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[172255_x_at]

Description Y20C6A.3 /REP_DB=WormBase Gene ID /WP=CE16601 /TR=O45914 /GB=CAA16304.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 11      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 44.2
(-2.2)
A 		42.7
(-2.3)
A 		26.5
(-3)
A 		157.9
(3)
P 		131
B 76.3
(-0.6)
P 		55.6
(-3)
A 		85.5
(0.4)
A 		108.1
(3)
P 		53
C 44.9
(-2)
A 		71.9
(-1.1)
A 		17.3
(-3)
A 		185.5
(3)
P 		168
avr 55.1
(-2.3) 		56.7
(-2.2) 		43.1
(-3) 		150.5
(3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 52.2
(3)
A 		11.1
(-2.4)
A 		33.2
(0.5)
A 		6.5
(-3)
A 		37.4
(1.1)
A 		31.1
(0.2)
A 		46
B 23.1
(0.3)
A 		35.1
(2.4)
A 		36.2
(2.6)
A 		4.6
(-3)
A 		38.5
(3)
A 		19.7
(-0.3)
A 		34
C 25.8
(-1.5)
A 		57.9
(0.7)
A 		5.2
(-3)
A 		90.1
(3)
A 		18.3
(-2.1)
A 		15.5
(-2.3)
A 		85
avr 33.7
(2.5) 		34.7
(3) 		24.9
(-1.7) 		33.7
(2.5) 		31.4
(1.4) 		22.1
(-3) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 16.1
(0.4)
A 		9.3
(-2.1)
A 		6.9
(-3)
A 		23.1
(3)
P 		16
B 24.3
(3)
P 		13.7
(-3)
A 		19.8
(0.5)
A 		23
(2.3)
P 		11
C 14.9
(-0.9)
A 		20.7
(0.5)
A 		6
(-3)
A 		31.3
(3)
P 		25
avr 18.4
(0) 		14.6
(-1.5) 		10.9
(-3) 		25.8
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 9.6
(1.9)
A 		3.9
(-1.6)
A 		7.2
(0.4)
A 		1.7
(-3)
A 		11.3
(3)
A 		7.6
(0.7)
A 		10
B 8
(-0.2)
A 		11.7
(1.3)
A 		8.8
(0.1)
A 		1.2
(-3)
A 		15.7
(3)
A 		7.3
(-0.5)
A 		15
C 9.5
(-0.1)
A 		17.7
(2.9)
A 		1.3
(-3)
A 		18.1
(3)
A 		5.9
(-1.4)
A 		5.2
(-1.6)
A 		17
avr 9
(0.6) 		11.1
(3) 		5.8
(-3) 		7
(-1.6) 		11
(2.9) 		6.7
(-2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172255_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13492
Sequence Type Exemplar sequence
Representative Public ID Y20C6A.3 NCBI
Target Description Y20C6A.3 /REP_DB=WormBase Gene ID /WP=CE16601 /TR=O45914 /GB=CAA16304.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074998(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074998 NCBI Caenorhabditis elegans Y20C6A.3 (Y20C6A.3) mRNA, complete cds. 11/11 None
SNAP00000029066 ENSEMBL cdna:SNAP chromosome:CEL140:V:17384814:17385941:1 11/11 None
GENEFINDER00000029070 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17385189:17385950:1 11/11 None
Y20C6A.3 ENSEMBL cdna:known chromosome:CEL140:V:17384667:17385950:1 gene:Y20C6A.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075180 2/11 Cross Hyb Matching Probes A 
NM_067889 3/11 Cross Hyb Matching Probes A 
NM_075916 6/11 Cross Hyb Matching Probes A 
NM_076519 1/11 Cross Hyb Matching Probes A 
SNAP00000001616 3/11 Cross Hyb Matching Probes None
GENEFINDER00000001632 3/11 Cross Hyb Matching Probes None
SNAP00000039472 2/11 Cross Hyb Matching Probes None
GENEFINDER00000039486 2/11 Cross Hyb Matching Probes None
SNAP00000029103 2/11 Cross Hyb Matching Probes None
GENEFINDER00000029109 3/11 Cross Hyb Matching Probes None
SNAP00000009970 6/11 Cross Hyb Matching Probes None
GENEFINDER00000009990 6/11 Cross Hyb Matching Probes None
Y67D8C.2 3/11 Cross Hyb Matching Probes A 
Y51A2A.5 2/11 Cross Hyb Matching Probes A 
F07G6.3 6/11 Cross Hyb Matching Probes A 
C03F11.4.1 1/11 Cross Hyb Matching Probes A 
C03F11.4.2 1/11 Cross Hyb Matching Probes A 
C03F11.4.3 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17384666-17385950(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y20C6A.3
Entrez Gene 189499 Entrez gene
SwissProt O45914 EMBL-EBI
WormBase CE16601 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA16304 Hypothetical protein Y20C6A.3 [Caenorhabditis elegans] ref|NP_507399.1| Y20C6A.3 [Caenorhabditis elegans] 0.0
blast AAG23381 Hypothetical protein Y67D8C.2 [Caenorhabditis elegans] ref|NP_500290.1| Y67D8C.2 [Caenorhabditis elegans] 1.0E-173
blast CAA16304 Hypothetical protein Y20C6A.3 [Caenorhabditis elegans] ref|NP_507399.1| Y20C6A.3 [Caenorhabditis elegans] 1.0E-161
blast CAA16304 Hypothetical protein Y20C6A.3 [Caenorhabditis elegans] ref|NP_507399.1| Y20C6A.3 [Caenorhabditis elegans] 1.0E-153
blast AAG23381 Hypothetical protein Y67D8C.2 [Caenorhabditis elegans] ref|NP_500290.1| Y67D8C.2 [Caenorhabditis elegans] 1.0E-132
blast AAG23381 Hypothetical protein Y67D8C.2 [Caenorhabditis elegans] ref|NP_500290.1| Y67D8C.2 [Caenorhabditis elegans] 1.0E-131
blast NP_508317 F07G6.3 [Caenorhabditis elegans] gb|AAA96074.1| Hypothetical protein F07G6.3 [Caenorhabditis elegans] 1.0E-118
Sequence
Target Sequence 
>C. ELEGANS:172255_X_AT
cactgatgccattcacaaggaaagcacacaaattatgtcatttacggaaaaatcctgtga
caatgaaaatgataagattgagcacattatgcacaactcattgaacaatcagatacaaaa
ggccggactacaaaagccgggagatgtcgcctgtggcggattttttgacaagaaaatgaa
gcgtagagtcaatgatgaaatgcgtccacgtacagatccaccaactgacttgtcacaaca
agcatgctatggtacggtatctgatatgttggattggaataattggagtcatgagaattg
gaaggatatgagttggcaaagattcgttcgagaatcacgtcctcgcaaggatccaccttg
gagttcgtgtcgttcggaagttggcatgtgttatggatggagatgggatgttagtcaagt
tccatcatgtcttcaagactacggaagtcgtcacgtct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTGATGCCATTCACAAGGAAAGC 293 195 426 Antisense
CAAAAGCCGGGAGATGTCGCCTGTG 168 189 556 Antisense
TCGCCTGTGGCGGATTTTTTGACAA 427 599 572 Antisense
GAAATGCGTCCACGTACAGATCCAC 423 361 622 Antisense
ACAGATCCACCAACTGACTTGTCAC 397 107 637 Antisense
GCTATGGTACGGTATCTGATATGTT 392 303 671 Antisense
GAGAATCACGTCCTCGCAAGGATCC 388 391 755 Antisense
ATCCACCTTGGAGTTCGTGTCGTTC 169 41 776 Antisense
CGTTCGGAAGTTGGCATGTGTTATG 117 247 796 Antisense
GTCAAGTTCCATCATGTCTTCAAGA 58 463 839 Antisense
CAAGACTACGGAAGTCGTCACGTCT 216 183 859 Antisense
Sequence Source Affymetrix Proprietary Database