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[172275_x_at]

Description W07A8.3 /REP_DB=WormBase Gene ID /WP=CE27221 /TR=O62397 /GB=CAB04929.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 59      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 81      6grp_total > 38      4grp_total > 61      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 328.3
(3)
P 		268.6
(-3)
P 		293.5
(-0.5)
P 		273.3
(-2.5)
P 		60
B 272.3
(0)
P 		326.9
(3)
P 		287.6
(0.8)
P 		217.7
(-3)
P 		109
C 316.1
(3)
P 		247.6
(-0.1)
P 		287.3
(1.7)
P 		184.7
(-3)
P 		131
avr 305.6
(3) 		281
(1.2) 		289.5
(1.8) 		225.2
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 334.6
(0.8)
P 		333
(0.7)
P 		254.7
(-3)
P 		300.4
(-0.8)
P 		377.4
(2.8)
P 		381
(3)
P 		126
B 305.6
(0.1)
P 		387.8
(3)
P 		245.4
(-2)
P 		277.6
(-0.9)
P 		217.6
(-3)
P 		342.8
(1.4)
P 		170
C 358.8
(3)
P 		296.4
(-0.3)
P 		272.6
(-1.6)
P 		322.1
(1.1)
P 		307.3
(0.3)
P 		245.5
(-3)
P 		113
avr 333
(2.6) 		339.1
(3) 		257.6
(-3) 		300
(0.1) 		300.8
(0.2) 		323.1
(1.8) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 119.6
(3)
P 		58.3
(-1.6)
P 		76.7
(-0.2)
P 		40
(-3)
P 		80
B 86.8
(3)
P 		80.7
(2.1)
P 		66.6
(-0)
P 		46.4
(-3)
P 		40
C 104.6
(3)
P 		71.2
(0.3)
P 		100.1
(2.6)
P 		31.1
(-3)
P 		74
avr 103.7
(3) 		70.1
(-0.1) 		81.1
(0.9) 		39.2
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 61.7
(-2.4)
P 		118.4
(3)
P 		55.5
(-3)
P 		76.2
(-1)
P 		113.9
(2.6)
P 		92.8
(0.6)
P 		63
B 106.3
(1)
P 		129.1
(3)
P 		59.6
(-3)
P 		73.5
(-1.8)
P 		88.4
(-0.5)
P 		127.8
(2.9)
P 		70
C 133
(3)
P 		90.5
(-0.7)
P 		69.4
(-2.6)
P 		64.8
(-3)
P 		99.4
(0)
P 		82.2
(-1.5)
P 		68
avr 100.3
(1.5) 		112.7
(3) 		61.5
(-3) 		71.5
(-1.8) 		100.6
(1.6) 		100.9
(1.6) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172275_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13401
Sequence Type Exemplar sequence
Representative Public ID W07A8.3 NCBI
Target Description W07A8.3 /REP_DB=WormBase Gene ID /WP=CE27221 /TR=O62397 /GB=CAB04929.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075601(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075601 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-25) (dnj-25) mRNA, complete cds. 11/11 A 
GENEFINDER00000017810 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20728069:20742380:-1 11/11 None
W07A8.3.1 ENSEMBL cdna:known chromosome:CEL140:V:20728000:20742390:-1 gene:W07A8.3 11/11 A 
W07A8.3.2 ENSEMBL cdna:known chromosome:CEL140:V:20728010:20742391:-1 gene:W07A8.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017803 7/11 Cross Hyb Matching Probes None
SNAP00000017807 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20728319-20742875(-) 93.26 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-25
Ensembl W07A8.3
Entrez Gene 180356 Entrez gene
SwissProt O62397 EMBL-EBI
WormBase CE27221 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04929 Hypothetical protein W07A8.3 [Caenorhabditis elegans] ref|NP_508002.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-25) [Caenorhabditis elegans] gb|AAG02478.1| auxilin [Caenorhabditis elegans] 0.0
blast CAE61644 Hypothetical protein CBG05578 [Caenorhabditis briggsae] 0.0
blast XP_424873 PREDICTED: similar to Cyclin G-associated kinase [Gallus gallus] 6.0E-35
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 0.0
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.2E-9
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.2E-9
Sequence
Target Sequence 
>C. ELEGANS:172275_X_AT
gacaagcggcacctcaaaatacgcagaatgtatcgcaaaagacgcaacaaacaagaccac
agaatacgtacaaaccagcatttggtgacgcggcgacgggatctgcgaaggtttcaatgg
atgcattcagtgatttgttgaatcaggggggcttcaaaccaacacaaagggaaaatcaaa
agcagagtattgggaatatgctgaaagttgaacaatcgaaaaatctgacaccggaagaga
ttcaggtaaaaaatcgaaaaattttaattagttctgcaaaacttgaatttctacctaaaa
gtttgcagattcgagactggactcaaggcaaagagcgtaatattcgtgctcttctcggct
cacttcataatgtcctttgggagggagctgatcgatggaatcagccatcgatgggtgatc
ttttgacacctgatcaggtaaaatttgtgatattggccttttttttttatttattaaaaa
tattaatcaagaaacattaccgcaaggcatgtcttgtggtacatccagacaaattgacag
gatctcctcatctctcattggccaaaatggcgttcacggagcttaacgatgcctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAGCGGCACCTCAAAATACGCA 331 365 2261 Antisense
AACCAGCATTTGGTGACGCGGCGAC 128 149 2333 Antisense
ACGCGGCGACGGGATCTGCGAAGGT 687 89 2348 Antisense
ATCAGGGGGGCTTCAAACCAACACA 512 23 2402 Antisense
GAGCGTAATATTCGTGCTCTTCTCG 526 385 2593 Antisense
TTCTCGGCTCACTTCATAATGTCCT 630 691 2612 Antisense
CAGCCATCGATGGGTGATCTTTTGA 295 203 2662 Antisense
AGGCATGTCTTGTGGTACATCCAGA 60 53 2765 Antisense
AATTGACAGGATCTCCTCATCTCTC 700 177 2792 Antisense
TCCTCATCTCTCATTGGCCAAAATG 292 593 2805 Antisense
GTTCACGGAGCTTAACGATGCCTAT 139 445 2832 Antisense
Sequence Source Affymetrix Proprietary Database