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[172315_x_at]

Description E02H9.4 /REP_DB=WormBase Gene ID /WP=CE09121 /TR=Q9TZ98 /GB=AAC68770.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 12      4grp_total > 35      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 45.9
(2)
A 		9.3
(-3)
A 		48.1
(2.3)
A 		53.2
(3)
A 		44
B 13.8
(-2.3)
A 		54.3
(3)
A 		17.3
(-1.9)
A 		8.6
(-3)
A 		46
C 43.2
(-0.1)
A 		74.9
(3)
A 		13.9
(-3)
A 		53.8
(0.9)
A 		61
avr 34.3
(-0.6) 		46.2
(3) 		26.4
(-3) 		38.5
(0.7) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 18
(-1.6)
A 		13.5
(-2.3)
A 		16
(-1.9)
A 		44.5
(2.3)
A 		49.5
(3)
A 		8.4
(-3)
A 		41
B 77.3
(3)
A 		62.2
(1.7)
A 		18
(-2.2)
A 		54.3
(1)
A 		9
(-3)
A 		20.5
(-2)
A 		68
C 10.4
(-2)
A 		53.9
(3)
A 		48.9
(2.4)
A 		8.8
(-2.2)
A 		1.9
(-3)
A 		41.3
(1.5)
A 		52
avr 35.2
(0.9) 		43.2
(3) 		27.6
(-1.1) 		35.9
(1.1) 		20.1
(-3) 		23.4
(-2.1) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 16.7
(3)
A 		2
(-3)
A 		12.6
(1.3)
A 		7.8
(-0.6)
A 		15
B 4.4
(-1.7)
A 		13.4
(3)
A 		4
(-1.9)
A 		1.8
(-3)
A 		12
C 14.3
(0.4)
A 		21.5
(3)
A 		4.9
(-3)
A 		9.1
(-1.5)
A 		17
avr 11.8
(2.5) 		12.3
(3) 		7.2
(-2) 		6.2
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 3.3
(-2.4)
A 		4.8
(-1.7)
A 		3.5
(-2.3)
A 		11.3
(1.3)
A 		14.9
(3)
A 		2
(-3)
A 		13
B 26.9
(3)
A 		20.7
(1.4)
A 		4.4
(-2.8)
A 		14.4
(-0.2)
A 		3.6
(-3)
A 		7.6
(-2)
A 		23
C 3.9
(-1.8)
A 		16.5
(3)
A 		12.4
(1.5)
A 		1.8
(-2.5)
A 		0.6
(-3)
A 		13.8
(2)
A 		16
avr 11.4
(0.9) 		14
(3) 		6.8
(-2.7) 		9.2
(-0.8) 		6.4
(-3) 		7.8
(-1.9) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172315_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7520
Sequence Type Exemplar sequence
Representative Public ID E02H9.4 NCBI
Target Description E02H9.4 /REP_DB=WormBase Gene ID /WP=CE09121 /TR=Q9TZ98 /GB=AAC68770.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065159(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065159 NCBI Caenorhabditis elegans E02H9.4 (E02H9.4) mRNA, complete cds. 11/11 None
GENEFINDER00000038960 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2456759:2466788:1 9/11 None
E02H9.4 ENSEMBL cdna:known chromosome:CEL140:III:2456759:2457752:1 gene:E02H9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2456758-2457752(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E02H9.4
Entrez Gene 175365 Entrez gene
SwissProt Q9TZ98 EMBL-EBI
WormBase CE09121 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU05582 Hypothetical protein E02H9.3b [Caenorhabditis elegans] ref|NP_001021235.1| E02H9.3b [Caenorhabditis elegans] 0.0
blast AAU05581 Hypothetical protein E02H9.3a [Caenorhabditis elegans] ref|NP_001021234.1| E02H9.3a [Caenorhabditis elegans] 0.0
blast T33601 hypothetical protein E02H9.3 - Caenorhabditis elegans 0.0
blast AAC68770 Hypothetical protein E02H9.4 [Caenorhabditis elegans] ref|NP_497560.1| E02H9.4 [Caenorhabditis elegans] 1.0E-66
blast AAK29788 Hypothetical protein C49A9.1 [Caenorhabditis elegans] ref|NP_500938.1| C49A9.1 [Caenorhabditis elegans] 7.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 3.2E-14
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 1.2E-77
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 2.1E-56
Sequence
Target Sequence 
>C. ELEGANS:172315_X_AT
atgctcatctaccataactgcaccacggaccggactatgagaaagatgagacatgaatgg
ggaacatttggcattcgtattgcagatgctttttcaacgatgaacattgatgtttttgac
aaaggagagaaatacaacggtaatgaaaaagtggaacttacaactactgtcaaaaattat
aaaccaaagaataacacaagaaaatggccagcagttgaagcaaagtatttaggagttgta
tatgacaaccacaataaacttcccaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTCATCTACCATAACTGCACCA 673 39 13 Antisense
TCATCTACCATAACTGCACCACGGA 85 621 17 Antisense
ATAACTGCACCACGGACCGGACTAT 346 23 26 Antisense
ACCACGGACCGGACTATGAGAAAGA 605 83 34 Antisense
GGGGAACATTTGGCATTCGTATTGC 27 499 71 Antisense
ATTCGTATTGCAGATGCTTTTTCAA 587 9 85 Antisense
GATGCTTTTTCAACGATGAACATTG 58 413 97 Antisense
GAACATTGATGTTTTTGACAAAGGA 352 351 114 Antisense
AGTGGAACTTACAACTACTGTCAAA 645 55 162 Antisense
GAAAATGGCCAGCAGTTGAAGCAAA 11 355 212 Antisense
GACAACCACAATAAACTTCCCAAGA 58 365 256 Antisense
Sequence Source Affymetrix Proprietary Database