 		334.6
(-1.9)
P 		450.6
(-0.4)
P 		458.7
(-0.3)
P 		489
avr 636.4
(3) 		351.8
(-1.6) 		262.2
(-3) 		362.5
(-1.4) 		458.6
(0.1) 		520.1
(1.1) 		374
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 178.9
(3)
P 		65.5
(-2.3)
P 		58
(-2.7)
P 		50.6
(-3)
P 		128
B 170.2
(3)
P 		72.6
(-1.6)
P 		42.6
(-3)
P 		70.1
(-1.7)
P 		128
C 217
(3)
P 		76.3
(-1.9)
P 		77.7
(-1.9)
P 		46
(-3)
P 		171
avr 188.7
(3) 		71.5
(-2.3) 		59.4
(-2.8) 		55.6
(-3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 116.4
(0.4)
P 		161.1
(3)
P 		58.5
(-3)
P 		89.4
(-1.2)
P 		114.3
(0.3)
P 		122.3
(0.7)
P 		103
B 190.2
(1.7)
P 		102.3
(-1.6)
P 		66.9
(-3)
P 		106
(-1.5)
P 		222
(2.9)
P 		223.4
(3)
P 		157
C 271.2
(3)
P 		90.1
(-2.2)
P 		61.7
(-3)
P 		67.3
(-2.8)
P 		145.8
(-0.6)
P 		153.5
(-0.4)
P 		210
avr 192.6
(3) 		117.8
(-0.4) 		62.4
(-3) 		87.6
(-1.8) 		160.7
(1.5) 		166.4
(1.8) 		130
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172318_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1344
Sequence Type Exemplar sequence
Representative Public ID T22H2.5B NCBI
Target Description T22H2.5B /REP_DB=WormBase Gene ID /WP=CE16440 /TR=O45799 /GB=CAB04754.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060576(10), NM_060575(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060576 NCBI Caenorhabditis elegans T22H2.5a (T22H2.5) mRNA, complete cds. 10/11 A 
NM_060575 NCBI Caenorhabditis elegans T22H2.5b (T22H2.5) mRNA, complete cds. 10/11 A 
GENEFINDER00000038048 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11697005:11703607:-1 11/11 None
SNAP00000038040 ENSEMBL cdna:SNAP chromosome:CEL140:I:11697459:11698943:-1 10/11 None
T22H2.5a.2 ENSEMBL cdna:known chromosome:CEL140:I:11696384:11698954:-1 gene:T22H2.5 10/11 None
T22H2.5a.1 ENSEMBL cdna:known chromosome:CEL140:I:11696384:11698952:-1 gene:T22H2.5 10/11 None
T22H2.5b ENSEMBL cdna:known chromosome:CEL140:I:11696865:11698943:-1 gene:T22H2.5 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11697018-11698874(-) 100.0 100.0
Public Domain and Genome References
Ensembl T22H2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04754 Hypothetical protein T22H2.5b [Caenorhabditis elegans] ref|NP_492976.2| T22H2.5b [Caenorhabditis elegans] 1.0E-148
blast CAB04754 Hypothetical protein T22H2.5b [Caenorhabditis elegans] ref|NP_492976.2| T22H2.5b [Caenorhabditis elegans] 1.0E-145
blast CAB04755 Hypothetical protein T22H2.5a [Caenorhabditis elegans] ref|NP_492977.2| T22H2.5a [Caenorhabditis elegans] 1.0E-144
blast CAB04754 Hypothetical protein T22H2.5b [Caenorhabditis elegans] ref|NP_492976.2| T22H2.5b [Caenorhabditis elegans] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR005552 EMBL-EBI Scramblase 1.0E-126
Pfam IPR005552 EMBL-EBI Scramblase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000038048 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:172318_X_AT
tacgcgttcgaggagtccggatgctgtgagagacagtgctgtggaccgcaacgtggattc
gtcatgcatattgtggataactttaagagggcctaccaagccaaaattgatccacatgct
ctgaaatttcaaattttccaggaagttctgaccatcaagcgtgagttcaagtgttgcgga
ggtggttgctgtggatgcttggcatgcatcggatgctgccagcaggaatgtatcatcgag
accccatcgatgggtgttctgggtatcatccgtcagcggtgtggatgcatgtcgtcgaat
tatgatattatggatggagatggaaatgtgatcttccagattgatggtccatgctgttgc
atgctttgcggatgtcaggataaggagtttccgatcaaaaccgccaacaacggcacagtt
gtcggcgcgatcaccaaaaaatggggaggttgcttccgagaggcgttcaccgacgccgac
acgttcgccgtcaacttccccggcgacctggacgtcaaactcaaaggagtactgatagga
gccacgtttttgattgatttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACGCGTTCGAGGAGTCCGGATGCT 635 647 328 Antisense
GGACCGCAACGTGGATTCGTCATGC 14 523 370 Antisense
GCCAAAATTGATCCACATGCTCTGA 14 279 427 Antisense
GGAAGTTCTGACCATCAAGCGTGAG 91 531 468 Antisense
GGTGGTTGCTGTGGATGCTTGGCAT 204 499 508 Antisense
GGCATGCATCGGATGCTGCCAGCAG 53 537 528 Antisense
GGAATGTATCATCGAGACCCCATCG 614 529 552 Antisense
TCTGGGTATCATCCGTCAGCGGTGT 457 607 585 Antisense
TCAGCGGTGTGGATGCATGTCGTCG 510 623 600 Antisense
GCTGTTGCATGCTTTGCGGATGTCA 212 295 680 Antisense
GGAGCCACGTTTTTGATTGATTTCA 137 523 865 Antisense
Sequence Source Affymetrix Proprietary Database