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[172304_x_at]

Description C32E8.4 /REP_DB=WormBase Gene ID /WP=CE08528 /TR=P91126 /GB=AAB42319.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 32      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 19.7
(-0.4)
A 		34
(3)
A 		9.1
(-3)
A 		29.6
(1.9)
A 		25
B 6.4
(-3)
A 		32.6
(3)
A 		29.9
(2.4)
A 		14.9
(-1.1)
A 		26
C 10.6
(-3)
A 		16.1
(-2.4)
A 		52.2
(1.8)
A 		62.8
(3)
A 		52
avr 12.2
(-3) 		27.6
(0.9) 		30.4
(1.6) 		35.8
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 334
1 2 3 4 5 6 range
A 19
(-3)
A 		22.9
(-2.9)
A 		19.8
(-3)
A 		27.7
(-2.7)
A 		177
(1.7)
A 		219.6
(3)
M 		201
B 8.3
(-3)
A 		10.8
(-2.9)
A 		85.3
(-1.2)
A 		24.3
(-2.6)
A 		225.4
(2.1)
M 		263.5
(3)
P 		255
C 21.1
(-3)
A 		20.5
(-3)
A 		81.1
(-1.9)
A 		68.8
(-2.1)
A 		257.6
(1.4)
P 		342.1
(3)
P 		322
avr 16.1
(-3) 		18.1
(-3) 		62.1
(-1.9) 		40.3
(-2.4) 		220
(1.7) 		275.1
(3) 		259
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 7.2
(2.8)
A 		7.4
(3)
A 		2.4
(-3)
A 		4.3
(-0.7)
A 		5
B 2.1
(-3)
A 		8
(3)
A 		6.9
(1.9)
A 		3.2
(-1.9)
A 		6
C 3.5
(-3)
A 		4.6
(-2.6)
A 		18.2
(3)
A 		10.6
(-0.1)
A 		15
avr 4.3
(-3) 		6.7
(-0.1) 		9.2
(3) 		6
(-0.9) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 3.5
(-3)
A 		8.1
(-2.4)
A 		4.3
(-2.9)
A 		7
(-2.6)
A 		53.4
(3)
A 		53.5
(3)
M 		50
B 2.9
(-3)
A 		3.6
(-3)
A 		20.7
(-1.9)
A 		6.4
(-2.8)
A 		91.6
(2.6)
M 		98.3
(3)
P 		95
C 7.8
(-2.9)
A 		6.3
(-3)
A 		20.6
(-2.2)
A 		13.8
(-2.6)
A 		83.3
(1.3)
P 		114.5
(3)
P 		108
avr 4.7
(-3) 		6
(-2.9) 		15.2
(-2.3) 		9.1
(-2.7) 		76.1
(2.1) 		88.8
(3) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172304_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.143
Sequence Type Exemplar sequence
Representative Public ID C32E8.4 NCBI
Target Description C32E8.4 /REP_DB=WormBase Gene ID /WP=CE08528 /TR=P91126 /GB=AAB42319.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058817(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058817 NCBI Caenorhabditis elegans C32E8.4 (C32E8.4) mRNA, complete cds. 10/11 None
SNAP00000040650 ENSEMBL cdna:SNAP chromosome:CEL140:I:3783944:3784177:1 9/11 None
GENEFINDER00000040663 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3783944:3784438:1 11/11 None
C32E8.4 ENSEMBL cdna:known chromosome:CEL140:I:3783837:3784437:1 gene:C32E8.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069622 5/11 Cross Hyb Matching Probes A 
GENEFINDER00000018147 1/11 Cross Hyb Matching Probes None
SNAP00000017045 5/11 Cross Hyb Matching Probes None
GENEFINDER00000017057 5/11 Cross Hyb Matching Probes None
F13E9.8 5/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3783943-3784438(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32E8.4
Entrez Gene 171947 Entrez gene
SwissProt P91126 EMBL-EBI
WormBase CE29692 Wormbase
Sequence
Target Sequence 
>C. ELEGANS:172304_X_AT
ctcttcttctctggaagcactctgctcgttcaatgtggtggaaagaaaaagggagcaact
tctgccgaaggaaaatcttcgacgatgggcccggctcctggaggagctcctgctgctgct
tccgctcaaggagaacctgccgtcatctccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTTCTTCTCTGGAAGCACTCTGC 670 229 43 Antisense
CTTCTTCTCTGGAAGCACTCTGCTC 418 221 45 Antisense
TCTGGAAGCACTCTGCTCGTTCAAT 373 607 52 Antisense
AGCACTCTGCTCGTTCAATGTGGTG 398 77 58 Antisense
CTCTGCTCGTTCAATGTGGTGGAAA 627 231 62 Antisense
AAAAGGGAGCAACTTCTGCCGAAGG 232 131 89 Antisense
GCAACTTCTGCCGAAGGAAAATCTT 457 319 97 Antisense
GGAAAATCTTCGACGATGGGCCCGG 100 529 112 Antisense
TGCTGCTTCCGCTCAAGGAGAACCT 67 581 156 Antisense
TGCTTCCGCTCAAGGAGAACCTGCC 623 579 159 Antisense
CAAGGAGAACCTGCCGTCATCTCCG 30 185 169 Antisense
Sequence Source Affymetrix Proprietary Database