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[172313_x_at]

Description B0546.5 /REP_DB=WormBase Gene ID /WP=CE16796 /TR=O44442 /GB=AAB92014.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1060
1 2 3 4 range
A 85.7
(-2.6)
A 		31.9
(-3)
A 		65.7
(-2.8)
A 		861.2
(3)
P 		829
B 128.3
(-2.3)
A 		38.7
(-3)
A 		77.1
(-2.7)
A 		786.9
(3)
P 		748
C 91.4
(-2.6)
A 		59.7
(-2.8)
A 		27
(-3)
A 		1086.9
(3)
P 		1060
avr 101.8
(-2.6) 		43.4
(-3) 		56.6
(-2.9) 		911.7
(3) 		868

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 250
1 2 3 4 5 6 range
A 74.2
(-1.1)
A 		6.6
(-3)
A 		80.3
(-0.9)
A 		62.7
(-1.4)
A 		195
(2.3)
P 		218.2
(3)
P 		212
B 70
(-2.4)
A 		94.9
(-1.7)
A 		48.9
(-3)
A 		113.7
(-1.1)
P 		165.4
(0.4)
P 		256.4
(3)
P 		208
C 38.3
(-2.8)
A 		109.2
(1.7)
A 		35.9
(-3)
A 		121.2
(2.4)
M 		114.1
(2)
P 		129.9
(3)
P 		94
avr 60.8
(-2.8) 		70.2
(-2.4) 		55
(-3) 		99.2
(-1.2) 		158.2
(1.2) 		201.5
(3) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 31.2
(-1.8)
A 		6.9
(-3)
A 		17.2
(-2.5)
A 		126.1
(3)
P 		119
B 40.9
(-1.8)
A 		9.6
(-3)
A 		17.9
(-2.7)
A 		167.7
(3)
P 		158
C 30.2
(-2.3)
A 		17.2
(-2.7)
A 		9.4
(-3)
A 		183.3
(3)
P 		174
avr 34.1
(-2.1) 		11.2
(-3) 		14.8
(-2.9) 		159
(3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 13.7
(-1.8)
A 		2.4
(-3)
A 		17.5
(-1.4)
A 		15.9
(-1.6)
A 		58.8
(3)
P 		53.1
(2.4)
P 		56
B 24.4
(-2.1)
A 		31.6
(-1.6)
A 		11.9
(-3)
A 		30.1
(-1.7)
P 		67.2
(1)
P 		95.6
(3)
P 		84
C 14.2
(-2.1)
A 		33.3
(1.2)
A 		9.1
(-3)
A 		24.4
(-0.3)
M 		36.9
(1.8)
P 		43.5
(3)
P 		34
avr 17.4
(-2.5) 		22.4
(-1.9) 		12.8
(-3) 		23.5
(-1.7) 		54.3
(1.9) 		64.1
(3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172313_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10652
Sequence Type Exemplar sequence
Representative Public ID B0546.5 NCBI
Target Description B0546.5 /REP_DB=WormBase Gene ID /WP=CE16796 /TR=O44442 /GB=AAB92014.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067936(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067936 NCBI Caenorhabditis elegans B0546.5 (B0546.5) mRNA, complete cds. 9/11 A 
B0546.5 ENSEMBL cdna:known chromosome:CEL140:IV:3388073:3390084:-1 gene:B0546.5 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_059871 8/11 Cross Hyb Matching Probes A 
NM_060590 7/11 Cross Hyb Matching Probes A 
SNAP00000041138 3/11 Cross Hyb Matching Probes None
GENEFINDER00000041145 7/11 Cross Hyb Matching Probes None
GENEFINDER00000036932 8/11 Cross Hyb Matching Probes None
SNAP00000036929 6/11 Cross Hyb Matching Probes None
SNAP00000001412 3/11 Cross Hyb Matching Probes None
F16D3.6 8/11 Cross Hyb Matching Probes A 
F35E2.1 7/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8383545-8385591(+) 94.7 94.7
chrI:11746778-11748815(-) 97.9 97.9
chrIV:3388052-3390090(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0546.5
Entrez Gene 182031 Entrez gene
SwissProt O44442 EMBL-EBI
WormBase CE34640 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB92014 Hypothetical protein B0546.5 [Caenorhabditis elegans] ref|NP_500337.2| B0546.5 [Caenorhabditis elegans] 1.0E-136
blast CAB04282 Hypothetical protein F35E2.1 [Caenorhabditis elegans] ref|NP_492991.1| F35E2.1 [Caenorhabditis elegans] 1.0E-125
blast CAB01498 Hypothetical protein F16D3.6 [Caenorhabditis elegans] ref|NP_492272.2| F16D3.6 [Caenorhabditis elegans] 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.0E-126
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 2.0E-45
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500337.2 4 44-66,79-101,155-177,198-220
B0546.5 4 44-66,79-101,155-177,198-220
Sequence
Target Sequence 
>C. ELEGANS:172313_X_AT
actaaactggaaccttatccatgtaaaattagctagatccattatttacgcgtttctcaa
ttatccatttggggaaaacaataagaatgatgtgtgttatacacttcgattcagtaatgg
aatggaacttgttcggatcttctcaacgttatttttcaattttgcagccatcattgtcaa
ttttgtaattcttcgatttgtgaaacactttgaaagtgagaataaaaatatccaatcaaa
aagaattcaattaactcaaagtcttctcattcaatcagtaataccagtcattgtatcttt
gcctttattatttttatcatttgagttctattttggaatcccaatgccaagttcatttgg
atccacatggtatgctctaacattccttgggccatttctcatgccactctcgtcaattct
cggaattcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTAAACTGGAACCTTATCCATGTA 71 99 534 Antisense
AGCTAGATCCATTATTTACGCGTTT 360 79 564 Antisense
TTACGCGTTTCTCAATTATCCATTT 483 681 579 Antisense
GAACTTGTTCGGATCTTCTCAACGT 180 349 658 Antisense
ATTTTGCAGCCATCATTGTCAATTT 507 13 692 Antisense
ACTCAAAGTCTTCTCATTCAATCAG 153 99 787 Antisense
ACCAGTCATTGTATCTTTGCCTTTA 82 87 816 Antisense
GGAATCCCAATGCCAAGTTCATTTG 43 531 868 Antisense
GGATCCACATGGTATGCTCTAACAT 63 513 892 Antisense
TAACATTCCTTGGGCCATTTCTCAT 400 639 911 Antisense
CACTCTCGTCAATTCTCGGAATTCG 192 197 938 Antisense
Sequence Source Affymetrix Proprietary Database